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Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical validation.
Osteoarthritis Cartilage. 2008 Jul; 16(7):779-86.OC

Abstract

OBJECTIVES

Validate use of a commercially available immunoassay for measurement of bone alkaline phosphatase (BAP) in equine serum and synovial fluid (SF), and investigate the effects of osteochondral (OC) injury in horses on BAP concentrations in serum and SF.

METHODS

SF was collected from 37 joints of 34 Thoroughbred (TB) racehorses undergoing arthroscopic surgery for the removal of OC fragments from either the carpal joints (n=18) or the metacarpo-/metatarsophalangeal (MP) joints (n=19). SF was also obtained from 52 joints of 16 normal TB horses, collected bilaterally from carpal joints of 10 horses (n=40), and MP joints of six horses (n=12). Blood was obtained from all 50 horses. A commercially available immunoassay was validated and subsequently used to determine equine serum and SF BAP concentrations. Correlations to radiographic and arthroscopic scores were assessed.

RESULTS

BAP concentrations were significantly lower in serum from horses with OC injury in their carpal or MP joints than in serum from normal horses. SF BAP concentrations in normal and OC injured carpal joints were significantly higher than MP joints. BAP concentrations were significantly higher in SF from OC injured carpal joints than normal. BAP concentrations were affected by joint sampled, with age having a significant interaction. Concentrations of BAP in the serum (<30U/L), SF (>22U/L) and a ratio of SF to serum > or = 0.5 were predictive of OC injury. Radiographic and arthroscopic scores significantly correlated with serum BAP concentrations, and SF:serum BAP correlated with arthroscopic scores.

CONCLUSIONS

Determination of serum and SF BAP concentrations may be beneficial in the investigation of early joint injury. Joint and injury dependent differences in BAP concentrations allowed the estimation of predictive value for identifying OC injury.

Authors+Show Affiliations

Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-1432, USA. tumb016@umn.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

18162418

Citation

Trumble, T N., et al. "Joint Dependent Concentrations of Bone Alkaline Phosphatase in Serum and Synovial Fluids of Horses With Osteochondral Injury: an Analytical and Clinical Validation." Osteoarthritis and Cartilage, vol. 16, no. 7, 2008, pp. 779-86.
Trumble TN, Brown MP, Merritt KA, et al. Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical validation. Osteoarthr Cartil. 2008;16(7):779-86.
Trumble, T. N., Brown, M. P., Merritt, K. A., & Billinghurst, R. C. (2008). Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical validation. Osteoarthritis and Cartilage, 16(7), 779-86.
Trumble TN, et al. Joint Dependent Concentrations of Bone Alkaline Phosphatase in Serum and Synovial Fluids of Horses With Osteochondral Injury: an Analytical and Clinical Validation. Osteoarthr Cartil. 2008;16(7):779-86. PubMed PMID: 18162418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical validation. AU - Trumble,T N, AU - Brown,M P, AU - Merritt,K A, AU - Billinghurst,R C, Y1 - 2007/12/26/ PY - 2007/08/14/received PY - 2007/11/17/accepted PY - 2007/12/29/pubmed PY - 2008/12/17/medline PY - 2007/12/29/entrez SP - 779 EP - 86 JF - Osteoarthritis and cartilage JO - Osteoarthr. Cartil. VL - 16 IS - 7 N2 - OBJECTIVES: Validate use of a commercially available immunoassay for measurement of bone alkaline phosphatase (BAP) in equine serum and synovial fluid (SF), and investigate the effects of osteochondral (OC) injury in horses on BAP concentrations in serum and SF. METHODS: SF was collected from 37 joints of 34 Thoroughbred (TB) racehorses undergoing arthroscopic surgery for the removal of OC fragments from either the carpal joints (n=18) or the metacarpo-/metatarsophalangeal (MP) joints (n=19). SF was also obtained from 52 joints of 16 normal TB horses, collected bilaterally from carpal joints of 10 horses (n=40), and MP joints of six horses (n=12). Blood was obtained from all 50 horses. A commercially available immunoassay was validated and subsequently used to determine equine serum and SF BAP concentrations. Correlations to radiographic and arthroscopic scores were assessed. RESULTS: BAP concentrations were significantly lower in serum from horses with OC injury in their carpal or MP joints than in serum from normal horses. SF BAP concentrations in normal and OC injured carpal joints were significantly higher than MP joints. BAP concentrations were significantly higher in SF from OC injured carpal joints than normal. BAP concentrations were affected by joint sampled, with age having a significant interaction. Concentrations of BAP in the serum (<30U/L), SF (>22U/L) and a ratio of SF to serum > or = 0.5 were predictive of OC injury. Radiographic and arthroscopic scores significantly correlated with serum BAP concentrations, and SF:serum BAP correlated with arthroscopic scores. CONCLUSIONS: Determination of serum and SF BAP concentrations may be beneficial in the investigation of early joint injury. Joint and injury dependent differences in BAP concentrations allowed the estimation of predictive value for identifying OC injury. SN - 1063-4584 UR - https://www.unboundmedicine.com/medline/citation/18162418/Joint_dependent_concentrations_of_bone_alkaline_phosphatase_in_serum_and_synovial_fluids_of_horses_with_osteochondral_injury:_an_analytical_and_clinical_validation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1063-4584(07)00375-5 DB - PRIME DP - Unbound Medicine ER -