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Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis.
J Exp Bot. 2007; 58(15-16):4225-33.JE

Abstract

The auxin indole-3-acetic acid (IAA), which is essential for plant growth and development, is suggested to be synthesized via several redundant pathways. In maize (Zea mays), the nitrilase ZmNIT2 is expressed in auxin-synthesizing tissues and efficiently hydrolyses indole-3-acetonitrile to IAA. Zmnit2 transposon insertion mutants were compromised in root growth in young seedlings and sensitivity to indole-3-acetonitrile, and accumulated lower quantities of IAA conjugates in kernels and root tips, suggesting a substantial contribution of ZmNIT2 to total IAA biosynthesis in maize. An additional enzymatic function, turnover of beta-cyanoalanine, is acquired when ZmNIT2 forms heteromers with the homologue ZmNIT1. In plants carrying an insertion mutation in either nitrilase gene this activity was strongly reduced. A dual role for ZmNIT2 in auxin biosynthesis and in cyanide detoxification as a heteromer with ZmNIT1 is therefore proposed.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kriechbaumer V
Lehrstuhl für Genetik, Technische Universität München, Am Hochanger 8, D-85350 Freising, Germany.
Park WJ
No affiliation info available
Piotrowski M
No affiliation info available
Meeley RB
No affiliation info available
Gierl A
No affiliation info available
Glawischnig E
No affiliation info available

MeSH

AlanineAminohydrolasesCyanidesFruitHomeostasisHydrolysisIndoleacetic AcidsIndolesLightMutagenesis, InsertionalPhenotypePlant RootsProtein Structure, QuaternarySeedlingsZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18182427

Citation

Kriechbaumer, Verena, et al. "Maize Nitrilases Have a Dual Role in Auxin Homeostasis and Beta-cyanoalanine Hydrolysis." Journal of Experimental Botany, vol. 58, no. 15-16, 2007, pp. 4225-33.
Kriechbaumer V, Park WJ, Piotrowski M, et al. Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis. J Exp Bot. 2007;58(15-16):4225-33.
Kriechbaumer, V., Park, W. J., Piotrowski, M., Meeley, R. B., Gierl, A., & Glawischnig, E. (2007). Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis. Journal of Experimental Botany, 58(15-16), 4225-33. https://doi.org/10.1093/jxb/erm279
Kriechbaumer V, et al. Maize Nitrilases Have a Dual Role in Auxin Homeostasis and Beta-cyanoalanine Hydrolysis. J Exp Bot. 2007;58(15-16):4225-33. PubMed PMID: 18182427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maize nitrilases have a dual role in auxin homeostasis and beta-cyanoalanine hydrolysis. AU - Kriechbaumer,Verena, AU - Park,Woong June, AU - Piotrowski,Markus, AU - Meeley,Robert B, AU - Gierl,Alfons, AU - Glawischnig,Erich, PY - 2008/1/10/pubmed PY - 2008/4/29/medline PY - 2008/1/10/entrez SP - 4225 EP - 33 JF - Journal of experimental botany JO - J Exp Bot VL - 58 IS - 15-16 N2 - The auxin indole-3-acetic acid (IAA), which is essential for plant growth and development, is suggested to be synthesized via several redundant pathways. In maize (Zea mays), the nitrilase ZmNIT2 is expressed in auxin-synthesizing tissues and efficiently hydrolyses indole-3-acetonitrile to IAA. Zmnit2 transposon insertion mutants were compromised in root growth in young seedlings and sensitivity to indole-3-acetonitrile, and accumulated lower quantities of IAA conjugates in kernels and root tips, suggesting a substantial contribution of ZmNIT2 to total IAA biosynthesis in maize. An additional enzymatic function, turnover of beta-cyanoalanine, is acquired when ZmNIT2 forms heteromers with the homologue ZmNIT1. In plants carrying an insertion mutation in either nitrilase gene this activity was strongly reduced. A dual role for ZmNIT2 in auxin biosynthesis and in cyanide detoxification as a heteromer with ZmNIT1 is therefore proposed. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/18182427/Maize_nitrilases_have_a_dual_role_in_auxin_homeostasis_and_beta_cyanoalanine_hydrolysis_ DB - PRIME DP - Unbound Medicine ER -