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A vaccine prepared from a non-pathogenic H5N1 avian influenza virus strain confers protective immunity against highly pathogenic avian influenza virus infection in cynomolgus macaques.
Vaccine. 2008 Jan 24; 26(4):562-72.V

Abstract

In order to prepare for the emergence of pandemic influenza viruses, we have established an influenza virus library that contains non-pathogenic influenza A virus strains with 135 combinations of 15 hemagglutinin and 9 neuraminidase subtypes. In this study, we developed a vaccine against H5N1 highly pathogenic avian influenza (HPAI) virus infection in humans using a virus strain selected from the library. We examined its immunogenic potency using cynomolgus macaques as a primate model. Virus antigen-specific antibodies were elicited by intranasal or subcutaneous administration of inactivated whole virus particle vaccines. After challenge with an H5N1 HPAI virus isolate obtained from a Vietnamese patient, the virus was detected only on next day following inoculation in the nasal and/or tracheal swabs of vaccinated macaques that were asymptomatic. On the other hand, the viruses were isolated from nasal and tracheal swabs from non-vaccinated macaques until day 5 and day 7 after inoculation of the H5N1 HPAI virus, respectively. Although six non-vaccinated macaques developed a high body temperature, and two of them lost their appetite after HPAI virus infection, they recovered by the end of the 12-day observation period and did not show the severe symptoms that have been reported in human H5N1 virus infection cases. This demonstrates that the vaccine prepared with the non-pathogenic H5N1 virus from our influenza virus library conferred protective immunity against H5N1 HPAI virus infection to macaques.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Itoh Y
Department of Pathology, Shiga University of Medical Science, Otsu, Shiga, Japan.
Ozaki H
No affiliation info available
Tsuchiya H
No affiliation info available
Okamoto K
No affiliation info available
Torii R
No affiliation info available
Sakoda Y
No affiliation info available
Kawaoka Y
No affiliation info available
Ogasawara K
No affiliation info available
Kida H
No affiliation info available

MeSH

Administration, IntranasalAnimalsAntibodies, ViralAntibody SpecificityChick EmbryoHumansInfluenza A Virus, H5N1 SubtypeInfluenza A Virus, H5N2 SubtypeInfluenza A virusInfluenza VaccinesInjections, SubcutaneousMacaca fascicularisOrthomyxoviridae InfectionsVaccinationVaccines, InactivatedVirulence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18164788

Citation

Itoh, Yasushi, et al. "A Vaccine Prepared From a Non-pathogenic H5N1 Avian Influenza Virus Strain Confers Protective Immunity Against Highly Pathogenic Avian Influenza Virus Infection in Cynomolgus Macaques." Vaccine, vol. 26, no. 4, 2008, pp. 562-72.
Itoh Y, Ozaki H, Tsuchiya H, et al. A vaccine prepared from a non-pathogenic H5N1 avian influenza virus strain confers protective immunity against highly pathogenic avian influenza virus infection in cynomolgus macaques. Vaccine. 2008;26(4):562-72.
Itoh, Y., Ozaki, H., Tsuchiya, H., Okamoto, K., Torii, R., Sakoda, Y., Kawaoka, Y., Ogasawara, K., & Kida, H. (2008). A vaccine prepared from a non-pathogenic H5N1 avian influenza virus strain confers protective immunity against highly pathogenic avian influenza virus infection in cynomolgus macaques. Vaccine, 26(4), 562-72. https://doi.org/10.1016/j.vaccine.2007.11.031
Itoh Y, et al. A Vaccine Prepared From a Non-pathogenic H5N1 Avian Influenza Virus Strain Confers Protective Immunity Against Highly Pathogenic Avian Influenza Virus Infection in Cynomolgus Macaques. Vaccine. 2008 Jan 24;26(4):562-72. PubMed PMID: 18164788.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A vaccine prepared from a non-pathogenic H5N1 avian influenza virus strain confers protective immunity against highly pathogenic avian influenza virus infection in cynomolgus macaques. AU - Itoh,Yasushi, AU - Ozaki,Hiroichi, AU - Tsuchiya,Hideaki, AU - Okamoto,Kiyoko, AU - Torii,Ryuzo, AU - Sakoda,Yoshihiro, AU - Kawaoka,Yoshihiro, AU - Ogasawara,Kazumasa, AU - Kida,Hiroshi, Y1 - 2007/12/03/ PY - 2007/05/29/received PY - 2007/09/28/revised PY - 2007/11/04/accepted PY - 2008/1/1/pubmed PY - 2008/4/12/medline PY - 2008/1/1/entrez SP - 562 EP - 72 JF - Vaccine JO - Vaccine VL - 26 IS - 4 N2 - In order to prepare for the emergence of pandemic influenza viruses, we have established an influenza virus library that contains non-pathogenic influenza A virus strains with 135 combinations of 15 hemagglutinin and 9 neuraminidase subtypes. In this study, we developed a vaccine against H5N1 highly pathogenic avian influenza (HPAI) virus infection in humans using a virus strain selected from the library. We examined its immunogenic potency using cynomolgus macaques as a primate model. Virus antigen-specific antibodies were elicited by intranasal or subcutaneous administration of inactivated whole virus particle vaccines. After challenge with an H5N1 HPAI virus isolate obtained from a Vietnamese patient, the virus was detected only on next day following inoculation in the nasal and/or tracheal swabs of vaccinated macaques that were asymptomatic. On the other hand, the viruses were isolated from nasal and tracheal swabs from non-vaccinated macaques until day 5 and day 7 after inoculation of the H5N1 HPAI virus, respectively. Although six non-vaccinated macaques developed a high body temperature, and two of them lost their appetite after HPAI virus infection, they recovered by the end of the 12-day observation period and did not show the severe symptoms that have been reported in human H5N1 virus infection cases. This demonstrates that the vaccine prepared with the non-pathogenic H5N1 virus from our influenza virus library conferred protective immunity against H5N1 HPAI virus infection to macaques. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/18164788/A_vaccine_prepared_from_a_non_pathogenic_H5N1_avian_influenza_virus_strain_confers_protective_immunity_against_highly_pathogenic_avian_influenza_virus_infection_in_cynomolgus_macaques_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓21]

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