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A novel serine protease inhibitor from Bungarus fasciatus venom.
Peptides. 2008 Mar; 29(3):369-74.P

Abstract

By Sephadex G-50 gel filtration, cation-exchange CM-Sephadex C-25 chromatography and reversed phase high-performance liquid chromatography (HPLC), a novel serine protease inhibitor named bungaruskunin was purified and characterized from venom of Bungarus fasciatus. Its cDNA was also cloned from the cDNA library of B. fasciatus venomous glands. The predicted precursor is composed of 83 amino acid (aa) residues including a 24-aa signal peptide and a 59-aa mature bungaruskunin. Bungaruskunin showed maximal similarity (64%) with the predicted serine protease inhibitor blackelin deduced from the cDNA sequence of the red-bellied black snake Pseudechis porphyriacus. Bungaruskunin is a Kunitz protease inhibitor with a conserved Kunitz domain and could exert inhibitory activity against trypsin, chymotrypsin, and elastase. By screening the cDNA library, two new B chains of beta-bungarotoxin are also identified. The overall structures of bungaruskunin and beta-bungarotoxin B chains are similar; especially they have highly conserved signal peptide sequences. These findings strongly suggest that snake Kunitz/BPTI protease inhibitors and neurotoxic homologs may have originated from a common ancestor.

Authors+Show Affiliations

Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18164783

Citation

Lu, Jia, et al. "A Novel Serine Protease Inhibitor From Bungarus Fasciatus Venom." Peptides, vol. 29, no. 3, 2008, pp. 369-74.
Lu J, Yang H, Yu H, et al. A novel serine protease inhibitor from Bungarus fasciatus venom. Peptides. 2008;29(3):369-74.
Lu, J., Yang, H., Yu, H., Gao, W., Lai, R., Liu, J., & Liang, X. (2008). A novel serine protease inhibitor from Bungarus fasciatus venom. Peptides, 29(3), 369-74. https://doi.org/10.1016/j.peptides.2007.11.013
Lu J, et al. A Novel Serine Protease Inhibitor From Bungarus Fasciatus Venom. Peptides. 2008;29(3):369-74. PubMed PMID: 18164783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel serine protease inhibitor from Bungarus fasciatus venom. AU - Lu,Jia, AU - Yang,Hailong, AU - Yu,Haining, AU - Gao,Weikai, AU - Lai,Ren, AU - Liu,Jingze, AU - Liang,Xingcai, Y1 - 2007/11/26/ PY - 2007/10/22/received PY - 2007/11/19/revised PY - 2007/11/19/accepted PY - 2008/1/1/pubmed PY - 2008/7/22/medline PY - 2008/1/1/entrez SP - 369 EP - 74 JF - Peptides JO - Peptides VL - 29 IS - 3 N2 - By Sephadex G-50 gel filtration, cation-exchange CM-Sephadex C-25 chromatography and reversed phase high-performance liquid chromatography (HPLC), a novel serine protease inhibitor named bungaruskunin was purified and characterized from venom of Bungarus fasciatus. Its cDNA was also cloned from the cDNA library of B. fasciatus venomous glands. The predicted precursor is composed of 83 amino acid (aa) residues including a 24-aa signal peptide and a 59-aa mature bungaruskunin. Bungaruskunin showed maximal similarity (64%) with the predicted serine protease inhibitor blackelin deduced from the cDNA sequence of the red-bellied black snake Pseudechis porphyriacus. Bungaruskunin is a Kunitz protease inhibitor with a conserved Kunitz domain and could exert inhibitory activity against trypsin, chymotrypsin, and elastase. By screening the cDNA library, two new B chains of beta-bungarotoxin are also identified. The overall structures of bungaruskunin and beta-bungarotoxin B chains are similar; especially they have highly conserved signal peptide sequences. These findings strongly suggest that snake Kunitz/BPTI protease inhibitors and neurotoxic homologs may have originated from a common ancestor. SN - 0196-9781 UR - https://www.unboundmedicine.com/medline/citation/18164783/A_novel_serine_protease_inhibitor_from_Bungarus_fasciatus_venom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(07)00472-X DB - PRIME DP - Unbound Medicine ER -