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6,7-di-O-glucopyranosyl-esculetin protects SH-SY5Y cells from dopamine-induced cytotoxicity.
Eur J Pharmacol. 2008 Feb 12; 580(3):329-38.EJ

Abstract

Dopamine, as a neurotoxin, can elicit severe Parkinson's disease-like syndrome by elevating intracellular reactive oxygen species levels and apoptotic activity. In this study, we examined the effect of 6,7-di-O-glucopyranosyl-esculetin, which was extracted from Fraxinus sieboldiana bloom, on dopamine-induced cytotoxicity and the underlying mechanism in human neuroblastoma SH-SY5Y cells. Our results suggest that the protective effects of 6,7-di-O-glucopyranosyl-esculetin (0.1, 1 and 10 microM) on dopamine-induced cytotoxicity may be ascribed to its anti-oxidative properties by reducing reactive oxygen species levels, and its anti-apoptotic effect via protecting mitochondrion membrane potential (Delta Psi m), enhancing superoxide dismutaese (SOD) activity and reduced glutathione (GSH) levels, and regulating p53, Bax and Bcl-2 expression. In addition, 6,7-di-O-glucopyranosyl-esculetin inhibited the release of cytochrome c and apoptosis-inducing factor (AIF), and the protein expression of activated caspase 3. These data indicate that 6,7-di-O-glucopyranosyl-esculetin may provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease.

Authors+Show Affiliations

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine affiliated Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18177636

Citation

Zhao, Da-Long, et al. "6,7-di-O-glucopyranosyl-esculetin Protects SH-SY5Y Cells From Dopamine-induced Cytotoxicity." European Journal of Pharmacology, vol. 580, no. 3, 2008, pp. 329-38.
Zhao DL, Zou LB, Lin S, et al. 6,7-di-O-glucopyranosyl-esculetin protects SH-SY5Y cells from dopamine-induced cytotoxicity. Eur J Pharmacol. 2008;580(3):329-38.
Zhao, D. L., Zou, L. B., Lin, S., Shi, J. G., & Zhu, H. B. (2008). 6,7-di-O-glucopyranosyl-esculetin protects SH-SY5Y cells from dopamine-induced cytotoxicity. European Journal of Pharmacology, 580(3), 329-38. https://doi.org/10.1016/j.ejphar.2007.11.057
Zhao DL, et al. 6,7-di-O-glucopyranosyl-esculetin Protects SH-SY5Y Cells From Dopamine-induced Cytotoxicity. Eur J Pharmacol. 2008 Feb 12;580(3):329-38. PubMed PMID: 18177636.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 6,7-di-O-glucopyranosyl-esculetin protects SH-SY5Y cells from dopamine-induced cytotoxicity. AU - Zhao,Da-Long, AU - Zou,Li-Bo, AU - Lin,Sheng, AU - Shi,Jian-Gong, AU - Zhu,Hai-Bo, Y1 - 2007/12/03/ PY - 2007/02/12/received PY - 2007/10/29/revised PY - 2007/11/13/accepted PY - 2008/1/8/pubmed PY - 2008/5/31/medline PY - 2008/1/8/entrez SP - 329 EP - 38 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 580 IS - 3 N2 - Dopamine, as a neurotoxin, can elicit severe Parkinson's disease-like syndrome by elevating intracellular reactive oxygen species levels and apoptotic activity. In this study, we examined the effect of 6,7-di-O-glucopyranosyl-esculetin, which was extracted from Fraxinus sieboldiana bloom, on dopamine-induced cytotoxicity and the underlying mechanism in human neuroblastoma SH-SY5Y cells. Our results suggest that the protective effects of 6,7-di-O-glucopyranosyl-esculetin (0.1, 1 and 10 microM) on dopamine-induced cytotoxicity may be ascribed to its anti-oxidative properties by reducing reactive oxygen species levels, and its anti-apoptotic effect via protecting mitochondrion membrane potential (Delta Psi m), enhancing superoxide dismutaese (SOD) activity and reduced glutathione (GSH) levels, and regulating p53, Bax and Bcl-2 expression. In addition, 6,7-di-O-glucopyranosyl-esculetin inhibited the release of cytochrome c and apoptosis-inducing factor (AIF), and the protein expression of activated caspase 3. These data indicate that 6,7-di-O-glucopyranosyl-esculetin may provide a useful therapeutic strategy for the treatment of progressive neurodegenerative diseases such as Parkinson's disease. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/18177636/67_di_O_glucopyranosyl_esculetin_protects_SH_SY5Y_cells_from_dopamine_induced_cytotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(07)01278-2 DB - PRIME DP - Unbound Medicine ER -