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Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
Vet Microbiol. 2008 Jun 22; 129(3-4):367-77.VM

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are pathogens, which can significantly affect the swine industry worldwide. Field surveys suggest that simultaneous PRRSV and PCV2 infection is common in pigs. The objective of this study was to measure the changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with PRRSV and PCV2, in order to analyze the synergistic influence of co-infection on the immune system. Changes in peripheral blood leukocyte subpopulations were systematically measured by flow cytometry (FCM). The levels of antibodies to PRRSV and PCV2 were detected by indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) and the indirect fluorescent antibody test (IFA), respectively. Serum viral loads were measured using real-time PCR. The results showed that piglets co-infected with PRRSV and PCV2 exhibited slower generation and lower levels of antibodies to PRRSV and PCV2, and increased amounts and a prolonged presence of both PRRSV and PCV2 in serum, in comparison to the piglets infected with either virus alone. The major finding in our study was that the total and differential leukocyte counts, including white blood cells (WBCs), monocytes, granulocytes and lymphocytes (T, B and NK cells, as well as T-cell subpopulations), dramatically decreased early during co-infection with PRRSV and PCV2 for about two weeks, in contrast with animals singly infected with either PRRSV or PCV2. These results suggest that PRRSV and PCV2 co-infection results in a synergistic decrease in immune cells in the peripheral blood of piglets. These data contribute to the understanding of the immunosuppressive effects resulting from PRRSV and PCV2 co-infection in pigs.

Authors+Show Affiliations

Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China Agricultural University, Haidian District, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18164875

Citation

Shi, Kaichuang, et al. "Changes in Peripheral Blood Leukocyte Subpopulations in Piglets Co-infected Experimentally With Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus Type 2." Veterinary Microbiology, vol. 129, no. 3-4, 2008, pp. 367-77.
Shi K, Li H, Guo X, et al. Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Vet Microbiol. 2008;129(3-4):367-77.
Shi, K., Li, H., Guo, X., Ge, X., Jia, H., Zheng, S., & Yang, H. (2008). Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Veterinary Microbiology, 129(3-4), 367-77. https://doi.org/10.1016/j.vetmic.2007.11.020
Shi K, et al. Changes in Peripheral Blood Leukocyte Subpopulations in Piglets Co-infected Experimentally With Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus Type 2. Vet Microbiol. 2008 Jun 22;129(3-4):367-77. PubMed PMID: 18164875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. AU - Shi,Kaichuang, AU - Li,Huanrong, AU - Guo,Xin, AU - Ge,Xinna, AU - Jia,Hong, AU - Zheng,Shijun, AU - Yang,Hanchun, Y1 - 2007/11/29/ PY - 2007/07/13/received PY - 2007/11/21/revised PY - 2007/11/22/accepted PY - 2008/1/1/pubmed PY - 2008/11/5/medline PY - 2008/1/1/entrez SP - 367 EP - 77 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 129 IS - 3-4 N2 - Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are pathogens, which can significantly affect the swine industry worldwide. Field surveys suggest that simultaneous PRRSV and PCV2 infection is common in pigs. The objective of this study was to measure the changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with PRRSV and PCV2, in order to analyze the synergistic influence of co-infection on the immune system. Changes in peripheral blood leukocyte subpopulations were systematically measured by flow cytometry (FCM). The levels of antibodies to PRRSV and PCV2 were detected by indirect Enzyme-Linked ImmunoSorbent Assay (ELISA) and the indirect fluorescent antibody test (IFA), respectively. Serum viral loads were measured using real-time PCR. The results showed that piglets co-infected with PRRSV and PCV2 exhibited slower generation and lower levels of antibodies to PRRSV and PCV2, and increased amounts and a prolonged presence of both PRRSV and PCV2 in serum, in comparison to the piglets infected with either virus alone. The major finding in our study was that the total and differential leukocyte counts, including white blood cells (WBCs), monocytes, granulocytes and lymphocytes (T, B and NK cells, as well as T-cell subpopulations), dramatically decreased early during co-infection with PRRSV and PCV2 for about two weeks, in contrast with animals singly infected with either PRRSV or PCV2. These results suggest that PRRSV and PCV2 co-infection results in a synergistic decrease in immune cells in the peripheral blood of piglets. These data contribute to the understanding of the immunosuppressive effects resulting from PRRSV and PCV2 co-infection in pigs. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/18164875/Changes_in_peripheral_blood_leukocyte_subpopulations_in_piglets_co_infected_experimentally_with_porcine_reproductive_and_respiratory_syndrome_virus_and_porcine_circovirus_type_2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(07)00578-0 DB - PRIME DP - Unbound Medicine ER -