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Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer.
Surgery. 2008 Jan; 143(1):113-25.S

Abstract

BACKGROUND

Human telomerase reverse transcriptase (hTERT), a catalytic subunit of telomerase, is a promising diagnostic candidate for pancreatic cancer. To evaluate the feasibility of immunohistochemistry (IHC) for the diagnosis of pancreatic cancer, hTERT expression and telomerase activity were assayed in pancreatic tissues, ex vivo brushing, and pancreatic juice samples with various pancreatic diseases.

METHODS

Telomerase activity was analyzed using the TRAP assay and hTERT was examined by IHC in 85 pancreatic tumor samples, 17 ex vivo pancreatic duct brushings, and 27 pancreatic juice samples.

RESULTS

In tissue samples, telomerase activity was positive in 83% of invasive ductal adenocarcinomas (IDCs) when the specificity was set at 100%, while hTERT was highly expressed in 88% of IDCs. In intraductal papillary mucinous neoplasms (IPMNs), the levels of telomerase activity increased gradually during progression, while hTERT expression was detectable in 8 of 8 malignant IPMNs and 1 of 2 borderline IPMNs. In pancreatic juice samples, 10 of 11 IDCs and 3 of 4 malignant IPMNs expressed hTERT, in which seven samples were not diagnosed as malignant on cytologic exam. The diagnoses of pancreatic cancer based on hTERT IHC exhibited high rates of sensitivity (87%), specificity (92%), and overall accuracy (89%), whereas the sensitivity of cytologic examination was 53%. The additional assessment of hTERT expression and telomerase activity could improve the sensitivity up to 100%.

CONCLUSIONS

Our results suggested that hTERT expression in epithelia indicates malignant transformation in pancreatic tumors and immunohistochemical detection of hTERT in cells derived from pancreatic juice provides a potent method for cancer diagnosis.

Authors+Show Affiliations

Department of Surgery, Division of Clinical Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Japan. hashimoto@hiroshima-u.ac.jp <hashimoto@hiroshima-u.ac.jp>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18154939

Citation

Hashimoto, Yasushi, et al. "Detection of Human Telomerase Reverse Transcriptase (hTERT) Expression in Tissue and Pancreatic Juice From Pancreatic Cancer." Surgery, vol. 143, no. 1, 2008, pp. 113-25.
Hashimoto Y, Murakami Y, Uemura K, et al. Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer. Surgery. 2008;143(1):113-25.
Hashimoto, Y., Murakami, Y., Uemura, K., Hayashidani, Y., Sudo, T., Ohge, H., Fukuda, E., Sueda, T., & Hiyama, E. (2008). Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer. Surgery, 143(1), 113-25.
Hashimoto Y, et al. Detection of Human Telomerase Reverse Transcriptase (hTERT) Expression in Tissue and Pancreatic Juice From Pancreatic Cancer. Surgery. 2008;143(1):113-25. PubMed PMID: 18154939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of human telomerase reverse transcriptase (hTERT) expression in tissue and pancreatic juice from pancreatic cancer. AU - Hashimoto,Yasushi, AU - Murakami,Yoshiaki, AU - Uemura,Kenichiro, AU - Hayashidani,Yasuo, AU - Sudo,Takeshi, AU - Ohge,Hiroki, AU - Fukuda,Emi, AU - Sueda,Taijiro, AU - Hiyama,Eiso, PY - 2007/02/26/received PY - 2007/06/21/revised PY - 2007/07/05/accepted PY - 2007/12/25/pubmed PY - 2008/1/10/medline PY - 2007/12/25/entrez SP - 113 EP - 25 JF - Surgery JO - Surgery VL - 143 IS - 1 N2 - BACKGROUND: Human telomerase reverse transcriptase (hTERT), a catalytic subunit of telomerase, is a promising diagnostic candidate for pancreatic cancer. To evaluate the feasibility of immunohistochemistry (IHC) for the diagnosis of pancreatic cancer, hTERT expression and telomerase activity were assayed in pancreatic tissues, ex vivo brushing, and pancreatic juice samples with various pancreatic diseases. METHODS: Telomerase activity was analyzed using the TRAP assay and hTERT was examined by IHC in 85 pancreatic tumor samples, 17 ex vivo pancreatic duct brushings, and 27 pancreatic juice samples. RESULTS: In tissue samples, telomerase activity was positive in 83% of invasive ductal adenocarcinomas (IDCs) when the specificity was set at 100%, while hTERT was highly expressed in 88% of IDCs. In intraductal papillary mucinous neoplasms (IPMNs), the levels of telomerase activity increased gradually during progression, while hTERT expression was detectable in 8 of 8 malignant IPMNs and 1 of 2 borderline IPMNs. In pancreatic juice samples, 10 of 11 IDCs and 3 of 4 malignant IPMNs expressed hTERT, in which seven samples were not diagnosed as malignant on cytologic exam. The diagnoses of pancreatic cancer based on hTERT IHC exhibited high rates of sensitivity (87%), specificity (92%), and overall accuracy (89%), whereas the sensitivity of cytologic examination was 53%. The additional assessment of hTERT expression and telomerase activity could improve the sensitivity up to 100%. CONCLUSIONS: Our results suggested that hTERT expression in epithelia indicates malignant transformation in pancreatic tumors and immunohistochemical detection of hTERT in cells derived from pancreatic juice provides a potent method for cancer diagnosis. SN - 1532-7361 UR - https://www.unboundmedicine.com/medline/citation/18154939/Detection_of_human_telomerase_reverse_transcriptase__hTERT__expression_in_tissue_and_pancreatic_juice_from_pancreatic_cancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-6060(07)00626-5 DB - PRIME DP - Unbound Medicine ER -