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Microwave-assisted efficient extraction of different parts of Hippophae rhamnoides for the comparative evaluation of antioxidant activity and quantification of its phenolic constituents by reverse-phase high-performance liquid chromatography (RP-HPLC).
J Agric Food Chem. 2008 Jan 23; 56(2):374-9.JA

Abstract

The outcome of different extraction procedures (microwave, ultrasound, Soxhlet, and maceration) on the antioxidant activity of seeds, leaves, pulp, and fruits of Hippophae rhamnoides (sea buckthorn or SBT) was investigated by two different bioassays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The SBT extracts were found to possess strong antioxidant activity measured in terms of TEAC (2.03-182.13 and 6.97-282.75 mg/g) with ABTS and DPPH assays, respectively. In general, the antioxidant capacity of microwave-assisted extracts was found to be significantly higher than those obtained by ultrasound-assisted extraction (UAE) and maceration while being slightly higher than Soxhlet extracts. Further, microwave extracts of seeds were found to possess maximum antioxidant capacity followed by leaves, fruits, and pulp. Also, the chemical composition of extracts, studied in terms of the total phenolic content, was found to be in the range of 1.9-23.5 mg/g Gallic acid equivalent (GAE), which indicates a strong correlation between antioxidant activity and phenolic content present in the SBT. In addition, some of its bioactive phenolic constituents, such as rutin (1), quercetin-3- O-galactoside (2), quercetin (3), myricetin (4), kaempferol (5), and isorhamnetin (6), were also quantified in different extracts by reverse-phase high-performance liquid chromatography (RP-HPLC).

Authors+Show Affiliations

Natural Plant Products, and Hill Area Tea Science Divisions, Institute of Himalayan Bioresource Technology, Post Box 6, Palampur-176061, Himachal Pradesh, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18163559

Citation

Sharma, Upendra K., et al. "Microwave-assisted Efficient Extraction of Different Parts of Hippophae Rhamnoides for the Comparative Evaluation of Antioxidant Activity and Quantification of Its Phenolic Constituents By Reverse-phase High-performance Liquid Chromatography (RP-HPLC)." Journal of Agricultural and Food Chemistry, vol. 56, no. 2, 2008, pp. 374-9.
Sharma UK, Sharma K, Sharma N, et al. Microwave-assisted efficient extraction of different parts of Hippophae rhamnoides for the comparative evaluation of antioxidant activity and quantification of its phenolic constituents by reverse-phase high-performance liquid chromatography (RP-HPLC). J Agric Food Chem. 2008;56(2):374-9.
Sharma, U. K., Sharma, K., Sharma, N., Sharma, A., Singh, H. P., & Sinha, A. K. (2008). Microwave-assisted efficient extraction of different parts of Hippophae rhamnoides for the comparative evaluation of antioxidant activity and quantification of its phenolic constituents by reverse-phase high-performance liquid chromatography (RP-HPLC). Journal of Agricultural and Food Chemistry, 56(2), 374-9. https://doi.org/10.1021/jf072510j
Sharma UK, et al. Microwave-assisted Efficient Extraction of Different Parts of Hippophae Rhamnoides for the Comparative Evaluation of Antioxidant Activity and Quantification of Its Phenolic Constituents By Reverse-phase High-performance Liquid Chromatography (RP-HPLC). J Agric Food Chem. 2008 Jan 23;56(2):374-9. PubMed PMID: 18163559.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microwave-assisted efficient extraction of different parts of Hippophae rhamnoides for the comparative evaluation of antioxidant activity and quantification of its phenolic constituents by reverse-phase high-performance liquid chromatography (RP-HPLC). AU - Sharma,Upendra K, AU - Sharma,Kapil, AU - Sharma,Nandini, AU - Sharma,Abhishek, AU - Singh,Harsh P, AU - Sinha,Arun K, Y1 - 2007/12/29/ PY - 2008/1/1/pubmed PY - 2008/4/9/medline PY - 2008/1/1/entrez SP - 374 EP - 9 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 56 IS - 2 N2 - The outcome of different extraction procedures (microwave, ultrasound, Soxhlet, and maceration) on the antioxidant activity of seeds, leaves, pulp, and fruits of Hippophae rhamnoides (sea buckthorn or SBT) was investigated by two different bioassays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The SBT extracts were found to possess strong antioxidant activity measured in terms of TEAC (2.03-182.13 and 6.97-282.75 mg/g) with ABTS and DPPH assays, respectively. In general, the antioxidant capacity of microwave-assisted extracts was found to be significantly higher than those obtained by ultrasound-assisted extraction (UAE) and maceration while being slightly higher than Soxhlet extracts. Further, microwave extracts of seeds were found to possess maximum antioxidant capacity followed by leaves, fruits, and pulp. Also, the chemical composition of extracts, studied in terms of the total phenolic content, was found to be in the range of 1.9-23.5 mg/g Gallic acid equivalent (GAE), which indicates a strong correlation between antioxidant activity and phenolic content present in the SBT. In addition, some of its bioactive phenolic constituents, such as rutin (1), quercetin-3- O-galactoside (2), quercetin (3), myricetin (4), kaempferol (5), and isorhamnetin (6), were also quantified in different extracts by reverse-phase high-performance liquid chromatography (RP-HPLC). SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/18163559/Microwave_assisted_efficient_extraction_of_different_parts_of_Hippophae_rhamnoides_for_the_comparative_evaluation_of_antioxidant_activity_and_quantification_of_its_phenolic_constituents_by_reverse_phase_high_performance_liquid_chromatography__RP_HPLC__ L2 - https://doi.org/10.1021/jf072510j DB - PRIME DP - Unbound Medicine ER -