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Mechanism of ischemic tolerance induced by hyperbaric oxygen preconditioning involves upregulation of hypoxia-inducible factor-1alpha and erythropoietin in rats.
J Appl Physiol (1985). 2008 Apr; 104(4):1185-91.JA

Abstract

We studied the effect of hyperbaric oxygen (HBO) preconditioning on the molecular mechanisms of neuroprotection in a rat focal cerebral ischemic model. Seventy-two male Sprague-Dawley rats were pretreated with HBO (100% O(2), 2 atmospheres absolute, 1 h once every other day for 5 sessions) or with room air. In experiment 1, HBO-preconditioned rats and matched room air controls were subjected to focal cerebral ischemia or sham surgery. Postinjury motor parameters and infarction volumes of HBO-preconditioned rats were compared with those of controls. In experiment 2, HBO-preconditioned rats and matched room air controls were killed at different time points. Brain levels of hypoxia-inducible factor-1alpha (HIF-1alpha) and its downstream target gene erythropoietin (EPO) analyzed by Western blotting and RT-PCR as well as HIF-1alpha DNA-binding and transcriptional activities were determined in the ipsilateral hemisphere. HBO induced a marked increase in the protein expressions of HIF-1alpha and EPO and the activity of HIF-1alpha, as well as the expression of EPO mRNA. HBO preconditioning dramatically improved the neurobehavioral outcome at all time points (3.0 +/- 2.1 vs. 5.6 +/- 1.5 at 4 h, 5.0 +/- 1.8 vs. 8.8 +/- 1.4 at 8 h, 6.4 +/- 1.8 vs. 9.7 +/- 1.3 at 24 h; P < 0.01, respectively) and reduced infarction volumes (20.7 +/- 4.5 vs. 12.5 +/- 3.6%, 2,3,5-Triphenyltetrazolium chloride staining) after cerebral ischemia. This observation indicates that the neuroprotection induced by HBO preconditioning may be mediated by an upregulation of HIF-1alpha and its target gene EPO.

Authors+Show Affiliations

Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18174394

Citation

Gu, Guo-Jun, et al. "Mechanism of Ischemic Tolerance Induced By Hyperbaric Oxygen Preconditioning Involves Upregulation of Hypoxia-inducible Factor-1alpha and Erythropoietin in Rats." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 104, no. 4, 2008, pp. 1185-91.
Gu GJ, Li YP, Peng ZY, et al. Mechanism of ischemic tolerance induced by hyperbaric oxygen preconditioning involves upregulation of hypoxia-inducible factor-1alpha and erythropoietin in rats. J Appl Physiol. 2008;104(4):1185-91.
Gu, G. J., Li, Y. P., Peng, Z. Y., Xu, J. J., Kang, Z. M., Xu, W. G., Tao, H. Y., Ostrowski, R. P., Zhang, J. H., & Sun, X. J. (2008). Mechanism of ischemic tolerance induced by hyperbaric oxygen preconditioning involves upregulation of hypoxia-inducible factor-1alpha and erythropoietin in rats. Journal of Applied Physiology (Bethesda, Md. : 1985), 104(4), 1185-91. https://doi.org/10.1152/japplphysiol.00323.2007
Gu GJ, et al. Mechanism of Ischemic Tolerance Induced By Hyperbaric Oxygen Preconditioning Involves Upregulation of Hypoxia-inducible Factor-1alpha and Erythropoietin in Rats. J Appl Physiol. 2008;104(4):1185-91. PubMed PMID: 18174394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mechanism of ischemic tolerance induced by hyperbaric oxygen preconditioning involves upregulation of hypoxia-inducible factor-1alpha and erythropoietin in rats. AU - Gu,Guo-Jun, AU - Li,Yun-Ping, AU - Peng,Zao-Yun, AU - Xu,Jia-Jun, AU - Kang,Zhi-Min, AU - Xu,Wei-Gang, AU - Tao,Heng-Yi, AU - Ostrowski,Robert P, AU - Zhang,John H, AU - Sun,Xue-Jun, Y1 - 2008/01/03/ PY - 2008/1/5/pubmed PY - 2008/6/18/medline PY - 2008/1/5/entrez SP - 1185 EP - 91 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J. Appl. Physiol. VL - 104 IS - 4 N2 - We studied the effect of hyperbaric oxygen (HBO) preconditioning on the molecular mechanisms of neuroprotection in a rat focal cerebral ischemic model. Seventy-two male Sprague-Dawley rats were pretreated with HBO (100% O(2), 2 atmospheres absolute, 1 h once every other day for 5 sessions) or with room air. In experiment 1, HBO-preconditioned rats and matched room air controls were subjected to focal cerebral ischemia or sham surgery. Postinjury motor parameters and infarction volumes of HBO-preconditioned rats were compared with those of controls. In experiment 2, HBO-preconditioned rats and matched room air controls were killed at different time points. Brain levels of hypoxia-inducible factor-1alpha (HIF-1alpha) and its downstream target gene erythropoietin (EPO) analyzed by Western blotting and RT-PCR as well as HIF-1alpha DNA-binding and transcriptional activities were determined in the ipsilateral hemisphere. HBO induced a marked increase in the protein expressions of HIF-1alpha and EPO and the activity of HIF-1alpha, as well as the expression of EPO mRNA. HBO preconditioning dramatically improved the neurobehavioral outcome at all time points (3.0 +/- 2.1 vs. 5.6 +/- 1.5 at 4 h, 5.0 +/- 1.8 vs. 8.8 +/- 1.4 at 8 h, 6.4 +/- 1.8 vs. 9.7 +/- 1.3 at 24 h; P < 0.01, respectively) and reduced infarction volumes (20.7 +/- 4.5 vs. 12.5 +/- 3.6%, 2,3,5-Triphenyltetrazolium chloride staining) after cerebral ischemia. This observation indicates that the neuroprotection induced by HBO preconditioning may be mediated by an upregulation of HIF-1alpha and its target gene EPO. SN - 8750-7587 UR - https://www.unboundmedicine.com/medline/citation/18174394/Mechanism_of_ischemic_tolerance_induced_by_hyperbaric_oxygen_preconditioning_involves_upregulation_of_hypoxia_inducible_factor_1alpha_and_erythropoietin_in_rats_ L2 - http://www.physiology.org/doi/full/10.1152/japplphysiol.00323.2007?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -