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Decorin enhances the proliferation and differentiation of myogenic cells through suppressing myostatin activity.
J Cell Physiol. 2008 Jun; 215(3):856-67.JC

Abstract

Decorin, a small leucine-rich proteoglycan, plays an important role in the regulation of cell growth. Our recent study has shown that immobilized decorin in the collagen matrix sequesters myostatin into the extracellular matrix and prevents its inhibitory action to myoblast proliferation in vitro. However, it still remains unclear whether free decorin could affect the proliferation and differentiation of myogenic cells by regulating myostatin activity. In the present study, we generated stable clonal C2C12 myoblasts that were over-expressing decorin, and showed that decorin over-expressing cells had an increased rate of proliferation as compared to control cells. Decorin over-expressing cells formed multi-giant hypertrophic myotubes with an elongated morphology and larger size as compared to control cells, although the initiation of differentiation in decorin over-expressing cells was somewhat delayed as compared to control cells. Western blot analysis demonstrated that MyoD expression in decorin over-expressing cells was lower than that in control cells until 12 h after induction to differentiate. At 48-h differentiation, the expressions of MyoD, p21 and myogenin were dramatically increased in cells that over-expressed decorin. Furthermore, we revealed that over-expression of decorin suppressed the activity of myostatin endogenously synthesized in C2C12 myoblasts and attenuated the signaling of exogenous myostatin. Consistent with these results, knock-down of decorin impairs C2C12 myoblast growth by increasing the sensitivity to exogenous myostatin. These results clearly show that decorin enhances the proliferation and differentiation of C2C12 myoblasts through suppressing myostatin activity.

Authors+Show Affiliations

Meat Science Laboratory, Division of Bioresources and Bioproduction, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18163379

Citation

Kishioka, Yasuhiro, et al. "Decorin Enhances the Proliferation and Differentiation of Myogenic Cells Through Suppressing Myostatin Activity." Journal of Cellular Physiology, vol. 215, no. 3, 2008, pp. 856-67.
Kishioka Y, Thomas M, Wakamatsu J, et al. Decorin enhances the proliferation and differentiation of myogenic cells through suppressing myostatin activity. J Cell Physiol. 2008;215(3):856-67.
Kishioka, Y., Thomas, M., Wakamatsu, J., Hattori, A., Sharma, M., Kambadur, R., & Nishimura, T. (2008). Decorin enhances the proliferation and differentiation of myogenic cells through suppressing myostatin activity. Journal of Cellular Physiology, 215(3), 856-67. https://doi.org/10.1002/jcp.21371
Kishioka Y, et al. Decorin Enhances the Proliferation and Differentiation of Myogenic Cells Through Suppressing Myostatin Activity. J Cell Physiol. 2008;215(3):856-67. PubMed PMID: 18163379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Decorin enhances the proliferation and differentiation of myogenic cells through suppressing myostatin activity. AU - Kishioka,Yasuhiro, AU - Thomas,Mark, AU - Wakamatsu,Jun-Ichi, AU - Hattori,Akihito, AU - Sharma,Mridula, AU - Kambadur,Ravi, AU - Nishimura,Takanori, PY - 2008/1/1/pubmed PY - 2008/4/30/medline PY - 2008/1/1/entrez SP - 856 EP - 67 JF - Journal of cellular physiology JO - J Cell Physiol VL - 215 IS - 3 N2 - Decorin, a small leucine-rich proteoglycan, plays an important role in the regulation of cell growth. Our recent study has shown that immobilized decorin in the collagen matrix sequesters myostatin into the extracellular matrix and prevents its inhibitory action to myoblast proliferation in vitro. However, it still remains unclear whether free decorin could affect the proliferation and differentiation of myogenic cells by regulating myostatin activity. In the present study, we generated stable clonal C2C12 myoblasts that were over-expressing decorin, and showed that decorin over-expressing cells had an increased rate of proliferation as compared to control cells. Decorin over-expressing cells formed multi-giant hypertrophic myotubes with an elongated morphology and larger size as compared to control cells, although the initiation of differentiation in decorin over-expressing cells was somewhat delayed as compared to control cells. Western blot analysis demonstrated that MyoD expression in decorin over-expressing cells was lower than that in control cells until 12 h after induction to differentiate. At 48-h differentiation, the expressions of MyoD, p21 and myogenin were dramatically increased in cells that over-expressed decorin. Furthermore, we revealed that over-expression of decorin suppressed the activity of myostatin endogenously synthesized in C2C12 myoblasts and attenuated the signaling of exogenous myostatin. Consistent with these results, knock-down of decorin impairs C2C12 myoblast growth by increasing the sensitivity to exogenous myostatin. These results clearly show that decorin enhances the proliferation and differentiation of C2C12 myoblasts through suppressing myostatin activity. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/18163379/Decorin_enhances_the_proliferation_and_differentiation_of_myogenic_cells_through_suppressing_myostatin_activity_ L2 - https://doi.org/10.1002/jcp.21371 DB - PRIME DP - Unbound Medicine ER -