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Activation of the neurokinin-1 receptor by substance P triggers the release of substance P from cultured adult rat dorsal root ganglion neurons.
Mol Pain. 2007 Dec 25; 3:42.MP

Abstract

BACKGROUND

Although substance P (SP) is an important primary afferent modulator in nociceptive processes, it is unclear whether SP regulates its own release from primary sensory neurons.

RESULTS

Using a highly sensitive radioimmunoassay for SP, we have demonstrated that the activation of neurokinin-1 receptor by SP or GR73632 (a potent neurokinin-1 receptor agonist) triggered an increase of SP release from cultured adult rat dorsal root ganglion (DRG) neurons depending on the dose and exposure time within 60 min, and thereafter, the SP release level gradually decreased over 360 min. Accompanying the SP release, a significant reduction in the percentage of neurons expressing neurokinin-1 receptor on their membranes during exposure to SP (200 pg/dish) occurred time dependently (56 +/- 5% and 32 +/- 2% at 180 and 360 min, respectively). The GR73632-evoked (10 nM, 60 min) SP release was attenuated by several inhibitors for mitogen-activated protein kinase kinase, p38 mitogen-activated protein (MAP) kinase and cyclooxygenase-2 (COX-2), protein kinase C (PKC), respectively. In contrast, a c-Jun NH2-terminal kinase inhibitor increased the GR73632-evoked SP release.

CONCLUSION

These results indicate that the neurokinin-1 receptor activation by its agonists regulates the SP release process involving the activation of MAP kinases, PKCs and COX-2 from cultured DRG neurons.

Authors+Show Affiliations

Department of Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan. hbtang2006@hiroshima-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18157919

Citation

Tang, He-Bin, et al. "Activation of the Neurokinin-1 Receptor By Substance P Triggers the Release of Substance P From Cultured Adult Rat Dorsal Root Ganglion Neurons." Molecular Pain, vol. 3, 2007, p. 42.
Tang HB, Li YS, Arihiro K, et al. Activation of the neurokinin-1 receptor by substance P triggers the release of substance P from cultured adult rat dorsal root ganglion neurons. Mol Pain. 2007;3:42.
Tang, H. B., Li, Y. S., Arihiro, K., & Nakata, Y. (2007). Activation of the neurokinin-1 receptor by substance P triggers the release of substance P from cultured adult rat dorsal root ganglion neurons. Molecular Pain, 3, 42.
Tang HB, et al. Activation of the Neurokinin-1 Receptor By Substance P Triggers the Release of Substance P From Cultured Adult Rat Dorsal Root Ganglion Neurons. Mol Pain. 2007 Dec 25;3:42. PubMed PMID: 18157919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of the neurokinin-1 receptor by substance P triggers the release of substance P from cultured adult rat dorsal root ganglion neurons. AU - Tang,He-Bin, AU - Li,Yu-Sang, AU - Arihiro,Koji, AU - Nakata,Yoshihiro, Y1 - 2007/12/25/ PY - 2007/08/23/received PY - 2007/12/25/accepted PY - 2007/12/26/pubmed PY - 2008/3/20/medline PY - 2007/12/26/entrez SP - 42 EP - 42 JF - Molecular pain JO - Mol Pain VL - 3 N2 - BACKGROUND: Although substance P (SP) is an important primary afferent modulator in nociceptive processes, it is unclear whether SP regulates its own release from primary sensory neurons. RESULTS: Using a highly sensitive radioimmunoassay for SP, we have demonstrated that the activation of neurokinin-1 receptor by SP or GR73632 (a potent neurokinin-1 receptor agonist) triggered an increase of SP release from cultured adult rat dorsal root ganglion (DRG) neurons depending on the dose and exposure time within 60 min, and thereafter, the SP release level gradually decreased over 360 min. Accompanying the SP release, a significant reduction in the percentage of neurons expressing neurokinin-1 receptor on their membranes during exposure to SP (200 pg/dish) occurred time dependently (56 +/- 5% and 32 +/- 2% at 180 and 360 min, respectively). The GR73632-evoked (10 nM, 60 min) SP release was attenuated by several inhibitors for mitogen-activated protein kinase kinase, p38 mitogen-activated protein (MAP) kinase and cyclooxygenase-2 (COX-2), protein kinase C (PKC), respectively. In contrast, a c-Jun NH2-terminal kinase inhibitor increased the GR73632-evoked SP release. CONCLUSION: These results indicate that the neurokinin-1 receptor activation by its agonists regulates the SP release process involving the activation of MAP kinases, PKCs and COX-2 from cultured DRG neurons. SN - 1744-8069 UR - https://www.unboundmedicine.com/medline/citation/18157919/Activation_of_the_neurokinin_1_receptor_by_substance_P_triggers_the_release_of_substance_P_from_cultured_adult_rat_dorsal_root_ganglion_neurons_ L2 - https://journals.sagepub.com/doi/10.1186/1744-8069-3-42?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -