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Cellular localization of facilitated glucose transporter 1 (GLUT-1) in the cochlear stria vascularis: its possible contribution to the transcellular glucose pathway.
Cell Tissue Res. 2008 Mar; 331(3):763-9.CT

Abstract

Immunoreactivity for the facilitated glucose transporter 1 (GLUT-1) has been found in the cochlear stria vascularis, but whether the strial marginal cells are immunopositive for GLUT-1 remains uncertain. To determine the cellular localization of GLUT-1 and to clarify the glucose pathway in the stria vascularis of rats and guinea pigs, immunohistochemistry was performed on sections, dissociated cells, and whole-tissue preparations. Immunoreactivity for GLUT-1 in sections was observed in the basal side of the strial tissue and in capillaries in both rats and guinea pigs. However, the distribution of the positive signals within the guinea pig strial tissue was more diffuse than that in rats. Immunostaining of dissociated guinea pig strial cells revealed GLUT-1 in the basal cells and capillary endothelial cells, but not in the marginal cells. These results indicated that GLUT-1 was not expressed in the marginal cells, and that another isoform of GLUT was probably expressed in these cells. Three-dimensional observation of whole-tissue preparations demonstrated that cytoplasmic prolongations from basal cells extended upward to the apical surface of the stria vascularis from rats and guinea pigs, and that the marginal cells were surrounded by these protrusions. We speculate that these upward extensions of basal cells have been interpreted as basal infoldings of marginal cells in previous reports from other groups. The three-dimensional relationship between marginal cells and basal cells might contribute to the transcellular glucose pathway from perilymph to intrastrial space.

Authors+Show Affiliations

Laboratory of Cell Physiology, Department of Science Education, Okayama University, Okayama, Japan. andom@cc.okayama-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18196278

Citation

Ando, Motonori, et al. "Cellular Localization of Facilitated Glucose Transporter 1 (GLUT-1) in the Cochlear Stria Vascularis: Its Possible Contribution to the Transcellular Glucose Pathway." Cell and Tissue Research, vol. 331, no. 3, 2008, pp. 763-9.
Ando M, Edamatsu M, Fukuizumi S, et al. Cellular localization of facilitated glucose transporter 1 (GLUT-1) in the cochlear stria vascularis: its possible contribution to the transcellular glucose pathway. Cell Tissue Res. 2008;331(3):763-9.
Ando, M., Edamatsu, M., Fukuizumi, S., & Takeuchi, S. (2008). Cellular localization of facilitated glucose transporter 1 (GLUT-1) in the cochlear stria vascularis: its possible contribution to the transcellular glucose pathway. Cell and Tissue Research, 331(3), 763-9. https://doi.org/10.1007/s00441-007-0495-2
Ando M, et al. Cellular Localization of Facilitated Glucose Transporter 1 (GLUT-1) in the Cochlear Stria Vascularis: Its Possible Contribution to the Transcellular Glucose Pathway. Cell Tissue Res. 2008;331(3):763-9. PubMed PMID: 18196278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular localization of facilitated glucose transporter 1 (GLUT-1) in the cochlear stria vascularis: its possible contribution to the transcellular glucose pathway. AU - Ando,Motonori, AU - Edamatsu,Midori, AU - Fukuizumi,Sho, AU - Takeuchi,Shunji, Y1 - 2008/01/15/ PY - 2007/07/01/received PY - 2007/08/02/accepted PY - 2008/1/16/pubmed PY - 2008/6/13/medline PY - 2008/1/16/entrez SP - 763 EP - 9 JF - Cell and tissue research JO - Cell Tissue Res VL - 331 IS - 3 N2 - Immunoreactivity for the facilitated glucose transporter 1 (GLUT-1) has been found in the cochlear stria vascularis, but whether the strial marginal cells are immunopositive for GLUT-1 remains uncertain. To determine the cellular localization of GLUT-1 and to clarify the glucose pathway in the stria vascularis of rats and guinea pigs, immunohistochemistry was performed on sections, dissociated cells, and whole-tissue preparations. Immunoreactivity for GLUT-1 in sections was observed in the basal side of the strial tissue and in capillaries in both rats and guinea pigs. However, the distribution of the positive signals within the guinea pig strial tissue was more diffuse than that in rats. Immunostaining of dissociated guinea pig strial cells revealed GLUT-1 in the basal cells and capillary endothelial cells, but not in the marginal cells. These results indicated that GLUT-1 was not expressed in the marginal cells, and that another isoform of GLUT was probably expressed in these cells. Three-dimensional observation of whole-tissue preparations demonstrated that cytoplasmic prolongations from basal cells extended upward to the apical surface of the stria vascularis from rats and guinea pigs, and that the marginal cells were surrounded by these protrusions. We speculate that these upward extensions of basal cells have been interpreted as basal infoldings of marginal cells in previous reports from other groups. The three-dimensional relationship between marginal cells and basal cells might contribute to the transcellular glucose pathway from perilymph to intrastrial space. SN - 0302-766X UR - https://www.unboundmedicine.com/medline/citation/18196278/Cellular_localization_of_facilitated_glucose_transporter_1__GLUT_1__in_the_cochlear_stria_vascularis:_its_possible_contribution_to_the_transcellular_glucose_pathway_ L2 - https://dx.doi.org/10.1007/s00441-007-0495-2 DB - PRIME DP - Unbound Medicine ER -