Tags

Type your tag names separated by a space and hit enter

Bare silica nanoparticles as concentrating and affinity probes for rapid analysis of aminothiols, lysozyme and peptide mixtures using atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2008; 22(3):283-90.RC

Abstract

The analysis of peptide mixtures from urine and plasma samples using bare (uncapped) SiO2 nanoparticles (NPs) with atmospheric-pressure matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) has been reported. The method was based on the adsorption of positively charged peptides on the surface of negatively charged SiO2 NPs through the electrostatic force of attraction. The adsorption on the surface of SiO2 NPs caused enhancement of ionization efficiency of analytes and subsequently increased the signal intensity of peptides. Maximum signal intensity was obtained at optimized concentration of SiO2 NPs and pH of the aqueous solution. The limits of detection (LODs) obtained for different peptides in deionized water with and without using SiO2 NPs were in the range 4.7-360 nM and 0.1-18.0 microM, respectively. The sensitivity of the proposed method was 21-53-fold better than conventional use of AP-MALDI-MS. In addition, linearity in the range 9.5-95 nM was obtained for the peptide angiotensin-II in deionized water with a correlation of estimation of 0.992 using an internal standard. The proposed method provided a simple way to facilitate the ionization of peptides, reduce sample complexity and increase the tolerance to salts and surfactants in the analysis of biological samples. The applicability of the present method was also demonstrated in the real-world sample analysis of aminothiols and lysozyme using MALDI-time-of-flight (TOF)-MS.

Authors+Show Affiliations

Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18186457

Citation

Agrawal, Kavita, and Hui-Fen Wu. "Bare Silica Nanoparticles as Concentrating and Affinity Probes for Rapid Analysis of Aminothiols, Lysozyme and Peptide Mixtures Using Atmospheric-pressure Matrix-assisted Laser Desorption/ionization Ion Trap and Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 3, 2008, pp. 283-90.
Agrawal K, Wu HF. Bare silica nanoparticles as concentrating and affinity probes for rapid analysis of aminothiols, lysozyme and peptide mixtures using atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2008;22(3):283-90.
Agrawal, K., & Wu, H. F. (2008). Bare silica nanoparticles as concentrating and affinity probes for rapid analysis of aminothiols, lysozyme and peptide mixtures using atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 22(3), 283-90. https://doi.org/10.1002/rcm.3363
Agrawal K, Wu HF. Bare Silica Nanoparticles as Concentrating and Affinity Probes for Rapid Analysis of Aminothiols, Lysozyme and Peptide Mixtures Using Atmospheric-pressure Matrix-assisted Laser Desorption/ionization Ion Trap and Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2008;22(3):283-90. PubMed PMID: 18186457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bare silica nanoparticles as concentrating and affinity probes for rapid analysis of aminothiols, lysozyme and peptide mixtures using atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. AU - Agrawal,Kavita, AU - Wu,Hui-Fen, PY - 2008/1/12/pubmed PY - 2008/4/12/medline PY - 2008/1/12/entrez SP - 283 EP - 90 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 3 N2 - The analysis of peptide mixtures from urine and plasma samples using bare (uncapped) SiO2 nanoparticles (NPs) with atmospheric-pressure matrix-assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) has been reported. The method was based on the adsorption of positively charged peptides on the surface of negatively charged SiO2 NPs through the electrostatic force of attraction. The adsorption on the surface of SiO2 NPs caused enhancement of ionization efficiency of analytes and subsequently increased the signal intensity of peptides. Maximum signal intensity was obtained at optimized concentration of SiO2 NPs and pH of the aqueous solution. The limits of detection (LODs) obtained for different peptides in deionized water with and without using SiO2 NPs were in the range 4.7-360 nM and 0.1-18.0 microM, respectively. The sensitivity of the proposed method was 21-53-fold better than conventional use of AP-MALDI-MS. In addition, linearity in the range 9.5-95 nM was obtained for the peptide angiotensin-II in deionized water with a correlation of estimation of 0.992 using an internal standard. The proposed method provided a simple way to facilitate the ionization of peptides, reduce sample complexity and increase the tolerance to salts and surfactants in the analysis of biological samples. The applicability of the present method was also demonstrated in the real-world sample analysis of aminothiols and lysozyme using MALDI-time-of-flight (TOF)-MS. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18186457/Bare_silica_nanoparticles_as_concentrating_and_affinity_probes_for_rapid_analysis_of_aminothiols_lysozyme_and_peptide_mixtures_using_atmospheric_pressure_matrix_assisted_laser_desorption/ionization_ion_trap_and_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -