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Development of a pituitary-specific cre line targeted to the Pit-1 lineage.
Genesis. 2008 Jan; 46(1):37-42.G

Abstract

Tissue-specific expression of the Cre recombinase is a well-established genetic tool to analyze gene function in specific tissues and cell types. In this report, we describe the generation of a new transgenic line that expresses Cre under the control of the rat growth hormone releasing hormone receptor (rGhrhr) promoter. This promoter, chosen to target the anterior pituitary, drives cre-mediated recombination in cells of the Pit1 lineage, including somatotrophs, lactotrophs, and thyrotrophs. Cre activity is first detected at embryonic day 13.5, and gradually increases to reach high level expression by postnatal day 2. In addition to the pituitary, rGhrhr-cre expression was detected in vibrissae and in hair follicles of the proximal limb, but not in other tissues. The rGhrhr-cre line will be a valuable tool for the study of the development of the pituitary Pit1 lineage and for the study of tumorigenesis involving these cells.

Authors+Show Affiliations

Department of Molecular Virology, Immunology, and Molecular Genetics, The Ohio State University, Columbus, Ohio, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18196598

Citation

Yin, Zhirong, et al. "Development of a Pituitary-specific Cre Line Targeted to the Pit-1 Lineage." Genesis (New York, N.Y. : 2000), vol. 46, no. 1, 2008, pp. 37-42.
Yin Z, Williams-Simons L, Rawahneh L, et al. Development of a pituitary-specific cre line targeted to the Pit-1 lineage. Genesis. 2008;46(1):37-42.
Yin, Z., Williams-Simons, L., Rawahneh, L., Asa, S., & Kirschner, L. S. (2008). Development of a pituitary-specific cre line targeted to the Pit-1 lineage. Genesis (New York, N.Y. : 2000), 46(1), 37-42. https://doi.org/10.1002/dvg.20362
Yin Z, et al. Development of a Pituitary-specific Cre Line Targeted to the Pit-1 Lineage. Genesis. 2008;46(1):37-42. PubMed PMID: 18196598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a pituitary-specific cre line targeted to the Pit-1 lineage. AU - Yin,Zhirong, AU - Williams-Simons,Lisa, AU - Rawahneh,Lisa, AU - Asa,Sylvia, AU - Kirschner,Lawrence S, PY - 2008/1/16/pubmed PY - 2008/4/24/medline PY - 2008/1/16/entrez SP - 37 EP - 42 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 46 IS - 1 N2 - Tissue-specific expression of the Cre recombinase is a well-established genetic tool to analyze gene function in specific tissues and cell types. In this report, we describe the generation of a new transgenic line that expresses Cre under the control of the rat growth hormone releasing hormone receptor (rGhrhr) promoter. This promoter, chosen to target the anterior pituitary, drives cre-mediated recombination in cells of the Pit1 lineage, including somatotrophs, lactotrophs, and thyrotrophs. Cre activity is first detected at embryonic day 13.5, and gradually increases to reach high level expression by postnatal day 2. In addition to the pituitary, rGhrhr-cre expression was detected in vibrissae and in hair follicles of the proximal limb, but not in other tissues. The rGhrhr-cre line will be a valuable tool for the study of the development of the pituitary Pit1 lineage and for the study of tumorigenesis involving these cells. SN - 1526-968X UR - https://www.unboundmedicine.com/medline/citation/18196598/Development_of_a_pituitary_specific_cre_line_targeted_to_the_Pit_1_lineage_ L2 - https://doi.org/10.1002/dvg.20362 DB - PRIME DP - Unbound Medicine ER -