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Characterization of CD4+ FOXP3+ T-cell clones established from chronic inflammatory lesions.
Oral Microbiol Immunol. 2008 Feb; 23(1):49-54.OM

Abstract

INTRODUCTION

Our previous study demonstrated that the gene expression of FOXP3, a characteristic marker for CD4(+) CD25(+) regulatory T cells in mice, is upregulated more in periodontitis than in gingivitis at the messenger RNA (mRNA) level. Furthermore, most of the T-cell clones established from periodontitis lesions expressed FOXP3 mRNA. However, role of the FOXP3(+) gingival T cells has not been elucidated.

METHODS

The phenotype of FOXP3-expressing cells in periodontitis lesions was determined immunohistochemically. CD4(+) FOXP3(+) gingival T-cell clones were established from three patients with advanced periodontitis by using immunomagnetic beads. Gene expression and phenotype analyses were performed by reverse-transcription polymerase chain reactions and flow cytometry, respectively. The effect of CD4(+) FOXP3(+) T-cell clones on the proliferative response of CD4(+) CD25(-) T cells was examined by [(3)H]thymidine incorporation.

RESULTS

FOXP3 expression was found in some CD4(+) T cells and CD25(+) cells but not in CD8(+) T cells by immunohistochemistry. In spite of a substantial expression of the CD25 gene, the expression level of membrane CD25 on the CD4(+) FOXP3(+) gingival T-cell clones was low. While peripheral blood CD4(+) CD25(+) FOXP3(+) cells suppressed the proliferation of CD4(+) CD25(-) T cells, the CD4(+) CD25(low) FOXP3(+) gingival T-cell clones enhanced the proliferation significantly.

CONCLUSION

Our study makes it evident that most, if not all, of the FOXP3(+) T cells in periodontitis lesions can be considered to be effector T cells. The effector activity of the gingival T-cell clones could be attributable to the low level of membrane CD25 expression. Further studies are clearly needed to clarify the role of these T cells and their unique characteristics in the pathogenesis of periodontal disease.

Authors+Show Affiliations

Laboratory of Periodontology and Immunology, Department of Oral Health and Welfare, Niigata University Faculty of Dentistry, Niigata, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18173798

Citation

Okui, T, et al. "Characterization of CD4+ FOXP3+ T-cell Clones Established From Chronic Inflammatory Lesions." Oral Microbiology and Immunology, vol. 23, no. 1, 2008, pp. 49-54.
Okui T, Ito H, Honda T, et al. Characterization of CD4+ FOXP3+ T-cell clones established from chronic inflammatory lesions. Oral Microbiol Immunol. 2008;23(1):49-54.
Okui, T., Ito, H., Honda, T., Amanuma, R., Yoshie, H., & Yamazaki, K. (2008). Characterization of CD4+ FOXP3+ T-cell clones established from chronic inflammatory lesions. Oral Microbiology and Immunology, 23(1), 49-54. https://doi.org/10.1111/j.1399-302X.2007.00390.x
Okui T, et al. Characterization of CD4+ FOXP3+ T-cell Clones Established From Chronic Inflammatory Lesions. Oral Microbiol Immunol. 2008;23(1):49-54. PubMed PMID: 18173798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of CD4+ FOXP3+ T-cell clones established from chronic inflammatory lesions. AU - Okui,T, AU - Ito,H, AU - Honda,T, AU - Amanuma,R, AU - Yoshie,H, AU - Yamazaki,K, PY - 2008/1/5/pubmed PY - 2008/4/26/medline PY - 2008/1/5/entrez SP - 49 EP - 54 JF - Oral microbiology and immunology JO - Oral Microbiol. Immunol. VL - 23 IS - 1 N2 - INTRODUCTION: Our previous study demonstrated that the gene expression of FOXP3, a characteristic marker for CD4(+) CD25(+) regulatory T cells in mice, is upregulated more in periodontitis than in gingivitis at the messenger RNA (mRNA) level. Furthermore, most of the T-cell clones established from periodontitis lesions expressed FOXP3 mRNA. However, role of the FOXP3(+) gingival T cells has not been elucidated. METHODS: The phenotype of FOXP3-expressing cells in periodontitis lesions was determined immunohistochemically. CD4(+) FOXP3(+) gingival T-cell clones were established from three patients with advanced periodontitis by using immunomagnetic beads. Gene expression and phenotype analyses were performed by reverse-transcription polymerase chain reactions and flow cytometry, respectively. The effect of CD4(+) FOXP3(+) T-cell clones on the proliferative response of CD4(+) CD25(-) T cells was examined by [(3)H]thymidine incorporation. RESULTS: FOXP3 expression was found in some CD4(+) T cells and CD25(+) cells but not in CD8(+) T cells by immunohistochemistry. In spite of a substantial expression of the CD25 gene, the expression level of membrane CD25 on the CD4(+) FOXP3(+) gingival T-cell clones was low. While peripheral blood CD4(+) CD25(+) FOXP3(+) cells suppressed the proliferation of CD4(+) CD25(-) T cells, the CD4(+) CD25(low) FOXP3(+) gingival T-cell clones enhanced the proliferation significantly. CONCLUSION: Our study makes it evident that most, if not all, of the FOXP3(+) T cells in periodontitis lesions can be considered to be effector T cells. The effector activity of the gingival T-cell clones could be attributable to the low level of membrane CD25 expression. Further studies are clearly needed to clarify the role of these T cells and their unique characteristics in the pathogenesis of periodontal disease. SN - 0902-0055 UR - https://www.unboundmedicine.com/medline/citation/18173798/Characterization_of_CD4+_FOXP3+_T_cell_clones_established_from_chronic_inflammatory_lesions_ L2 - https://doi.org/10.1111/j.1399-302X.2007.00390.x DB - PRIME DP - Unbound Medicine ER -