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[Inhibition of NF-kappa B activity enhanced apoptosis induced by matrine in hepatocellular carcinoma cells].
Zhonghua Gan Zang Bing Za Zhi. 2007 Dec; 15(12):914-7.ZG

Abstract

OBJECTIVE

To investigate the relationship between activation of nuclear factor-kappa gene binding (NF-kappaB) and apoptosis induced by matrine in hepatocellular carcinoma cell line HepG2.

METHODS

HepG2 cells were stimulated by different concentrations of matrine (0.8, 1.0, 1.5, 2.0, 2.5 g/L). The HepG2 cell survival rates were evaluated by MTT assay. Cultured HepG2 cells were implanted in culture flasks and divided into four groups: a control group, a pyrrolidine dithiocarbamate (PDTC) group (20 micromol/L), a matrine group (1.5 g/L) and a combination group, PDTC (20 micromol/L) + matrine (1.5 g/L) combination group. Apoptosis induced by matrine was analyzed by flow cytometry (FCM) and TUNEL. The DNA-binding activity of NF-kappaB was determined by electrophoretic mobility shift assay (EMSA).

RESULTS

PDTC enhanced the inhibition of matrine on cell proliferation (F=183.92, P less than 0.01). The apoptosis and activation of NF-kappaB of HepG2 cells were induced by matrine. PDTC significantly suppressed NF-kappaB activation induced by matrine in HepG2 cells. PDTC increased the apoptosis induced by matrine of the HepG2 cells from 6.11% +/- 0.81% to 12.95% +/- 0.02%, chi2=9.67, P less than 0.01.

CONCLUSIONS

Matrine could induce apoptosis, and at the same time induce activation of NF-kappaB in HepG2 cells. PDTC increases the apoptosis in hepatocellular carcinoma cells and it may be related to suppressing NF-kB activation of HepG2 cells.

Authors+Show Affiliations

Department of Gastroenterology, Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18171526

Citation

Gao, Hang, et al. "[Inhibition of NF-kappa B Activity Enhanced Apoptosis Induced By Matrine in Hepatocellular Carcinoma Cells]." Zhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal of Hepatology, vol. 15, no. 12, 2007, pp. 914-7.
Gao H, He S, Tang WX, et al. [Inhibition of NF-kappa B activity enhanced apoptosis induced by matrine in hepatocellular carcinoma cells]. Zhonghua Gan Zang Bing Za Zhi. 2007;15(12):914-7.
Gao, H., He, S., Tang, W. X., & Wang, J. (2007). [Inhibition of NF-kappa B activity enhanced apoptosis induced by matrine in hepatocellular carcinoma cells]. Zhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal of Hepatology, 15(12), 914-7.
Gao H, et al. [Inhibition of NF-kappa B Activity Enhanced Apoptosis Induced By Matrine in Hepatocellular Carcinoma Cells]. Zhonghua Gan Zang Bing Za Zhi. 2007;15(12):914-7. PubMed PMID: 18171526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Inhibition of NF-kappa B activity enhanced apoptosis induced by matrine in hepatocellular carcinoma cells]. AU - Gao,Hang, AU - He,Song, AU - Tang,Wei-Xue, AU - Wang,Jue, PY - 2008/1/4/pubmed PY - 2010/7/7/medline PY - 2008/1/4/entrez SP - 914 EP - 7 JF - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology JO - Zhonghua Gan Zang Bing Za Zhi VL - 15 IS - 12 N2 - OBJECTIVE: To investigate the relationship between activation of nuclear factor-kappa gene binding (NF-kappaB) and apoptosis induced by matrine in hepatocellular carcinoma cell line HepG2. METHODS: HepG2 cells were stimulated by different concentrations of matrine (0.8, 1.0, 1.5, 2.0, 2.5 g/L). The HepG2 cell survival rates were evaluated by MTT assay. Cultured HepG2 cells were implanted in culture flasks and divided into four groups: a control group, a pyrrolidine dithiocarbamate (PDTC) group (20 micromol/L), a matrine group (1.5 g/L) and a combination group, PDTC (20 micromol/L) + matrine (1.5 g/L) combination group. Apoptosis induced by matrine was analyzed by flow cytometry (FCM) and TUNEL. The DNA-binding activity of NF-kappaB was determined by electrophoretic mobility shift assay (EMSA). RESULTS: PDTC enhanced the inhibition of matrine on cell proliferation (F=183.92, P less than 0.01). The apoptosis and activation of NF-kappaB of HepG2 cells were induced by matrine. PDTC significantly suppressed NF-kappaB activation induced by matrine in HepG2 cells. PDTC increased the apoptosis induced by matrine of the HepG2 cells from 6.11% +/- 0.81% to 12.95% +/- 0.02%, chi2=9.67, P less than 0.01. CONCLUSIONS: Matrine could induce apoptosis, and at the same time induce activation of NF-kappaB in HepG2 cells. PDTC increases the apoptosis in hepatocellular carcinoma cells and it may be related to suppressing NF-kB activation of HepG2 cells. SN - 1007-3418 UR - https://www.unboundmedicine.com/medline/citation/18171526/[Inhibition_of_NF_kappa_B_activity_enhanced_apoptosis_induced_by_matrine_in_hepatocellular_carcinoma_cells]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=1007-3418&year=2007&vol=15&issue=12&fpage=914 DB - PRIME DP - Unbound Medicine ER -