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Impairment of arginine metabolism in rats after massive intestinal resection: effect of parenteral nutrition supplemented with citrulline compared with arginine.
Clin Sci (Lond). 2008 Sep; 115(5):159-66.CS

Abstract

Arginine homoeostasis is impaired in short bowel syndrome, but its supplementation in short bowel syndrome patients remains controversial. Recently, we demonstrated the benefits of citrulline supplementation by the enteral route in resected rats. Since the first step in managing short bowel syndrome is to initiate total parenteral nutrition, we hypothesized that parenteral citrulline supplementation would be more appropriate in this situation than arginine supplementation. In the present study, 24 rats were assigned to four groups. The sham group underwent transection whereas the three other groups underwent resection (R) of 80% of the small intestine. All rats were then fed exclusively by total parenteral nutrition as follows: supplementation with citrulline (R+CIT), with arginine (R+ARG) or no supplementation (R). All of the rats received isocaloric and isonitrogenous nutrition for 4 days. Nitrogen balance was measured daily. Rats were then killed and the blood was collected and the intestinal mucosa and extensor digitorum longus muscle were removed for amino acid and protein analysis. Citrulline and arginine increased mucosal protein content in the ileum (compared with sham and R, P<0.05). However, only citrulline prevented extensor digitorum longus atrophy (R+CIT, 130+/-3 mg compared with R, 100+/-6 mg and R+ARG, 110+/-2 mg, P<0.05). In addition, arginine worsened nitrogen balance (R+ARG, 104+/-46 mg/72 h compared with R, 249+/-69 mg/72 h, P<0.05). Only citrulline was able to prevent muscle atrophy and it was achieved independently from any noticeable effect on the gut in particular because citrulline and arginine share the same effect on mucosal ileal protein content. These results suggest that citrulline should be considered as a potential supplement for total parenteral nutrition of short bowel syndrome patients.

Authors+Show Affiliations

Laboratoire de Biologie de la Nutrition, EA 2498, Faculté de Pharmacie - Université Paris Descartes, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18186708

Citation

Osowska, Sylwia, et al. "Impairment of Arginine Metabolism in Rats After Massive Intestinal Resection: Effect of Parenteral Nutrition Supplemented With Citrulline Compared With Arginine." Clinical Science (London, England : 1979), vol. 115, no. 5, 2008, pp. 159-66.
Osowska S, Neveux N, Nakib S, et al. Impairment of arginine metabolism in rats after massive intestinal resection: effect of parenteral nutrition supplemented with citrulline compared with arginine. Clin Sci (Lond). 2008;115(5):159-66.
Osowska, S., Neveux, N., Nakib, S., Lasserre, V., Cynober, L., & Moinard, C. (2008). Impairment of arginine metabolism in rats after massive intestinal resection: effect of parenteral nutrition supplemented with citrulline compared with arginine. Clinical Science (London, England : 1979), 115(5), 159-66. https://doi.org/10.1042/CS20070451
Osowska S, et al. Impairment of Arginine Metabolism in Rats After Massive Intestinal Resection: Effect of Parenteral Nutrition Supplemented With Citrulline Compared With Arginine. Clin Sci (Lond). 2008;115(5):159-66. PubMed PMID: 18186708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impairment of arginine metabolism in rats after massive intestinal resection: effect of parenteral nutrition supplemented with citrulline compared with arginine. AU - Osowska,Sylwia, AU - Neveux,Nathalie, AU - Nakib,Samir, AU - Lasserre,Virginie, AU - Cynober,Luc, AU - Moinard,Christophe, PY - 2008/1/12/pubmed PY - 2008/8/21/medline PY - 2008/1/12/entrez SP - 159 EP - 66 JF - Clinical science (London, England : 1979) JO - Clin Sci (Lond) VL - 115 IS - 5 N2 - Arginine homoeostasis is impaired in short bowel syndrome, but its supplementation in short bowel syndrome patients remains controversial. Recently, we demonstrated the benefits of citrulline supplementation by the enteral route in resected rats. Since the first step in managing short bowel syndrome is to initiate total parenteral nutrition, we hypothesized that parenteral citrulline supplementation would be more appropriate in this situation than arginine supplementation. In the present study, 24 rats were assigned to four groups. The sham group underwent transection whereas the three other groups underwent resection (R) of 80% of the small intestine. All rats were then fed exclusively by total parenteral nutrition as follows: supplementation with citrulline (R+CIT), with arginine (R+ARG) or no supplementation (R). All of the rats received isocaloric and isonitrogenous nutrition for 4 days. Nitrogen balance was measured daily. Rats were then killed and the blood was collected and the intestinal mucosa and extensor digitorum longus muscle were removed for amino acid and protein analysis. Citrulline and arginine increased mucosal protein content in the ileum (compared with sham and R, P<0.05). However, only citrulline prevented extensor digitorum longus atrophy (R+CIT, 130+/-3 mg compared with R, 100+/-6 mg and R+ARG, 110+/-2 mg, P<0.05). In addition, arginine worsened nitrogen balance (R+ARG, 104+/-46 mg/72 h compared with R, 249+/-69 mg/72 h, P<0.05). Only citrulline was able to prevent muscle atrophy and it was achieved independently from any noticeable effect on the gut in particular because citrulline and arginine share the same effect on mucosal ileal protein content. These results suggest that citrulline should be considered as a potential supplement for total parenteral nutrition of short bowel syndrome patients. SN - 1470-8736 UR - https://www.unboundmedicine.com/medline/citation/18186708/Impairment_of_arginine_metabolism_in_rats_after_massive_intestinal_resection:_effect_of_parenteral_nutrition_supplemented_with_citrulline_compared_with_arginine_ L2 - https://portlandpress.com/clinsci/article-lookup/doi/10.1042/CS20070451 DB - PRIME DP - Unbound Medicine ER -