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Determination of volatile organic acids in oriental tobacco by needle-based derivatization headspace liquid-phase microextraction coupled to gas chromatography/mass spectrometry.
J Chromatogr A. 2008 Feb 01; 1179(2):89-95.JC

Abstract

A method coupling needle-based derivatization headspace liquid-phase microextraction with gas chromatography-mass spectrometry (HS-LPME/GC-MS) was developed to determine volatile organic acids in tobacco. The mixture of N,O-bis(trimethylsilyl)trifluoroacetamide and decane was utilized as the solvent for HS-LPME, resulting that extraction and derivatization were simultaneously completed in one step. The solvent served two purposes. First, it pre-concentrated volatile organic acids in the headspace of tobacco sample. Second, the volatile organic acids extracted were derivatized to form silyl derivatives in the drop. The main parameters affecting needle-based derivatization HS-LPME procedure such as extraction and derivatization reagent, microdrop volume, extraction and derivatization time, and preheating temperature and preheating time were optimized. The standard addition approach was essential to obtain accurate measurements by minimizing matrix effects. Good linearity (R(2)> or =0.9804) and good repeatability (RSDs< or =15.3%, n=5) for 16 analytes in spiked standard analytes sample were achieved. The method has the additional advantages that at the same time it is simple, fast, effective, sensitive, selective, and provides an overall profile of volatile organic acids in the oriental tobacco. This paper does offer an alternative approach to determine volatile organic acids in tobacco.

Authors+Show Affiliations

ZhengZhou Tobacco Research Institute, China National Tobacco Corporation, ZhengZhou 450001, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

18155225

Citation

Sun, Shi-Hao, et al. "Determination of Volatile Organic Acids in Oriental Tobacco By Needle-based Derivatization Headspace Liquid-phase Microextraction Coupled to Gas Chromatography/mass Spectrometry." Journal of Chromatography. A, vol. 1179, no. 2, 2008, pp. 89-95.
Sun SH, Xie JP, Xie FW, et al. Determination of volatile organic acids in oriental tobacco by needle-based derivatization headspace liquid-phase microextraction coupled to gas chromatography/mass spectrometry. J Chromatogr A. 2008;1179(2):89-95.
Sun, S. H., Xie, J. P., Xie, F. W., & Zong, Y. L. (2008). Determination of volatile organic acids in oriental tobacco by needle-based derivatization headspace liquid-phase microextraction coupled to gas chromatography/mass spectrometry. Journal of Chromatography. A, 1179(2), 89-95.
Sun SH, et al. Determination of Volatile Organic Acids in Oriental Tobacco By Needle-based Derivatization Headspace Liquid-phase Microextraction Coupled to Gas Chromatography/mass Spectrometry. J Chromatogr A. 2008 Feb 1;1179(2):89-95. PubMed PMID: 18155225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of volatile organic acids in oriental tobacco by needle-based derivatization headspace liquid-phase microextraction coupled to gas chromatography/mass spectrometry. AU - Sun,Shi-Hao, AU - Xie,Jian-Ping, AU - Xie,Fu-Wei, AU - Zong,Yong-Li, Y1 - 2007/12/05/ PY - 2007/10/19/received PY - 2007/11/26/revised PY - 2007/11/28/accepted PY - 2007/12/25/pubmed PY - 2008/3/26/medline PY - 2007/12/25/entrez SP - 89 EP - 95 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1179 IS - 2 N2 - A method coupling needle-based derivatization headspace liquid-phase microextraction with gas chromatography-mass spectrometry (HS-LPME/GC-MS) was developed to determine volatile organic acids in tobacco. The mixture of N,O-bis(trimethylsilyl)trifluoroacetamide and decane was utilized as the solvent for HS-LPME, resulting that extraction and derivatization were simultaneously completed in one step. The solvent served two purposes. First, it pre-concentrated volatile organic acids in the headspace of tobacco sample. Second, the volatile organic acids extracted were derivatized to form silyl derivatives in the drop. The main parameters affecting needle-based derivatization HS-LPME procedure such as extraction and derivatization reagent, microdrop volume, extraction and derivatization time, and preheating temperature and preheating time were optimized. The standard addition approach was essential to obtain accurate measurements by minimizing matrix effects. Good linearity (R(2)> or =0.9804) and good repeatability (RSDs< or =15.3%, n=5) for 16 analytes in spiked standard analytes sample were achieved. The method has the additional advantages that at the same time it is simple, fast, effective, sensitive, selective, and provides an overall profile of volatile organic acids in the oriental tobacco. This paper does offer an alternative approach to determine volatile organic acids in tobacco. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18155225/Determination_of_volatile_organic_acids_in_oriental_tobacco_by_needle_based_derivatization_headspace_liquid_phase_microextraction_coupled_to_gas_chromatography/mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)02094-8 DB - PRIME DP - Unbound Medicine ER -