Tags

Type your tag names separated by a space and hit enter

Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene.
Development. 1991 Dec; 113(4):1257-65.D

Abstract

By immunofluorescence on cytospin preparations and on semithin sections of mouse pancreatic buds, we have found glucagon and pancreatic polypeptide (PP)-containing cells at embryonal day 10.5 (E 10.5) in dorsal buds and at E 11.5 in ventral buds. Insulin-containing cells appear in dorsal buds at E 11.5, and one to two days later in ventral buds. Somatostatin-containing cells are detectable from E 13.5 in both dorsal and ventral buds. A quantitative analysis shows that up to E 15.5, PP-containing cells are relatively abundant in both buds. By PCR amplification of oligo(dT)-primed cDNAs prepared from total pancreatic RNA, we also detect PP mRNA from E 10.5 onwards, thus confirming the early expression of the PP gene in the developing mouse pancreas. Analysis of endocrine cells in situ suggests three major patterns of cell distribution in embryonic pancreas. First, individual hormone-containing cells are located within the epithelium of pancreatic ducts. In both dorsal and ventral buds, the majority of these endocrine cells contain PP, but many also contain glucagon, insulin or somatostatin. Secondly, clusters of endocrine cells are found in the pancreatic interstitium. Many of these cells contain both glucagon and PP which, by immunogold labelling of consecutive thin sections, can be shown to co-exist within individual secretory granules. Finally, starting on E 18.5, typical islets are formed with centrally located B cells and with the adult 'one cell-one hormone' phenotype. These results suggest an intriguing ontogenic relationship between A- and PP-cells, and also indicate that PP-containing cells may occupy a hitherto unexpected place in the lineage of endocrine islet cells.

Authors+Show Affiliations

Institute of Histology and Embryology, University of Geneva Medical School, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1811941

Citation

Herrera, P L., et al. "Embryogenesis of the Murine Endocrine Pancreas; Early Expression of Pancreatic Polypeptide Gene." Development (Cambridge, England), vol. 113, no. 4, 1991, pp. 1257-65.
Herrera PL, Huarte J, Sanvito F, et al. Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. Development. 1991;113(4):1257-65.
Herrera, P. L., Huarte, J., Sanvito, F., Meda, P., Orci, L., & Vassalli, J. D. (1991). Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. Development (Cambridge, England), 113(4), 1257-65.
Herrera PL, et al. Embryogenesis of the Murine Endocrine Pancreas; Early Expression of Pancreatic Polypeptide Gene. Development. 1991;113(4):1257-65. PubMed PMID: 1811941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. AU - Herrera,P L, AU - Huarte,J, AU - Sanvito,F, AU - Meda,P, AU - Orci,L, AU - Vassalli,J D, PY - 1991/12/1/pubmed PY - 1991/12/1/medline PY - 1991/12/1/entrez SP - 1257 EP - 65 JF - Development (Cambridge, England) JO - Development VL - 113 IS - 4 N2 - By immunofluorescence on cytospin preparations and on semithin sections of mouse pancreatic buds, we have found glucagon and pancreatic polypeptide (PP)-containing cells at embryonal day 10.5 (E 10.5) in dorsal buds and at E 11.5 in ventral buds. Insulin-containing cells appear in dorsal buds at E 11.5, and one to two days later in ventral buds. Somatostatin-containing cells are detectable from E 13.5 in both dorsal and ventral buds. A quantitative analysis shows that up to E 15.5, PP-containing cells are relatively abundant in both buds. By PCR amplification of oligo(dT)-primed cDNAs prepared from total pancreatic RNA, we also detect PP mRNA from E 10.5 onwards, thus confirming the early expression of the PP gene in the developing mouse pancreas. Analysis of endocrine cells in situ suggests three major patterns of cell distribution in embryonic pancreas. First, individual hormone-containing cells are located within the epithelium of pancreatic ducts. In both dorsal and ventral buds, the majority of these endocrine cells contain PP, but many also contain glucagon, insulin or somatostatin. Secondly, clusters of endocrine cells are found in the pancreatic interstitium. Many of these cells contain both glucagon and PP which, by immunogold labelling of consecutive thin sections, can be shown to co-exist within individual secretory granules. Finally, starting on E 18.5, typical islets are formed with centrally located B cells and with the adult 'one cell-one hormone' phenotype. These results suggest an intriguing ontogenic relationship between A- and PP-cells, and also indicate that PP-containing cells may occupy a hitherto unexpected place in the lineage of endocrine islet cells. SN - 0950-1991 UR - https://www.unboundmedicine.com/medline/citation/1811941/Embryogenesis_of_the_murine_endocrine_pancreas L2 - http://www.komp.org/ncbi.php?PMID=1811941 DB - PRIME DP - Unbound Medicine ER -