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Variability in benthic diazotrophy and cyanobacterial diversity in a tropical intertidal lagoon.
FEMS Microbiol Ecol. 2008 Feb; 63(2):205-21.FM

Abstract

Benthic nitrogen fixation has been estimated to contribute 15 Tg N year(-1) to the marine nitrogen budget. With benthic marine nitrogen fixation being largely overlooked in more recent surveys, a refocus on benthic diazotrophy was considered important. Variations in nitrogenase activity (acetylene reduction-gas chromatography) in a tropical lagoon in the western Indian Ocean (Zanzibar, Tanzania) were monitored over a 3-year period (2003-2005) and related to cyanobacterial and diazotrophic microbial diversity using a polyphasic approach. Different nitrogenase activity patterns were discerned, with the predominant pattern being high daytime activities combined with low nighttime activities. Analyses of the morphological and 16S rRNA gene diversity among cyanobacteria revealed filamentous nonheterocystous (Oscillatoriales) and unicellular (Chroococcales) representatives to be predominant. Analyses of the nifH gene diversity showed that the major phylotypes belonged to noncyanobacterial prokaryotes. However, as shown by cyanobacterial selective nifH-denaturing gradient gel electrophoresis analysis, cyanobacterial nifH gene sequences were present at all sites. Several nifH and 16S rRNA gene phylotypes were related to uncultured cyanobacteria or bacteria of geographically distant habitats, stressing the widespread occurrence of still poorly characterized microorganisms in tropical benthic marine communities.

Authors+Show Affiliations

Department of Botany, Stockholm University, Stockholm, Sweden. bauer@botan.su.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18199084

Citation

Bauer, Karolina, et al. "Variability in Benthic Diazotrophy and Cyanobacterial Diversity in a Tropical Intertidal Lagoon." FEMS Microbiology Ecology, vol. 63, no. 2, 2008, pp. 205-21.
Bauer K, Díez B, Lugomela C, et al. Variability in benthic diazotrophy and cyanobacterial diversity in a tropical intertidal lagoon. FEMS Microbiol Ecol. 2008;63(2):205-21.
Bauer, K., Díez, B., Lugomela, C., Seppälä, S., Borg, A. J., & Bergman, B. (2008). Variability in benthic diazotrophy and cyanobacterial diversity in a tropical intertidal lagoon. FEMS Microbiology Ecology, 63(2), 205-21. https://doi.org/10.1111/j.1574-6941.2007.00423.x
Bauer K, et al. Variability in Benthic Diazotrophy and Cyanobacterial Diversity in a Tropical Intertidal Lagoon. FEMS Microbiol Ecol. 2008;63(2):205-21. PubMed PMID: 18199084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variability in benthic diazotrophy and cyanobacterial diversity in a tropical intertidal lagoon. AU - Bauer,Karolina, AU - Díez,Beatriz, AU - Lugomela,Charles, AU - Seppälä,Susanna, AU - Borg,Agneta Julia, AU - Bergman,Birgitta, PY - 2008/1/18/pubmed PY - 2008/3/28/medline PY - 2008/1/18/entrez SP - 205 EP - 21 JF - FEMS microbiology ecology JO - FEMS Microbiol Ecol VL - 63 IS - 2 N2 - Benthic nitrogen fixation has been estimated to contribute 15 Tg N year(-1) to the marine nitrogen budget. With benthic marine nitrogen fixation being largely overlooked in more recent surveys, a refocus on benthic diazotrophy was considered important. Variations in nitrogenase activity (acetylene reduction-gas chromatography) in a tropical lagoon in the western Indian Ocean (Zanzibar, Tanzania) were monitored over a 3-year period (2003-2005) and related to cyanobacterial and diazotrophic microbial diversity using a polyphasic approach. Different nitrogenase activity patterns were discerned, with the predominant pattern being high daytime activities combined with low nighttime activities. Analyses of the morphological and 16S rRNA gene diversity among cyanobacteria revealed filamentous nonheterocystous (Oscillatoriales) and unicellular (Chroococcales) representatives to be predominant. Analyses of the nifH gene diversity showed that the major phylotypes belonged to noncyanobacterial prokaryotes. However, as shown by cyanobacterial selective nifH-denaturing gradient gel electrophoresis analysis, cyanobacterial nifH gene sequences were present at all sites. Several nifH and 16S rRNA gene phylotypes were related to uncultured cyanobacteria or bacteria of geographically distant habitats, stressing the widespread occurrence of still poorly characterized microorganisms in tropical benthic marine communities. SN - 0168-6496 UR - https://www.unboundmedicine.com/medline/citation/18199084/Variability_in_benthic_diazotrophy_and_cyanobacterial_diversity_in_a_tropical_intertidal_lagoon_ L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1111/j.1574-6941.2007.00423.x DB - PRIME DP - Unbound Medicine ER -