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Silencing of mitochondrial NADP+-dependent isocitrate dehydrogenase by small interfering RNA enhances heat shock-induced apoptosis.
Biochem Biophys Res Commun. 2008 Feb 22; 366(4):1012-8.BB

Abstract

Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. Mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) produces NADPH, an essential reducing equivalent for the antioxidant system. In this report, we demonstrate that silencing of IDPm expression in HeLa cells greatly enhances apoptosis induced by heat shock. Transfection of HeLa cells with an IDPm small interfering RNA (siRNA) markedly decreased activity of IDPm, enhancing the susceptibility of heat shock-induced apoptosis reflected by morphological evidence of apoptosis, DNA fragmentation, cellular redox status, mitochondria redox status and function, and the modulation of apoptotic marker proteins. These results indicate that IDPm may play an important role in regulating the apoptosis induced by heat shock and the sensitizing effect of IDPm siRNA on the apoptotic cell death of HeLa cells offers the possibility of developing a modifier of cancer therapy.

Authors+Show Affiliations

School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Republic of Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18096511

Citation

Shin, Seoung Woo, et al. "Silencing of Mitochondrial NADP+-dependent Isocitrate Dehydrogenase By Small Interfering RNA Enhances Heat Shock-induced Apoptosis." Biochemical and Biophysical Research Communications, vol. 366, no. 4, 2008, pp. 1012-8.
Shin SW, Kil IS, Park JW. Silencing of mitochondrial NADP+-dependent isocitrate dehydrogenase by small interfering RNA enhances heat shock-induced apoptosis. Biochem Biophys Res Commun. 2008;366(4):1012-8.
Shin, S. W., Kil, I. S., & Park, J. W. (2008). Silencing of mitochondrial NADP+-dependent isocitrate dehydrogenase by small interfering RNA enhances heat shock-induced apoptosis. Biochemical and Biophysical Research Communications, 366(4), 1012-8.
Shin SW, Kil IS, Park JW. Silencing of Mitochondrial NADP+-dependent Isocitrate Dehydrogenase By Small Interfering RNA Enhances Heat Shock-induced Apoptosis. Biochem Biophys Res Commun. 2008 Feb 22;366(4):1012-8. PubMed PMID: 18096511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silencing of mitochondrial NADP+-dependent isocitrate dehydrogenase by small interfering RNA enhances heat shock-induced apoptosis. AU - Shin,Seoung Woo, AU - Kil,In Sup, AU - Park,Jeen-Woo, Y1 - 2007/12/27/ PY - 2007/12/01/received PY - 2007/12/09/accepted PY - 2007/12/22/pubmed PY - 2008/2/6/medline PY - 2007/12/22/entrez SP - 1012 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 366 IS - 4 N2 - Heat shock may increase oxidative stress due to increased production of reactive oxygen species and/or the promotion of cellular oxidation events. Mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) produces NADPH, an essential reducing equivalent for the antioxidant system. In this report, we demonstrate that silencing of IDPm expression in HeLa cells greatly enhances apoptosis induced by heat shock. Transfection of HeLa cells with an IDPm small interfering RNA (siRNA) markedly decreased activity of IDPm, enhancing the susceptibility of heat shock-induced apoptosis reflected by morphological evidence of apoptosis, DNA fragmentation, cellular redox status, mitochondria redox status and function, and the modulation of apoptotic marker proteins. These results indicate that IDPm may play an important role in regulating the apoptosis induced by heat shock and the sensitizing effect of IDPm siRNA on the apoptotic cell death of HeLa cells offers the possibility of developing a modifier of cancer therapy. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/18096511/Silencing_of_mitochondrial_NADP+_dependent_isocitrate_dehydrogenase_by_small_interfering_RNA_enhances_heat_shock_induced_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(07)02693-9 DB - PRIME DP - Unbound Medicine ER -