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Effect of apoE genotype and vitamin E on biomarkers of oxidative stress in cultured neuronal cells and the brain of targeted replacement mice.
J Physiol Pharmacol. 2007 Dec; 58(4):683-98.JP

Abstract

The aetiology of apoE4 genotype-Alzheimer's disease (AD) association are complex. The current study emphasizes the impact of apoE genotype and potential beneficial effects of vitamin E (VE) in relation to oxidative stress. Agonist induced neuronal cell death was examined 1) in the presence of conditioned media containing equal amounts of apoE3 or apoE4 obtained from stably transfected macrophages, and 2) after pretreatment with alpha- and gamma-tocopherol, and -tocotrienol. ApoE3 and apoE4 transgenic mice were fed a diet poor or rich in VE to study the interplay of both apoE genotype and VE status, on membrane lipid peroxidation, antioxidative enzyme activity and glutathione levels in the brain. Cytotoxicity of hydrogen peroxide and glutamate was higher in neuronal cells cultured with apoE4 than apoE3 conditioned media. VE pre-treatment of neurons counteracted the cytotoxicity of a peroxide challenge but not of nitric oxide. No significant effects of apoE genotype or VE supplementation were observed on lipid peroxidation or antioxidative status in the brain of apoE3 and apoE4 mice. VE protects against oxidative insults in vitro, however, no differences in brain oxidative status were observed in mice. Unlike in cultured cells, apoE4 may not contribute to higher neuronal oxidative stress in the brain of young targeted replacement mice.

Authors+Show Affiliations

Institute of Human Nutrition and Food Science, Christian Albrechts University of Kiel, Kiel, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18195481

Citation

Huebbe, P, et al. "Effect of apoE Genotype and Vitamin E On Biomarkers of Oxidative Stress in Cultured Neuronal Cells and the Brain of Targeted Replacement Mice." Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, vol. 58, no. 4, 2007, pp. 683-98.
Huebbe P, Jofre-Monseny L, Boesch-Saadatmandi Ch, et al. Effect of apoE genotype and vitamin E on biomarkers of oxidative stress in cultured neuronal cells and the brain of targeted replacement mice. J Physiol Pharmacol. 2007;58(4):683-98.
Huebbe, P., Jofre-Monseny, L., Boesch-Saadatmandi, C. h., Minihane, A. M., & Rimbach, G. (2007). Effect of apoE genotype and vitamin E on biomarkers of oxidative stress in cultured neuronal cells and the brain of targeted replacement mice. Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 58(4), 683-98.
Huebbe P, et al. Effect of apoE Genotype and Vitamin E On Biomarkers of Oxidative Stress in Cultured Neuronal Cells and the Brain of Targeted Replacement Mice. J Physiol Pharmacol. 2007;58(4):683-98. PubMed PMID: 18195481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of apoE genotype and vitamin E on biomarkers of oxidative stress in cultured neuronal cells and the brain of targeted replacement mice. AU - Huebbe,P, AU - Jofre-Monseny,L, AU - Boesch-Saadatmandi,Ch, AU - Minihane,A-M, AU - Rimbach,G, PY - 2007/04/17/received PY - 2007/06/26/accepted PY - 2008/1/16/pubmed PY - 2008/4/25/medline PY - 2008/1/16/entrez SP - 683 EP - 98 JF - Journal of physiology and pharmacology : an official journal of the Polish Physiological Society JO - J Physiol Pharmacol VL - 58 IS - 4 N2 - The aetiology of apoE4 genotype-Alzheimer's disease (AD) association are complex. The current study emphasizes the impact of apoE genotype and potential beneficial effects of vitamin E (VE) in relation to oxidative stress. Agonist induced neuronal cell death was examined 1) in the presence of conditioned media containing equal amounts of apoE3 or apoE4 obtained from stably transfected macrophages, and 2) after pretreatment with alpha- and gamma-tocopherol, and -tocotrienol. ApoE3 and apoE4 transgenic mice were fed a diet poor or rich in VE to study the interplay of both apoE genotype and VE status, on membrane lipid peroxidation, antioxidative enzyme activity and glutathione levels in the brain. Cytotoxicity of hydrogen peroxide and glutamate was higher in neuronal cells cultured with apoE4 than apoE3 conditioned media. VE pre-treatment of neurons counteracted the cytotoxicity of a peroxide challenge but not of nitric oxide. No significant effects of apoE genotype or VE supplementation were observed on lipid peroxidation or antioxidative status in the brain of apoE3 and apoE4 mice. VE protects against oxidative insults in vitro, however, no differences in brain oxidative status were observed in mice. Unlike in cultured cells, apoE4 may not contribute to higher neuronal oxidative stress in the brain of young targeted replacement mice. SN - 1899-1505 UR - https://www.unboundmedicine.com/medline/citation/18195481/Effect_of_apoE_genotype_and_vitamin_E_on_biomarkers_of_oxidative_stress_in_cultured_neuronal_cells_and_the_brain_of_targeted_replacement_mice_ L2 - http://www.jpp.krakow.pl/journal/archive/12_07/pdf/683_12_07_article.pdf DB - PRIME DP - Unbound Medicine ER -