Tags

Type your tag names separated by a space and hit enter

NKG2C is a major triggering receptor involved in the V[delta]1 T cell-mediated cytotoxicity against HIV-infected CD4 T cells.
AIDS. 2008 Jan 11; 22(2):217-26.AIDS

Abstract

BACKGROUND

Gammadelta T cells share with natural killer (NK) cells many effector capabilities and cell-surface proteins, including the NKG2 receptor family. A subset of gammadelta T cells that express the variable Vdelta1 region plays a critical role in immune regulation, tumour surveillance and viral infection. Dramatic expansion of Vdelta1 T cells has been observed in HIV disease.

OBJECTIVE

To determine if NKG2C expression on Vdelta1 T cells during HIV-1 infection is correlated with CD4 cell count and involved in lysis of CD4 T cells.

METHODS

gammadelta T cells from viraemic HIV-infected patients were examined. Expression of NK cell markers was analyzed by flow cytometry. The cytolytic activity of Vdelta1 T cells was determined by either Cr-release assays or degranulation assays against HLA-E-transfected 721.221 cells or HIV-infected CD4 primary T cells.

RESULTS

The expression of C-type lectin NKG2 receptors was sharply modulated on gammadelta T cells in patients with HIV infection. A profound decrease of Vdelta1 T cells bearing inhibitory NKG2A receptors corresponded to a drastic expansion of a distinct population of Vdelta1 T cells expressing a functional activating NKG2C receptor. Engagement of HLA-E, the ligand of both NKG2A and NKG2C, which is specifically induced on HIV-infected CD4 T cells, substantially enhanced the Vdelta1 T cell-mediated cytotoxicity.

CONCLUSIONS

These results raise the possibility that induction of NKG2C expression on Vdelta1 T cells plays a key role in the destruction of HIV-infected CD4 T cells during HIV disease.

Authors+Show Affiliations

Institut National de la Santé et de la Recherche Scientifique U543, Université Pierre et Marie Curie Paris-6, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18097224

Citation

Fausther-Bovendo, Hugues, et al. "NKG2C Is a Major Triggering Receptor Involved in the V[delta]1 T Cell-mediated Cytotoxicity Against HIV-infected CD4 T Cells." AIDS (London, England), vol. 22, no. 2, 2008, pp. 217-26.
Fausther-Bovendo H, Wauquier N, Cherfils-Vicini J, et al. NKG2C is a major triggering receptor involved in the V[delta]1 T cell-mediated cytotoxicity against HIV-infected CD4 T cells. AIDS. 2008;22(2):217-26.
Fausther-Bovendo, H., Wauquier, N., Cherfils-Vicini, J., Cremer, I., Debré, P., & Vieillard, V. (2008). NKG2C is a major triggering receptor involved in the V[delta]1 T cell-mediated cytotoxicity against HIV-infected CD4 T cells. AIDS (London, England), 22(2), 217-26.
Fausther-Bovendo H, et al. NKG2C Is a Major Triggering Receptor Involved in the V[delta]1 T Cell-mediated Cytotoxicity Against HIV-infected CD4 T Cells. AIDS. 2008 Jan 11;22(2):217-26. PubMed PMID: 18097224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NKG2C is a major triggering receptor involved in the V[delta]1 T cell-mediated cytotoxicity against HIV-infected CD4 T cells. AU - Fausther-Bovendo,Hugues, AU - Wauquier,Nadia, AU - Cherfils-Vicini,Julien, AU - Cremer,Isabelle, AU - Debré,Patrice, AU - Vieillard,Vincent, PY - 2007/12/22/pubmed PY - 2008/2/1/medline PY - 2007/12/22/entrez SP - 217 EP - 26 JF - AIDS (London, England) JO - AIDS VL - 22 IS - 2 N2 - BACKGROUND: Gammadelta T cells share with natural killer (NK) cells many effector capabilities and cell-surface proteins, including the NKG2 receptor family. A subset of gammadelta T cells that express the variable Vdelta1 region plays a critical role in immune regulation, tumour surveillance and viral infection. Dramatic expansion of Vdelta1 T cells has been observed in HIV disease. OBJECTIVE: To determine if NKG2C expression on Vdelta1 T cells during HIV-1 infection is correlated with CD4 cell count and involved in lysis of CD4 T cells. METHODS: gammadelta T cells from viraemic HIV-infected patients were examined. Expression of NK cell markers was analyzed by flow cytometry. The cytolytic activity of Vdelta1 T cells was determined by either Cr-release assays or degranulation assays against HLA-E-transfected 721.221 cells or HIV-infected CD4 primary T cells. RESULTS: The expression of C-type lectin NKG2 receptors was sharply modulated on gammadelta T cells in patients with HIV infection. A profound decrease of Vdelta1 T cells bearing inhibitory NKG2A receptors corresponded to a drastic expansion of a distinct population of Vdelta1 T cells expressing a functional activating NKG2C receptor. Engagement of HLA-E, the ligand of both NKG2A and NKG2C, which is specifically induced on HIV-infected CD4 T cells, substantially enhanced the Vdelta1 T cell-mediated cytotoxicity. CONCLUSIONS: These results raise the possibility that induction of NKG2C expression on Vdelta1 T cells plays a key role in the destruction of HIV-infected CD4 T cells during HIV disease. SN - 1473-5571 UR - https://www.unboundmedicine.com/medline/citation/18097224/NKG2C_is_a_major_triggering_receptor_involved_in_the_V[delta]1_T_cell_mediated_cytotoxicity_against_HIV_infected_CD4_T_cells_ L2 - https://doi.org/10.1097/QAD.0b013e3282f46e7c DB - PRIME DP - Unbound Medicine ER -