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A vaccine prepared from a non-pathogenic H5N1 avian influenza virus strain confers protective immunity against highly pathogenic avian influenza virus infection in cynomolgus macaques.
Vaccine. 2008 Jan 24; 26(4):562-72.V

Abstract

In order to prepare for the emergence of pandemic influenza viruses, we have established an influenza virus library that contains non-pathogenic influenza A virus strains with 135 combinations of 15 hemagglutinin and 9 neuraminidase subtypes. In this study, we developed a vaccine against H5N1 highly pathogenic avian influenza (HPAI) virus infection in humans using a virus strain selected from the library. We examined its immunogenic potency using cynomolgus macaques as a primate model. Virus antigen-specific antibodies were elicited by intranasal or subcutaneous administration of inactivated whole virus particle vaccines. After challenge with an H5N1 HPAI virus isolate obtained from a Vietnamese patient, the virus was detected only on next day following inoculation in the nasal and/or tracheal swabs of vaccinated macaques that were asymptomatic. On the other hand, the viruses were isolated from nasal and tracheal swabs from non-vaccinated macaques until day 5 and day 7 after inoculation of the H5N1 HPAI virus, respectively. Although six non-vaccinated macaques developed a high body temperature, and two of them lost their appetite after HPAI virus infection, they recovered by the end of the 12-day observation period and did not show the severe symptoms that have been reported in human H5N1 virus infection cases. This demonstrates that the vaccine prepared with the non-pathogenic H5N1 virus from our influenza virus library conferred protective immunity against H5N1 HPAI virus infection to macaques.

Authors+Show Affiliations

Department of Pathology, Shiga University of Medical Science, Otsu, Shiga, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18164788

Citation

Itoh, Yasushi, et al. "A Vaccine Prepared From a Non-pathogenic H5N1 Avian Influenza Virus Strain Confers Protective Immunity Against Highly Pathogenic Avian Influenza Virus Infection in Cynomolgus Macaques." Vaccine, vol. 26, no. 4, 2008, pp. 562-72.
Itoh Y, Ozaki H, Tsuchiya H, et al. A vaccine prepared from a non-pathogenic H5N1 avian influenza virus strain confers protective immunity against highly pathogenic avian influenza virus infection in cynomolgus macaques. Vaccine. 2008;26(4):562-72.
Itoh, Y., Ozaki, H., Tsuchiya, H., Okamoto, K., Torii, R., Sakoda, Y., Kawaoka, Y., Ogasawara, K., & Kida, H. (2008). A vaccine prepared from a non-pathogenic H5N1 avian influenza virus strain confers protective immunity against highly pathogenic avian influenza virus infection in cynomolgus macaques. Vaccine, 26(4), 562-72. https://doi.org/10.1016/j.vaccine.2007.11.031
Itoh Y, et al. A Vaccine Prepared From a Non-pathogenic H5N1 Avian Influenza Virus Strain Confers Protective Immunity Against Highly Pathogenic Avian Influenza Virus Infection in Cynomolgus Macaques. Vaccine. 2008 Jan 24;26(4):562-72. PubMed PMID: 18164788.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A vaccine prepared from a non-pathogenic H5N1 avian influenza virus strain confers protective immunity against highly pathogenic avian influenza virus infection in cynomolgus macaques. AU - Itoh,Yasushi, AU - Ozaki,Hiroichi, AU - Tsuchiya,Hideaki, AU - Okamoto,Kiyoko, AU - Torii,Ryuzo, AU - Sakoda,Yoshihiro, AU - Kawaoka,Yoshihiro, AU - Ogasawara,Kazumasa, AU - Kida,Hiroshi, Y1 - 2007/12/03/ PY - 2007/05/29/received PY - 2007/09/28/revised PY - 2007/11/04/accepted PY - 2008/1/1/pubmed PY - 2008/4/12/medline PY - 2008/1/1/entrez SP - 562 EP - 72 JF - Vaccine JO - Vaccine VL - 26 IS - 4 N2 - In order to prepare for the emergence of pandemic influenza viruses, we have established an influenza virus library that contains non-pathogenic influenza A virus strains with 135 combinations of 15 hemagglutinin and 9 neuraminidase subtypes. In this study, we developed a vaccine against H5N1 highly pathogenic avian influenza (HPAI) virus infection in humans using a virus strain selected from the library. We examined its immunogenic potency using cynomolgus macaques as a primate model. Virus antigen-specific antibodies were elicited by intranasal or subcutaneous administration of inactivated whole virus particle vaccines. After challenge with an H5N1 HPAI virus isolate obtained from a Vietnamese patient, the virus was detected only on next day following inoculation in the nasal and/or tracheal swabs of vaccinated macaques that were asymptomatic. On the other hand, the viruses were isolated from nasal and tracheal swabs from non-vaccinated macaques until day 5 and day 7 after inoculation of the H5N1 HPAI virus, respectively. Although six non-vaccinated macaques developed a high body temperature, and two of them lost their appetite after HPAI virus infection, they recovered by the end of the 12-day observation period and did not show the severe symptoms that have been reported in human H5N1 virus infection cases. This demonstrates that the vaccine prepared with the non-pathogenic H5N1 virus from our influenza virus library conferred protective immunity against H5N1 HPAI virus infection to macaques. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/18164788/A_vaccine_prepared_from_a_non_pathogenic_H5N1_avian_influenza_virus_strain_confers_protective_immunity_against_highly_pathogenic_avian_influenza_virus_infection_in_cynomolgus_macaques_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(07)01281-9 DB - PRIME DP - Unbound Medicine ER -