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Involvement of peroxynitrite in pollen-induced nasal blockage in guinea pigs.
Eur J Pharmacol. 2008 Mar 17; 582(1-3):139-44.EJ

Abstract

Nitric oxide (NO) has been implicated in early and late phase nasal blockage in a Japanese cedar pollen-induced experimental allergic rhinitis guinea pig model. In this study, we investigated the role of peroxynitrite, which is formed by a rapid reaction of NO with superoxide anion, in the antigen-induced biphasic nasal blockage. Sensitized guinea pigs were repeatedly challenged by pollen inhalation once every week. The peroxynitrite scavenger, ebselen (30 mg/kg), or the xanthine oxidase inhibitor, allopurinol (50 mg/kg), was intraperitoneally administered 30 min before the antigen challenge. The late phase nasal blockage induced 4 h after the challenge was largely suppressed by ebselen (57% inhibition; P<0.05) and allopurinol (47% inhibition; P<0.05), but neither ebselen nor allopurinol influenced the early phase response. On the other hand, the intranasal instillation of peroxynitrite (10(-3) and 10(-2) M, 10 microl/nostril) caused a remarkable dose-dependent nasal blockage in the sensitized guinea pig. These results suggest that peroxynitrite plays a major role in the late phase nasal blockage induced by the antigen challenge in sensitized guinea pigs.

Authors+Show Affiliations

Department of Pharmacology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607-8414, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18191833

Citation

Mizutani, Nobuaki, et al. "Involvement of Peroxynitrite in Pollen-induced Nasal Blockage in Guinea Pigs." European Journal of Pharmacology, vol. 582, no. 1-3, 2008, pp. 139-44.
Mizutani N, Nabe T, Fujii M, et al. Involvement of peroxynitrite in pollen-induced nasal blockage in guinea pigs. Eur J Pharmacol. 2008;582(1-3):139-44.
Mizutani, N., Nabe, T., Fujii, M., Yoshino, S., & Kohno, S. (2008). Involvement of peroxynitrite in pollen-induced nasal blockage in guinea pigs. European Journal of Pharmacology, 582(1-3), 139-44. https://doi.org/10.1016/j.ejphar.2007.12.007
Mizutani N, et al. Involvement of Peroxynitrite in Pollen-induced Nasal Blockage in Guinea Pigs. Eur J Pharmacol. 2008 Mar 17;582(1-3):139-44. PubMed PMID: 18191833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of peroxynitrite in pollen-induced nasal blockage in guinea pigs. AU - Mizutani,Nobuaki, AU - Nabe,Takeshi, AU - Fujii,Masanori, AU - Yoshino,Shin, AU - Kohno,Shigekatsu, Y1 - 2008/01/14/ PY - 2007/06/22/received PY - 2007/11/09/revised PY - 2007/12/11/accepted PY - 2008/1/15/pubmed PY - 2008/6/6/medline PY - 2008/1/15/entrez SP - 139 EP - 44 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 582 IS - 1-3 N2 - Nitric oxide (NO) has been implicated in early and late phase nasal blockage in a Japanese cedar pollen-induced experimental allergic rhinitis guinea pig model. In this study, we investigated the role of peroxynitrite, which is formed by a rapid reaction of NO with superoxide anion, in the antigen-induced biphasic nasal blockage. Sensitized guinea pigs were repeatedly challenged by pollen inhalation once every week. The peroxynitrite scavenger, ebselen (30 mg/kg), or the xanthine oxidase inhibitor, allopurinol (50 mg/kg), was intraperitoneally administered 30 min before the antigen challenge. The late phase nasal blockage induced 4 h after the challenge was largely suppressed by ebselen (57% inhibition; P<0.05) and allopurinol (47% inhibition; P<0.05), but neither ebselen nor allopurinol influenced the early phase response. On the other hand, the intranasal instillation of peroxynitrite (10(-3) and 10(-2) M, 10 microl/nostril) caused a remarkable dose-dependent nasal blockage in the sensitized guinea pig. These results suggest that peroxynitrite plays a major role in the late phase nasal blockage induced by the antigen challenge in sensitized guinea pigs. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/18191833/Involvement_of_peroxynitrite_in_pollen_induced_nasal_blockage_in_guinea_pigs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(07)01324-6 DB - PRIME DP - Unbound Medicine ER -