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A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum.
Glycobiology. 2008 Mar; 18(3):250-9.G

Abstract

The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, approximately 14 kDa, and SG 2, approximately 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H 1D NMR spectra. The structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. In minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1'carboxy)-ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Farias EH
Laboratório de Biotecnologia de Polímeros Naturais, Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte, Natal, RN, 59072-970, Brazil.
Pomin VH
No affiliation info available
Valente AP
No affiliation info available
Nader HB
No affiliation info available
Rocha HA
No affiliation info available
Mourão PA
No affiliation info available

MeSH

Carbohydrate ConformationChlorophytaElectrophoresis, Agar GelElectrophoresis, Polyacrylamide GelGalactansMagnetic Resonance SpectroscopyReference StandardsSpecies SpecificitySulfates

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18174311

Citation

Farias, Eduardo H C., et al. "A Preponderantly 4-sulfated, 3-linked Galactan From the Green Alga Codium Isthmocladum." Glycobiology, vol. 18, no. 3, 2008, pp. 250-9.
Farias EH, Pomin VH, Valente AP, et al. A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum. Glycobiology. 2008;18(3):250-9.
Farias, E. H., Pomin, V. H., Valente, A. P., Nader, H. B., Rocha, H. A., & Mourão, P. A. (2008). A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum. Glycobiology, 18(3), 250-9. https://doi.org/10.1093/glycob/cwm139
Farias EH, et al. A Preponderantly 4-sulfated, 3-linked Galactan From the Green Alga Codium Isthmocladum. Glycobiology. 2008;18(3):250-9. PubMed PMID: 18174311.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A preponderantly 4-sulfated, 3-linked galactan from the green alga Codium isthmocladum. AU - Farias,Eduardo H C, AU - Pomin,Vitor H, AU - Valente,Ana-Paula, AU - Nader,Helena B, AU - Rocha,Hugo A O, AU - Mourão,Paulo A S, Y1 - 2008/01/03/ PY - 2008/1/5/pubmed PY - 2008/7/30/medline PY - 2008/1/5/entrez SP - 250 EP - 9 JF - Glycobiology JO - Glycobiology VL - 18 IS - 3 N2 - The green algae of the genus Codium have recently been demonstrated to be an important source of sulfated galactans from the marine environment. Here, a sulfated galactan was isolated from the species Codium isthmocladum and its structure was studied by a combination of chemical analyses and NMR spectroscopy. Two fractions (SG 1, approximately 14 kDa, and SG 2, approximately 20 kDa) were derived from this highly polydisperse and heterogeneous polysaccharide. Both exhibited similar structures in (1)H 1D NMR spectra. The structural features of SG 2 and its desulfated derivative were analyzed by COSY, TOCSY, DEPT-HSQC, HSQC, and HMBC. This sulfated galactan is composed preponderantly of 4-sulfated, 3-linked beta-D-galactopyranosyl units. In minor amounts, it is sulfated and glycosylated at C-6. Pyruvate groups are also found, forming five-membered cyclic ketals as 3,4-O-(1'carboxy)-ethylidene-beta-D-galactose residues. A comparison of sulfated galactans from different marine taxonomic groups revealed similar backbones of 3-beta-D-Galp-1. SN - 1460-2423 UR - https://www.unboundmedicine.com/medline/citation/18174311/A_preponderantly_4_sulfated_3_linked_galactan_from_the_green_alga_Codium_isthmocladum_ DB - PRIME DP - Unbound Medicine ER -