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Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence.
J Protozool. 1991 Nov-Dec; 38(6):199S-200S.JP

Abstract

The polymerase chain reaction (PCR) using published primers and probes has been compared to conventional stains and immunofluorescence for diagnosis of Pneumocystis carinii. We have screened 71 bronchoalveolar lavage (BAL) fluids from HIV-immunosuppressed patients. Of 34 samples negative by conventional stains and immunofluorescence, only one was positive by PCR. Thirty of 35 samples positive by conventional stains and immunofluorescence were also positive by PCR. One BAL sample, negative by conventional stains but positive by immunofluorescence, was negative by PCR. These data are discussed in relation to clinical and therapeutic conditions of the patients.

Authors+Show Affiliations

Laboratoire de Biochimie, CHU Saint Antoine, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1818166

Citation

Galan, F, et al. "Detection of Pneumocystis Carinii DNA By Polymerase Chain Reaction Compared to Direct Microscopy and Immunofluorescence." The Journal of Protozoology, vol. 38, no. 6, 1991, 199S-200S.
Galan F, Oliver JL, Roux P, et al. Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence. J Protozool. 1991;38(6):199S-200S.
Galan, F., Oliver, J. L., Roux, P., Poirot, J. L., & Bereziat, G. (1991). Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence. The Journal of Protozoology, 38(6), 199S-200S.
Galan F, et al. Detection of Pneumocystis Carinii DNA By Polymerase Chain Reaction Compared to Direct Microscopy and Immunofluorescence. J Protozool. 1991 Nov-Dec;38(6):199S-200S. PubMed PMID: 1818166.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence. AU - Galan,F, AU - Oliver,J L, AU - Roux,P, AU - Poirot,J L, AU - Bereziat,G, PY - 1991/11/1/pubmed PY - 1991/11/1/medline PY - 1991/11/1/entrez SP - 199S EP - 200S JF - The Journal of protozoology JO - J Protozool VL - 38 IS - 6 N2 - The polymerase chain reaction (PCR) using published primers and probes has been compared to conventional stains and immunofluorescence for diagnosis of Pneumocystis carinii. We have screened 71 bronchoalveolar lavage (BAL) fluids from HIV-immunosuppressed patients. Of 34 samples negative by conventional stains and immunofluorescence, only one was positive by PCR. Thirty of 35 samples positive by conventional stains and immunofluorescence were also positive by PCR. One BAL sample, negative by conventional stains but positive by immunofluorescence, was negative by PCR. These data are discussed in relation to clinical and therapeutic conditions of the patients. SN - 0022-3921 UR - https://www.unboundmedicine.com/medline/citation/1818166/Detection_of_Pneumocystis_carinii_DNA_by_polymerase_chain_reaction_compared_to_direct_microscopy_and_immunofluorescence_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0022-3921&date=1991&volume=38&issue=6&spage=199S DB - PRIME DP - Unbound Medicine ER -