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A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300.
J Infect Dis. 2008 Jan 15; 197(2):187-94.JI

Abstract

BACKGROUND

The majority of recent community-associated methicillin-resistant Staphylococcus aureus (MRSA) infections in the United States have been caused by a single clone, USA300. USA300 secretes Panton-Valentine leukocidin (PVL) toxin, which is associated with highly virulent infections.

METHODS

We sequenced the PVL genes of 174 S. aureus isolates from a global clinical sample. We combined phylogenetic reconstruction and protein modeling methods to analyze genetic variation in PVL.

RESULTS

Nucleotide variation was detected at 12 of 1726 sites. Two PVL sequence variants, the R variant and the H variant, were identified on the basis of a substitution at nt 527. Of sequences obtained in the United States, 96.7% harbor the R variant, whereas 95.6% of sequences obtained outside the United States harbor the H variant; 91.3% of MRSA isolates harbor the R variant, and 91.3% of methicillin-susceptible strains harbor the H variant. A molecular model of PVL shows 3 mechanisms by which the amino acid substitution may affect PVL function.

CONCLUSIONS

All sampled PVL genes appear to share a recent common ancestor and spread via a combination of clonal expansion and horizontal transfer. US isolates harbor a variant of PVL that is strongly associated with MRSA infections. Protein modeling reveals that this variant may have functional significance. We propose a hypothesis for the origin of USA300.

Authors+Show Affiliations

Evolutionary and Structural Bioinformatics, Collegeville, Pennsylvania, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18177252

Citation

O'Hara, F Patrick, et al. "A Geographic Variant of the Staphylococcus Aureus Panton-Valentine Leukocidin Toxin and the Origin of Community-associated Methicillin-resistant S. Aureus USA300." The Journal of Infectious Diseases, vol. 197, no. 2, 2008, pp. 187-94.
O'Hara FP, Guex N, Word JM, et al. A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300. J Infect Dis. 2008;197(2):187-94.
O'Hara, F. P., Guex, N., Word, J. M., Miller, L. A., Becker, J. A., Walsh, S. L., Scangarella, N. E., West, J. M., Shawar, R. M., & Amrine-Madsen, H. (2008). A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300. The Journal of Infectious Diseases, 197(2), 187-94. https://doi.org/10.1086/524684
O'Hara FP, et al. A Geographic Variant of the Staphylococcus Aureus Panton-Valentine Leukocidin Toxin and the Origin of Community-associated Methicillin-resistant S. Aureus USA300. J Infect Dis. 2008 Jan 15;197(2):187-94. PubMed PMID: 18177252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A geographic variant of the Staphylococcus aureus Panton-Valentine leukocidin toxin and the origin of community-associated methicillin-resistant S. aureus USA300. AU - O'Hara,F Patrick, AU - Guex,Nicolas, AU - Word,J Michael, AU - Miller,Linda A, AU - Becker,Julie A, AU - Walsh,Stacey L, AU - Scangarella,Nicole E, AU - West,Joshua M, AU - Shawar,Ribhi M, AU - Amrine-Madsen,Heather, PY - 2008/1/8/pubmed PY - 2008/2/20/medline PY - 2008/1/8/entrez SP - 187 EP - 94 JF - The Journal of infectious diseases JO - J Infect Dis VL - 197 IS - 2 N2 - BACKGROUND: The majority of recent community-associated methicillin-resistant Staphylococcus aureus (MRSA) infections in the United States have been caused by a single clone, USA300. USA300 secretes Panton-Valentine leukocidin (PVL) toxin, which is associated with highly virulent infections. METHODS: We sequenced the PVL genes of 174 S. aureus isolates from a global clinical sample. We combined phylogenetic reconstruction and protein modeling methods to analyze genetic variation in PVL. RESULTS: Nucleotide variation was detected at 12 of 1726 sites. Two PVL sequence variants, the R variant and the H variant, were identified on the basis of a substitution at nt 527. Of sequences obtained in the United States, 96.7% harbor the R variant, whereas 95.6% of sequences obtained outside the United States harbor the H variant; 91.3% of MRSA isolates harbor the R variant, and 91.3% of methicillin-susceptible strains harbor the H variant. A molecular model of PVL shows 3 mechanisms by which the amino acid substitution may affect PVL function. CONCLUSIONS: All sampled PVL genes appear to share a recent common ancestor and spread via a combination of clonal expansion and horizontal transfer. US isolates harbor a variant of PVL that is strongly associated with MRSA infections. Protein modeling reveals that this variant may have functional significance. We propose a hypothesis for the origin of USA300. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/18177252/A_geographic_variant_of_the_Staphylococcus_aureus_Panton_Valentine_leukocidin_toxin_and_the_origin_of_community_associated_methicillin_resistant_S__aureus_USA300_ DB - PRIME DP - Unbound Medicine ER -