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Simultaneous determination of GFA and its active metabolites in human plasma by liquid chromatography electrospray ionization mass spectrometry and its application to pharmacokinetic studies.
J Pharm Biomed Anal. 2008 Mar 13; 46(4):728-36.JP

Abstract

A sensitive and rapid liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) method has been developed and validated for simultaneous quantification of guanfu base A (GFA) and its metabolites guanfu base I (GFI) and guanfu alcohol-amine (AA) in human plasma with phenoprolamine hydrochloride (DDPH) as the internal standard. The analytes were extracted from human plasma by using liquid-liquid extraction with ethyl acetate and the LC separation was performed on a Diamonsil C(18) analytical column (150 mm x 2.1 mm i.d., 5 microm). The MS acquisition was performed in selected ion monitoring (SIM) mode of positive ions. Analysis was carried out in SIM mode at m/z 430.25 for GFA [M+H](+), m/z 388.25 for GFI [M+H](+), m/z 346.25 for AA [M+H](+) and m/z 344.20 for the IS DDPH [M+H](+). The calibration curves were linear over the range of 50-5000 ng/mL for GFA and 5-1000 ng/mL for GFI and AA, with coefficients of correlation above 0.999. The lower limit of quantification for GFA was 1 ng/mL, while for GFI and AA were both 5 ng/mL. The intra- and inter-day precisions (CV) of analysis were within 9%, and the accuracy ranged from 91% to 108%. The overall recoveries for GFA, GFI and AA were about 94.2%, 87.8% and 80.6%, respectively. The total LC-MS run-time was only 5.5 min. This quantitation method was successfully applied to the simultaneous determination of GFA and its metabolites in human plasma for the metabolic study and pharmacokinetic evaluation.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Huang Q
Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210038, Jiangsu Province, China.
Wang GJ
No affiliation info available
Sun JG
No affiliation info available
A JY
No affiliation info available
Zha WB
No affiliation info available
Zhang Y
No affiliation info available
Zhang JW
No affiliation info available
Yan B
No affiliation info available
Gu SH
No affiliation info available
Ren HC
No affiliation info available
Liu LS
No affiliation info available

MeSH

CalibrationChromatography, High Pressure LiquidDrug StabilityHeterocyclic Compounds, 4 or More RingsHumansQuality ControlSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18191932

Citation

Huang, Qing, et al. "Simultaneous Determination of GFA and Its Active Metabolites in Human Plasma By Liquid Chromatography Electrospray Ionization Mass Spectrometry and Its Application to Pharmacokinetic Studies." Journal of Pharmaceutical and Biomedical Analysis, vol. 46, no. 4, 2008, pp. 728-36.
Huang Q, Wang GJ, Sun JG, et al. Simultaneous determination of GFA and its active metabolites in human plasma by liquid chromatography electrospray ionization mass spectrometry and its application to pharmacokinetic studies. J Pharm Biomed Anal. 2008;46(4):728-36.
Huang, Q., Wang, G. J., Sun, J. G., A, J. Y., Zha, W. B., Zhang, Y., Zhang, J. W., Yan, B., Gu, S. H., Ren, H. C., & Liu, L. S. (2008). Simultaneous determination of GFA and its active metabolites in human plasma by liquid chromatography electrospray ionization mass spectrometry and its application to pharmacokinetic studies. Journal of Pharmaceutical and Biomedical Analysis, 46(4), 728-36. https://doi.org/10.1016/j.jpba.2007.11.033
Huang Q, et al. Simultaneous Determination of GFA and Its Active Metabolites in Human Plasma By Liquid Chromatography Electrospray Ionization Mass Spectrometry and Its Application to Pharmacokinetic Studies. J Pharm Biomed Anal. 2008 Mar 13;46(4):728-36. PubMed PMID: 18191932.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of GFA and its active metabolites in human plasma by liquid chromatography electrospray ionization mass spectrometry and its application to pharmacokinetic studies. AU - Huang,Qing, AU - Wang,Guang-Ji, AU - Sun,Jian-Guo, AU - A,Ji-Ye, AU - Zha,Wei-Bin, AU - Zhang,Ying, AU - Zhang,Jing-Wei, AU - Yan,Bei, AU - Gu,Sheng-Hua, AU - Ren,Hong-Can, AU - Liu,Lin-Sheng, Y1 - 2007/11/26/ PY - 2007/10/19/received PY - 2007/11/21/revised PY - 2007/11/21/accepted PY - 2008/1/15/pubmed PY - 2008/4/23/medline PY - 2008/1/15/entrez SP - 728 EP - 36 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 46 IS - 4 N2 - A sensitive and rapid liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) method has been developed and validated for simultaneous quantification of guanfu base A (GFA) and its metabolites guanfu base I (GFI) and guanfu alcohol-amine (AA) in human plasma with phenoprolamine hydrochloride (DDPH) as the internal standard. The analytes were extracted from human plasma by using liquid-liquid extraction with ethyl acetate and the LC separation was performed on a Diamonsil C(18) analytical column (150 mm x 2.1 mm i.d., 5 microm). The MS acquisition was performed in selected ion monitoring (SIM) mode of positive ions. Analysis was carried out in SIM mode at m/z 430.25 for GFA [M+H](+), m/z 388.25 for GFI [M+H](+), m/z 346.25 for AA [M+H](+) and m/z 344.20 for the IS DDPH [M+H](+). The calibration curves were linear over the range of 50-5000 ng/mL for GFA and 5-1000 ng/mL for GFI and AA, with coefficients of correlation above 0.999. The lower limit of quantification for GFA was 1 ng/mL, while for GFI and AA were both 5 ng/mL. The intra- and inter-day precisions (CV) of analysis were within 9%, and the accuracy ranged from 91% to 108%. The overall recoveries for GFA, GFI and AA were about 94.2%, 87.8% and 80.6%, respectively. The total LC-MS run-time was only 5.5 min. This quantitation method was successfully applied to the simultaneous determination of GFA and its metabolites in human plasma for the metabolic study and pharmacokinetic evaluation. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/18191932/Simultaneous_determination_of_GFA_and_its_active_metabolites_in_human_plasma_by_liquid_chromatography_electrospray_ionization_mass_spectrometry_and_its_application_to_pharmacokinetic_studies_ DB - PRIME DP - Unbound Medicine ER -