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Effect of hxk2 deletion and HAP4 overexpression on fermentative capacity in Saccharomyces cerevisiae.
FEMS Yeast Res. 2008 Mar; 8(2):195-203.FY

Abstract

To describe the fermentative potential of a yeast cell, the fermentative capacity (FC) has been defined as the specific rate of ethanol and CO2 production under anaerobic conditions. The effect of growth rate on FC of glucose-limited grown Saccharomyces cerevisiae strains with altered expression of two major glycolytic regulators, Hap4p and Hxk2p, was compared with their parent strain. Whereas overproduction of Hap4p behaved similar to the wild-type strain, deletion of hxk2 resulted in a very different FC profile. Most importantly, with maltose as the carbon and energy source, the latter strain expressed an FC twofold that of the wild type. Further analysis at the level of gene expression showed large changes in ADH2 transcripts and to a lesser extent in hexose transporters and genes involved in the glyoxylate cycle. With respect to primary glucose metabolism, a shift in the type of hexose transport to one with high affinity was induced. In accordance with the phenotype of the mutant strain, the maltose transporter was constitutively expressed under glucose-limited conditions and synthesis increased in the presence of maltose.

Authors+Show Affiliations

Department of Molecular Microbial Physiology, Swammerdam Institute of Life Sciences, University of Amsterdam, Nieuwe Achtergracht, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18179578

Citation

Schuurmans, Jasper Merijn, et al. "Effect of Hxk2 Deletion and HAP4 Overexpression On Fermentative Capacity in Saccharomyces Cerevisiae." FEMS Yeast Research, vol. 8, no. 2, 2008, pp. 195-203.
Schuurmans JM, Rossell SL, van Tuijl A, et al. Effect of hxk2 deletion and HAP4 overexpression on fermentative capacity in Saccharomyces cerevisiae. FEMS Yeast Res. 2008;8(2):195-203.
Schuurmans, J. M., Rossell, S. L., van Tuijl, A., Bakker, B. M., Hellingwerf, K. J., & Teixeira de Mattos, M. J. (2008). Effect of hxk2 deletion and HAP4 overexpression on fermentative capacity in Saccharomyces cerevisiae. FEMS Yeast Research, 8(2), 195-203. https://doi.org/10.1111/j.1567-1364.2007.00319.x
Schuurmans JM, et al. Effect of Hxk2 Deletion and HAP4 Overexpression On Fermentative Capacity in Saccharomyces Cerevisiae. FEMS Yeast Res. 2008;8(2):195-203. PubMed PMID: 18179578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of hxk2 deletion and HAP4 overexpression on fermentative capacity in Saccharomyces cerevisiae. AU - Schuurmans,Jasper Merijn, AU - Rossell,Sergio L, AU - van Tuijl,Arjen, AU - Bakker,Barbara M, AU - Hellingwerf,Klaas J, AU - Teixeira de Mattos,Maarten Joost, Y1 - 2007/12/20/ PY - 2008/1/9/pubmed PY - 2008/5/6/medline PY - 2008/1/9/entrez SP - 195 EP - 203 JF - FEMS yeast research JO - FEMS Yeast Res VL - 8 IS - 2 N2 - To describe the fermentative potential of a yeast cell, the fermentative capacity (FC) has been defined as the specific rate of ethanol and CO2 production under anaerobic conditions. The effect of growth rate on FC of glucose-limited grown Saccharomyces cerevisiae strains with altered expression of two major glycolytic regulators, Hap4p and Hxk2p, was compared with their parent strain. Whereas overproduction of Hap4p behaved similar to the wild-type strain, deletion of hxk2 resulted in a very different FC profile. Most importantly, with maltose as the carbon and energy source, the latter strain expressed an FC twofold that of the wild type. Further analysis at the level of gene expression showed large changes in ADH2 transcripts and to a lesser extent in hexose transporters and genes involved in the glyoxylate cycle. With respect to primary glucose metabolism, a shift in the type of hexose transport to one with high affinity was induced. In accordance with the phenotype of the mutant strain, the maltose transporter was constitutively expressed under glucose-limited conditions and synthesis increased in the presence of maltose. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/18179578/Effect_of_hxk2_deletion_and_HAP4_overexpression_on_fermentative_capacity_in_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -