[Determination of the molecular mass of modified citrus pectin using high performance size exclusion chromatography].Se Pu. 2007 Sep; 25(5):711-4.SP
Modified citrus pectin (MCP) is a polysaccharide with anti-tumor growth, anti-invasiveness and anti-metastasis activities. It is of significance to determine the molecular mass and polydispersity of polysaccharide, which could influence pharmacological activities of polysaccharide. To the best of our knowledge, no study has been reported on calculating the molecular mass of MCP by high performance size exclusion chromatography-refractive index detection (HPSEC-RID). A TSK G3000PW(XL) column was used at 40 degrees C, and the mobile phase was composed of 50 mmol/L NH4Ac solution. Pullulan with known molecular mass was chosen as calibration standard to obtain regression equation. Relative molecular mass of MCP can be derived from the regression equation. Relative molecular masses of MCP were obtained: M(n), 21000; M(w), 43000; M(p), 46000; M(z), 66000. The polydispersity index of MCP was 2. The established method is simple, rapid and repeatable for quality control in preparing MCP and it would be also applicable to the study of composition-activity relationship of MCP in the future.