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In vitro efficacy of an ophthalmic drug combination against corneal pathogens of horses.
Am J Vet Res. 2008 Jan; 69(1):101-7.AJ

Abstract

OBJECTIVE

To evaluate the in vitro efficacy of an ophthalmic drug combination against common corneal pathogens of horses.

SAMPLE POPULATION

Representative isolates of 3 bacterial and 2 fungal corneal pathogens of horses.

PROCEDURES

Pathogens were subjected to minimum inhibitory concentration (MIC) testing of a drug combination that consisted of equal volumes of natamycin 3.33%, tobramycin 0.3%, cefazolin 5.5%, and equine serum. Proteinase inhibitory activity of the drug combination was assessed by use of a fluorescence microplate assay with gelatin and collagen I as substrates. The MICs of the drug combination were compared with those for each of the component medications and antiproteinase activity of the drug combination was compared with that of serum by use of paired t tests and a 2-way ANOVA, respectively.

RESULTS

The drug combination was at least as effective as each medication separately for inhibiting microbial growth of all pathogens tested and was significantly more effective against B-hemolytic Streptococcus spp, Aspergillus spp, and Fusarium spp than the relevant medications separately. Serum and the drug combination both had significant antigelatinase activity, and serum had significant anticollagenase activity. Antiproteinase activity of serum was a concentration-dependent event, which enabled serum to achieve significantly greater activity than the drug combination after 3.5 and 4 hours of incubation [corrected] for the gelatin and collagen I assays, respectively.

CONCLUSIONS AND CLINICAL RELEVANCE

Drug combinations have the attractive potential of minimizing the time, stress, and fatigue associated with topical treatment regimens consisting of multiple drugs used separately for horses with keratitis.

Authors+Show Affiliations

University of Florida Veterinary Medical Center, Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18167094

Citation

Scotty, Nicole C., et al. "In Vitro Efficacy of an Ophthalmic Drug Combination Against Corneal Pathogens of Horses." American Journal of Veterinary Research, vol. 69, no. 1, 2008, pp. 101-7.
Scotty NC, Brooks DE, Schuman Rose CD. In vitro efficacy of an ophthalmic drug combination against corneal pathogens of horses. Am J Vet Res. 2008;69(1):101-7.
Scotty, N. C., Brooks, D. E., & Schuman Rose, C. D. (2008). In vitro efficacy of an ophthalmic drug combination against corneal pathogens of horses. American Journal of Veterinary Research, 69(1), 101-7. https://doi.org/10.2460/ajvr.69.1.101
Scotty NC, Brooks DE, Schuman Rose CD. In Vitro Efficacy of an Ophthalmic Drug Combination Against Corneal Pathogens of Horses. Am J Vet Res. 2008;69(1):101-7. PubMed PMID: 18167094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro efficacy of an ophthalmic drug combination against corneal pathogens of horses. AU - Scotty,Nicole C, AU - Brooks,Dennis E, AU - Schuman Rose,Crystal D, PY - 2008/1/3/pubmed PY - 2008/2/28/medline PY - 2008/1/3/entrez SP - 101 EP - 7 JF - American journal of veterinary research JO - Am. J. Vet. Res. VL - 69 IS - 1 N2 - OBJECTIVE: To evaluate the in vitro efficacy of an ophthalmic drug combination against common corneal pathogens of horses. SAMPLE POPULATION: Representative isolates of 3 bacterial and 2 fungal corneal pathogens of horses. PROCEDURES: Pathogens were subjected to minimum inhibitory concentration (MIC) testing of a drug combination that consisted of equal volumes of natamycin 3.33%, tobramycin 0.3%, cefazolin 5.5%, and equine serum. Proteinase inhibitory activity of the drug combination was assessed by use of a fluorescence microplate assay with gelatin and collagen I as substrates. The MICs of the drug combination were compared with those for each of the component medications and antiproteinase activity of the drug combination was compared with that of serum by use of paired t tests and a 2-way ANOVA, respectively. RESULTS: The drug combination was at least as effective as each medication separately for inhibiting microbial growth of all pathogens tested and was significantly more effective against B-hemolytic Streptococcus spp, Aspergillus spp, and Fusarium spp than the relevant medications separately. Serum and the drug combination both had significant antigelatinase activity, and serum had significant anticollagenase activity. Antiproteinase activity of serum was a concentration-dependent event, which enabled serum to achieve significantly greater activity than the drug combination after 3.5 and 4 hours of incubation [corrected] for the gelatin and collagen I assays, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Drug combinations have the attractive potential of minimizing the time, stress, and fatigue associated with topical treatment regimens consisting of multiple drugs used separately for horses with keratitis. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/18167094/In_vitro_efficacy_of_an_ophthalmic_drug_combination_against_corneal_pathogens_of_horses_ L2 - http://avmajournals.avma.org/doi/full/10.2460/ajvr.69.1.101?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -