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Determination of caudatin-2,6-dideoxy-3-O-methy-beta-d-cymaropyranoside in rat plasma using liquid chromatography-tandem mass spectrometry.
Biomed Chromatogr. 2008 Jun; 22(6):575-80.BC

Abstract

A selective, sensitive and rapid liquid chromatography-tandem mass spectrometry method has been developed for the determination of caudatin-2,6-dideoxy-3-O-methy-beta-d-cymaropyranoside (CDMC) in rat plasma. This method involves a plasma clean-up step using liquid-liquid extraction, followed by LC separation and positive electrospray ionization mass spectrometry detection (LC/ESI-MS/MS). Chromatographic separation of the analytes was achieved using a C(18) column with a mobile phase of acetonitrile and water (70:30, v/v) at a flow rate of 1.0 mL/min. Low energy collision tandem mass spectrometric analysis (CID-MS/MS) using the multiple reaction monitoring (MRM) mode was used for analyte quantification. For the MRM analysis of CDMC, the following transition at m/z 658.4 --> 529.6 derived from the protonated molecule [M + Na](+). A calibration curve was linear in the 5-500 ng/mL range for CDMC, and the limit of detection was 5 ng/mL. The inter- and intra-day precisions (RSD) were <or=8.5 and <or=9.1%, respectively. The accuracy (RE) was +/- 10.9%. The novel developed LC/ESI-MS/MS method was successfully applied to pharmacokinetic studies of CDMC after oral administration to rats. The total chromatographic run time of this method was 5.0 min per sample, allowing for analysis of a large number of samples per batch.

Authors+Show Affiliations

Center of Drug Metabolism and Pharmacokinetic, China Pharmaceutical University, Nanjing 210009, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

18189267

Citation

Peng, Yunru, et al. "Determination of caudatin-2,6-dideoxy-3-O-methy-beta-d-cymaropyranoside in Rat Plasma Using Liquid Chromatography-tandem Mass Spectrometry." Biomedical Chromatography : BMC, vol. 22, no. 6, 2008, pp. 575-80.
Peng Y, Li Y, Wang D, et al. Determination of caudatin-2,6-dideoxy-3-O-methy-beta-d-cymaropyranoside in rat plasma using liquid chromatography-tandem mass spectrometry. Biomed Chromatogr. 2008;22(6):575-80.
Peng, Y., Li, Y., Wang, D., Liu, X., Zhang, J., Qian, S., & Duan, J. (2008). Determination of caudatin-2,6-dideoxy-3-O-methy-beta-d-cymaropyranoside in rat plasma using liquid chromatography-tandem mass spectrometry. Biomedical Chromatography : BMC, 22(6), 575-80. https://doi.org/10.1002/bmc.971
Peng Y, et al. Determination of caudatin-2,6-dideoxy-3-O-methy-beta-d-cymaropyranoside in Rat Plasma Using Liquid Chromatography-tandem Mass Spectrometry. Biomed Chromatogr. 2008;22(6):575-80. PubMed PMID: 18189267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of caudatin-2,6-dideoxy-3-O-methy-beta-d-cymaropyranoside in rat plasma using liquid chromatography-tandem mass spectrometry. AU - Peng,Yunru, AU - Li,Youbin, AU - Wang,Dawei, AU - Liu,Xiaodong, AU - Zhang,Jianfeng, AU - Qian,Shihui, AU - Duan,Jinao, PY - 2008/1/15/pubmed PY - 2008/9/6/medline PY - 2008/1/15/entrez SP - 575 EP - 80 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 22 IS - 6 N2 - A selective, sensitive and rapid liquid chromatography-tandem mass spectrometry method has been developed for the determination of caudatin-2,6-dideoxy-3-O-methy-beta-d-cymaropyranoside (CDMC) in rat plasma. This method involves a plasma clean-up step using liquid-liquid extraction, followed by LC separation and positive electrospray ionization mass spectrometry detection (LC/ESI-MS/MS). Chromatographic separation of the analytes was achieved using a C(18) column with a mobile phase of acetonitrile and water (70:30, v/v) at a flow rate of 1.0 mL/min. Low energy collision tandem mass spectrometric analysis (CID-MS/MS) using the multiple reaction monitoring (MRM) mode was used for analyte quantification. For the MRM analysis of CDMC, the following transition at m/z 658.4 --> 529.6 derived from the protonated molecule [M + Na](+). A calibration curve was linear in the 5-500 ng/mL range for CDMC, and the limit of detection was 5 ng/mL. The inter- and intra-day precisions (RSD) were <or=8.5 and <or=9.1%, respectively. The accuracy (RE) was +/- 10.9%. The novel developed LC/ESI-MS/MS method was successfully applied to pharmacokinetic studies of CDMC after oral administration to rats. The total chromatographic run time of this method was 5.0 min per sample, allowing for analysis of a large number of samples per batch. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/18189267/Determination_of_caudatin_26_dideoxy_3_O_methy_beta_d_cymaropyranoside_in_rat_plasma_using_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/bmc.971 DB - PRIME DP - Unbound Medicine ER -