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Synthesis, characterization and stability of a luteinizing hormone-releasing hormone (LHRH)-functionalized poly(amidoamine) dendrimer conjugate.

Abstract

Cancer targeting is crucial for cancer detection, therapy and targeted drug delivery. A dendrimer-peptide conjugate has been synthesized based on poly(amidoamine) dendrimer generation 5 (PAMAM G5) as a platform and a luteinizing hormone-releasing hormone (LHRH) peptide as a targeting moiety. The synthesized conjugate was fully characterized using nuclear magnetic resonance (NMR), UV-Vis spectrometry, reverse-phase high-performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Further stability experiments showed that the synthesized conjugate was stable after 72-h incubation in phosphate-buffered saline (PBS) buffer (pH 7.4) at 37 degrees C. The synthesized conjugate may find applications in biomedical targeting, gene delivery and imaging.

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  • Authors+Show Affiliations

    ,

    Department of Physical Sciences, Charleston Southern University, Charleston, SC 29406, USA. xbi@csuniv.edu

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    Source

    MeSH

    Biocompatible Materials
    Chromatography, High Pressure Liquid
    Dendrimers
    Gonadotropin-Releasing Hormone
    Humans
    Magnetic Resonance Spectroscopy
    Models, Chemical
    Molecular Structure
    Molecular Weight
    Polyamines
    Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
    Spectrophotometry, Ultraviolet

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural

    Language

    eng

    PubMed ID

    18177559