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Progesterone and interferon tau regulate hypoxia-inducible factors in the endometrium of the ovine uterus.
Endocrinology. 2008 Apr; 149(4):1926-34.E

Abstract

In ruminants, progesterone (P4) from the ovary and interferon tau (IFNT) from the elongating blastocyst regulate expression of genes in the endometrium that are hypothesized to be important for uterine receptivity and blastocyst development. These studies determined effects of the estrous cycle, pregnancy, P4, and IFNT on hypoxia-inducible factor (HIF) expression in the ovine uterus. HIF1A mRNA, HIF2A mRNA, and HIF2A protein were most abundant in the endometrial luminal and superficial glandular epithelia (LE and sGE, respectively) of the uterus and conceptus trophectoderm. During the estrous cycle, HIF1A and HIF2A mRNA levels were low to undetectable on d 10 in the endometrial LE/sGE, increased between d 10 and 14, and then declined on d 16. Both HIF1A and HIF2A mRNA were more abundant in the endometrial LE/sGE of pregnant ewes. However, HIF3A, HIF1B, HIF2B, and HIF3B mRNA abundance was low in most cell types of the endometria and conceptus. Treatment of ovariectomized ewes with P4 induced HIF1A and HIF2A in the endometrial LE/sGE, and intrauterine infusion of ovine IFNT further increased HIF2A in P4-treated ewes, but not in ewes treated with P4 and the antiprogestin ZK 136,317. HIF3A, HIF1B, HIF2B, and HIF3B mRNA abundance was not regulated by either P4 or IFNT. Two HIF-responsive genes, carboxy-terminal domain 2 and vascular endothelial growth factor A, were detected in both the endometrium and conceptus. These studies identified new P4-induced (HIF1A and HIF2A) and IFNT-stimulated (HIF2A) genes in the uterine LE/sGE, and implicate the HIF pathway in regulation of endometrial epithelial functions and angiogenesis, as well as peri-implantation blastocyst development.

Authors+Show Affiliations

Center for Animal Biotechnology and Genomics, Texas A&M University, College Station, TX 77843-2471, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

18174278

Citation

Song, Gwonhwa, et al. "Progesterone and Interferon Tau Regulate Hypoxia-inducible Factors in the Endometrium of the Ovine Uterus." Endocrinology, vol. 149, no. 4, 2008, pp. 1926-34.
Song G, Kim J, Bazer FW, et al. Progesterone and interferon tau regulate hypoxia-inducible factors in the endometrium of the ovine uterus. Endocrinology. 2008;149(4):1926-34.
Song, G., Kim, J., Bazer, F. W., & Spencer, T. E. (2008). Progesterone and interferon tau regulate hypoxia-inducible factors in the endometrium of the ovine uterus. Endocrinology, 149(4), 1926-34. https://doi.org/10.1210/en.2007-1530
Song G, et al. Progesterone and Interferon Tau Regulate Hypoxia-inducible Factors in the Endometrium of the Ovine Uterus. Endocrinology. 2008;149(4):1926-34. PubMed PMID: 18174278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Progesterone and interferon tau regulate hypoxia-inducible factors in the endometrium of the ovine uterus. AU - Song,Gwonhwa, AU - Kim,Jinyoung, AU - Bazer,Fuller W, AU - Spencer,Thomas E, Y1 - 2008/01/03/ PY - 2008/1/5/pubmed PY - 2008/5/7/medline PY - 2008/1/5/entrez SP - 1926 EP - 34 JF - Endocrinology JO - Endocrinology VL - 149 IS - 4 N2 - In ruminants, progesterone (P4) from the ovary and interferon tau (IFNT) from the elongating blastocyst regulate expression of genes in the endometrium that are hypothesized to be important for uterine receptivity and blastocyst development. These studies determined effects of the estrous cycle, pregnancy, P4, and IFNT on hypoxia-inducible factor (HIF) expression in the ovine uterus. HIF1A mRNA, HIF2A mRNA, and HIF2A protein were most abundant in the endometrial luminal and superficial glandular epithelia (LE and sGE, respectively) of the uterus and conceptus trophectoderm. During the estrous cycle, HIF1A and HIF2A mRNA levels were low to undetectable on d 10 in the endometrial LE/sGE, increased between d 10 and 14, and then declined on d 16. Both HIF1A and HIF2A mRNA were more abundant in the endometrial LE/sGE of pregnant ewes. However, HIF3A, HIF1B, HIF2B, and HIF3B mRNA abundance was low in most cell types of the endometria and conceptus. Treatment of ovariectomized ewes with P4 induced HIF1A and HIF2A in the endometrial LE/sGE, and intrauterine infusion of ovine IFNT further increased HIF2A in P4-treated ewes, but not in ewes treated with P4 and the antiprogestin ZK 136,317. HIF3A, HIF1B, HIF2B, and HIF3B mRNA abundance was not regulated by either P4 or IFNT. Two HIF-responsive genes, carboxy-terminal domain 2 and vascular endothelial growth factor A, were detected in both the endometrium and conceptus. These studies identified new P4-induced (HIF1A and HIF2A) and IFNT-stimulated (HIF2A) genes in the uterine LE/sGE, and implicate the HIF pathway in regulation of endometrial epithelial functions and angiogenesis, as well as peri-implantation blastocyst development. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/18174278/Progesterone_and_interferon_tau_regulate_hypoxia_inducible_factors_in_the_endometrium_of_the_ovine_uterus_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2007-1530 DB - PRIME DP - Unbound Medicine ER -