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The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection.
Pediatr Infect Dis J. 2008 Jan; 27(1):43-8.PI

Abstract

BACKGROUND

To evaluate the use of dengue nonstructural protein 1 (NS1) antigen for the early diagnosis during the febrile stage in patients with dengue infection.

METHODS

A total of 445 sera obtained from 165 patients [dengue fever (DF): 42, dengue hemorrhagic fever (DHF) grade I: 50, II: 63, III and IV: 10] and 8 other febrile illnesses 5-15 years of age, were assayed for the NS1 antigen, dengue-specific Ig M and Ig G antibodies.

RESULTS

The positive rates of NS1 antigen among patients with either DF or DHF was 100% (7 of 7) on day 2, 92.3% (12 of 13) on day 3, 76.9% (40 of 52) on day 4, 56.5% (61 of 108) on day 5 of fever; and declined to 43.1% (59 of 137) on day 6 with defervescence and 29.8% (25 of 84) on day 7 (1 day after defervescence). The positive rates of patients with DF were higher than those with DHF but no statistically significant difference was found. However, patients with primary DHF infection had significantly higher positive rates than those with secondary DHF infection. The positive rates of Ig M antibodies were in reverse proportion to those of NS1 antigen. The additional Ig M antibody determination increased the positive rates to 90.4% (47 of 52) on day 4, 83.3% (90 of 108) on day 5 of fever; 95.6% (131 of 137) on day 6 with defervescence, and 88.1% (74 of 84) on day 7.

CONCLUSIONS

Dengue NS1 antigen testing is suggested as a helpful tool for the early diagnosis of dengue infection after the onset of fever. The additional Ig M antibody determination increased the diagnostic rates.

Authors+Show Affiliations

Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Bangkok, Thailand. raajs@mahidol.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18162937

Citation

Chuansumrit, Ampaiwan, et al. "The Use of Dengue Nonstructural Protein 1 Antigen for the Early Diagnosis During the Febrile Stage in Patients With Dengue Infection." The Pediatric Infectious Disease Journal, vol. 27, no. 1, 2008, pp. 43-8.
Chuansumrit A, Chaiyaratana W, Pongthanapisith V, et al. The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection. Pediatr Infect Dis J. 2008;27(1):43-8.
Chuansumrit, A., Chaiyaratana, W., Pongthanapisith, V., Tangnararatchakit, K., Lertwongrath, S., & Yoksan, S. (2008). The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection. The Pediatric Infectious Disease Journal, 27(1), 43-8.
Chuansumrit A, et al. The Use of Dengue Nonstructural Protein 1 Antigen for the Early Diagnosis During the Febrile Stage in Patients With Dengue Infection. Pediatr Infect Dis J. 2008;27(1):43-8. PubMed PMID: 18162937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection. AU - Chuansumrit,Ampaiwan, AU - Chaiyaratana,Wathanee, AU - Pongthanapisith,Viroj, AU - Tangnararatchakit,Kanchana, AU - Lertwongrath,Sarapee, AU - Yoksan,Sutee, PY - 2007/12/29/pubmed PY - 2008/1/30/medline PY - 2007/12/29/entrez SP - 43 EP - 8 JF - The Pediatric infectious disease journal JO - Pediatr Infect Dis J VL - 27 IS - 1 N2 - BACKGROUND: To evaluate the use of dengue nonstructural protein 1 (NS1) antigen for the early diagnosis during the febrile stage in patients with dengue infection. METHODS: A total of 445 sera obtained from 165 patients [dengue fever (DF): 42, dengue hemorrhagic fever (DHF) grade I: 50, II: 63, III and IV: 10] and 8 other febrile illnesses 5-15 years of age, were assayed for the NS1 antigen, dengue-specific Ig M and Ig G antibodies. RESULTS: The positive rates of NS1 antigen among patients with either DF or DHF was 100% (7 of 7) on day 2, 92.3% (12 of 13) on day 3, 76.9% (40 of 52) on day 4, 56.5% (61 of 108) on day 5 of fever; and declined to 43.1% (59 of 137) on day 6 with defervescence and 29.8% (25 of 84) on day 7 (1 day after defervescence). The positive rates of patients with DF were higher than those with DHF but no statistically significant difference was found. However, patients with primary DHF infection had significantly higher positive rates than those with secondary DHF infection. The positive rates of Ig M antibodies were in reverse proportion to those of NS1 antigen. The additional Ig M antibody determination increased the positive rates to 90.4% (47 of 52) on day 4, 83.3% (90 of 108) on day 5 of fever; 95.6% (131 of 137) on day 6 with defervescence, and 88.1% (74 of 84) on day 7. CONCLUSIONS: Dengue NS1 antigen testing is suggested as a helpful tool for the early diagnosis of dengue infection after the onset of fever. The additional Ig M antibody determination increased the diagnostic rates. SN - 0891-3668 UR - https://www.unboundmedicine.com/medline/citation/18162937/The_use_of_dengue_nonstructural_protein_1_antigen_for_the_early_diagnosis_during_the_febrile_stage_in_patients_with_dengue_infection_ L2 - https://doi.org/10.1097/INF.0b013e318150666d DB - PRIME DP - Unbound Medicine ER -