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Mapping of a major stripe rust resistance gene in Chinese native wheat variety Chike using microsatellite markers.
J Genet Genomics. 2007 Dec; 34(12):1123-30.JG

Abstract

Chike (accession number Su1900), a Chinese native wheat (Triticum aestivum L.) variety, is resistant to the currently prevailing physiological races of Puccinia striiformis Westend. f. sp. tritici in China. Genetic analysis indicated that resistance to the physiological race CY32 of the pathogen in the variety was controlled by one dominant gene. In this study, BSA (bulked segregant analysis) methods and SSRs (simple sequence repeats) marker polymorphic analysis are used to map the gene. The resistant and susceptible DNA bulks were prepared from the segregating F2 population of the cross between Taichung 29, a susceptible variety as maternal parent, and Chike as paternal parent. Over 400 SSR primers were screened, and five SSR markers Xwmc44, Xgwm259, Xwmc367, Xcfa2292, and Xbarc80 on the chromosome arm 1BL were found to be polymorphic between the resistant and the susceptible DNA bulks as well as their parents. Genetic linkage was tested on segregating F2 population with 200 plants, including 140 resistant and 60 susceptible plants. All the five SSR markers were linked to the stripe rust resistance gene in Chike. The genetic distances for the markers Xwmc44, Xgwm259, Xwmc367, Xcfa2292, and Xbarc80 to the target gene were 8.3 cM, 9.1 cM, 17.2 cM, 20.6 cM, and 31.6 cM, respectively. Analysis using 21 nulli-tetrasomic Chinese Spring lines further confirmed that all the five markers were located on chromosome 1B. On the basis of the above results, it is reasonable to assume that the major stripe rust resistance gene YrChk in Chike was located on the chromosome arm 1BL, and its comparison with the other stripe rust resistance genes located on 1B suggested that YrChk may be a novel gene that provides the resistance against stripe rust in Chike. Exploration and utilization of resources of disease resistance genes in native wheat varieties will be helpful both to diversify the resistance genes and to amend the situation of resistance gene simplification in the commercial wheat cultivars in China.

Authors+Show Affiliations

Liu F
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Plant Protection Academy, Agricultural University of Anhui, Hefei 230036, China.
Niu Y
No affiliation info available
Deng H
No affiliation info available
Tan G
No affiliation info available

MeSH

BasidiomycotaBreedingChinaChromosome MappingGenes, PlantGenetic LinkageGenetic MarkersImmunity, InnateMicrosatellite RepeatsPlant DiseasesPolymorphism, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18155625

Citation

Liu, Fanghui, et al. "Mapping of a Major Stripe Rust Resistance Gene in Chinese Native Wheat Variety Chike Using Microsatellite Markers." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 34, no. 12, 2007, pp. 1123-30.
Liu F, Niu Y, Deng H, et al. Mapping of a major stripe rust resistance gene in Chinese native wheat variety Chike using microsatellite markers. J Genet Genomics. 2007;34(12):1123-30.
Liu, F., Niu, Y., Deng, H., & Tan, G. (2007). Mapping of a major stripe rust resistance gene in Chinese native wheat variety Chike using microsatellite markers. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 34(12), 1123-30.
Liu F, et al. Mapping of a Major Stripe Rust Resistance Gene in Chinese Native Wheat Variety Chike Using Microsatellite Markers. J Genet Genomics. 2007;34(12):1123-30. PubMed PMID: 18155625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping of a major stripe rust resistance gene in Chinese native wheat variety Chike using microsatellite markers. AU - Liu,Fanghui, AU - Niu,Yongchun, AU - Deng,Hui, AU - Tan,Genjia, PY - 2007/04/17/received PY - 2007/05/31/revised PY - 2007/12/25/pubmed PY - 2009/4/25/medline PY - 2007/12/25/entrez SP - 1123 EP - 30 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 34 IS - 12 N2 - Chike (accession number Su1900), a Chinese native wheat (Triticum aestivum L.) variety, is resistant to the currently prevailing physiological races of Puccinia striiformis Westend. f. sp. tritici in China. Genetic analysis indicated that resistance to the physiological race CY32 of the pathogen in the variety was controlled by one dominant gene. In this study, BSA (bulked segregant analysis) methods and SSRs (simple sequence repeats) marker polymorphic analysis are used to map the gene. The resistant and susceptible DNA bulks were prepared from the segregating F2 population of the cross between Taichung 29, a susceptible variety as maternal parent, and Chike as paternal parent. Over 400 SSR primers were screened, and five SSR markers Xwmc44, Xgwm259, Xwmc367, Xcfa2292, and Xbarc80 on the chromosome arm 1BL were found to be polymorphic between the resistant and the susceptible DNA bulks as well as their parents. Genetic linkage was tested on segregating F2 population with 200 plants, including 140 resistant and 60 susceptible plants. All the five SSR markers were linked to the stripe rust resistance gene in Chike. The genetic distances for the markers Xwmc44, Xgwm259, Xwmc367, Xcfa2292, and Xbarc80 to the target gene were 8.3 cM, 9.1 cM, 17.2 cM, 20.6 cM, and 31.6 cM, respectively. Analysis using 21 nulli-tetrasomic Chinese Spring lines further confirmed that all the five markers were located on chromosome 1B. On the basis of the above results, it is reasonable to assume that the major stripe rust resistance gene YrChk in Chike was located on the chromosome arm 1BL, and its comparison with the other stripe rust resistance genes located on 1B suggested that YrChk may be a novel gene that provides the resistance against stripe rust in Chike. Exploration and utilization of resources of disease resistance genes in native wheat varieties will be helpful both to diversify the resistance genes and to amend the situation of resistance gene simplification in the commercial wheat cultivars in China. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/18155625/Mapping_of_a_major_stripe_rust_resistance_gene_in_Chinese_native_wheat_variety_Chike_using_microsatellite_markers_ DB - PRIME DP - Unbound Medicine ER -