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[Determination of the molecular mass of modified citrus pectin using high performance size exclusion chromatography].
Se Pu. 2007 Sep; 25(5):711-4.SP

Abstract

Modified citrus pectin (MCP) is a polysaccharide with anti-tumor growth, anti-invasiveness and anti-metastasis activities. It is of significance to determine the molecular mass and polydispersity of polysaccharide, which could influence pharmacological activities of polysaccharide. To the best of our knowledge, no study has been reported on calculating the molecular mass of MCP by high performance size exclusion chromatography-refractive index detection (HPSEC-RID). A TSK G3000PW(XL) column was used at 40 degrees C, and the mobile phase was composed of 50 mmol/L NH4Ac solution. Pullulan with known molecular mass was chosen as calibration standard to obtain regression equation. Relative molecular mass of MCP can be derived from the regression equation. Relative molecular masses of MCP were obtained: M(n), 21000; M(w), 43000; M(p), 46000; M(z), 66000. The polydispersity index of MCP was 2. The established method is simple, rapid and repeatable for quality control in preparing MCP and it would be also applicable to the study of composition-activity relationship of MCP in the future.

Authors+Show Affiliations

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. zhwenbo@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

18161323

Citation

Zhang, Wenbo, et al. "[Determination of the Molecular Mass of Modified Citrus Pectin Using High Performance Size Exclusion Chromatography]." Se Pu = Chinese Journal of Chromatography, vol. 25, no. 5, 2007, pp. 711-4.
Zhang W, Gao L, Shi X, et al. [Determination of the molecular mass of modified citrus pectin using high performance size exclusion chromatography]. Se Pu. 2007;25(5):711-4.
Zhang, W., Gao, L., Shi, X., & Zhang, Q. (2007). [Determination of the molecular mass of modified citrus pectin using high performance size exclusion chromatography]. Se Pu = Chinese Journal of Chromatography, 25(5), 711-4.
Zhang W, et al. [Determination of the Molecular Mass of Modified Citrus Pectin Using High Performance Size Exclusion Chromatography]. Se Pu. 2007;25(5):711-4. PubMed PMID: 18161323.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of the molecular mass of modified citrus pectin using high performance size exclusion chromatography]. AU - Zhang,Wenbo, AU - Gao,Lin, AU - Shi,Xiufang, AU - Zhang,Qilai, PY - 2007/12/29/pubmed PY - 2010/6/18/medline PY - 2007/12/29/entrez SP - 711 EP - 4 JF - Se pu = Chinese journal of chromatography JO - Se Pu VL - 25 IS - 5 N2 - Modified citrus pectin (MCP) is a polysaccharide with anti-tumor growth, anti-invasiveness and anti-metastasis activities. It is of significance to determine the molecular mass and polydispersity of polysaccharide, which could influence pharmacological activities of polysaccharide. To the best of our knowledge, no study has been reported on calculating the molecular mass of MCP by high performance size exclusion chromatography-refractive index detection (HPSEC-RID). A TSK G3000PW(XL) column was used at 40 degrees C, and the mobile phase was composed of 50 mmol/L NH4Ac solution. Pullulan with known molecular mass was chosen as calibration standard to obtain regression equation. Relative molecular mass of MCP can be derived from the regression equation. Relative molecular masses of MCP were obtained: M(n), 21000; M(w), 43000; M(p), 46000; M(z), 66000. The polydispersity index of MCP was 2. The established method is simple, rapid and repeatable for quality control in preparing MCP and it would be also applicable to the study of composition-activity relationship of MCP in the future. SN - 1000-8713 UR - https://www.unboundmedicine.com/medline/citation/18161323/[Determination_of_the_molecular_mass_of_modified_citrus_pectin_using_high_performance_size_exclusion_chromatography]_ DB - PRIME DP - Unbound Medicine ER -
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