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HPV genotyping in cervical cancer in Northern Thailand: adapting the linear array HPV assay for use on paraffin-embedded tissue.
Gynecol Oncol. 2008 Mar; 108(3):555-60.GO

Abstract

OBJECTIVES

The aims of this study were to determine the prevalence of HPV infection and distribution of HPV genotypes in Northern Thai women and thereby estimate the benefit of administering the HPV vaccine in the population.

METHODS

Formaldehyde-fixed, paraffin-embedded samples of invasive squamous cell carcinoma from 99 patients were tested for HPV genotypes using the Linear Array HPV Genotyping Test.

RESULTS

HPV was detected in 96/99 (96.9%) cases. Seventy-five (78.1%) cases were single infections and 21 (21.9%) multiple. HPV16 and HPV18 were the most common subtypes, detected in 62/96 (64.4%) cases. HPV52 and HPV58 infections were found in 17/96 (17.7%) cases. Co-infection always involved HPV16. The most common co-infection was HPV16 and 52 (7 cases) but never HPV16 and 18.

CONCLUSIONS

Although the prevalence of HPV infection in cervical cancer of Northern Thai women is comparable to the other regions worldwide, the distribution of HPV subtypes differs with lower frequencies of HPV16 and 18, and higher frequencies of HPV52 and 58. Moreover, multiple infections are common. The vaccine against HPV16 and HPV18 can be estimated to prevent approximately two thirds of the cervical cancer cases in Northern Thailand. Although designed for use on unfixed tissue, this study shows that the Linear Array HPV Genotyping Test can be successfully used for HPV genotyping on paraffin-embedded archival tissue. This methodology also provides a means for retrospective studies on serial samples for a greater understanding of HPV genotypes, co-infections, and relationship to cervical cancer.

Authors+Show Affiliations

Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. ssiriaun@mail.med.cmu.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18199473

Citation

Siriaunkgul, Sumalee, et al. "HPV Genotyping in Cervical Cancer in Northern Thailand: Adapting the Linear Array HPV Assay for Use On Paraffin-embedded Tissue." Gynecologic Oncology, vol. 108, no. 3, 2008, pp. 555-60.
Siriaunkgul S, Suwiwat S, Settakorn J, et al. HPV genotyping in cervical cancer in Northern Thailand: adapting the linear array HPV assay for use on paraffin-embedded tissue. Gynecol Oncol. 2008;108(3):555-60.
Siriaunkgul, S., Suwiwat, S., Settakorn, J., Khunamornpong, S., Tungsinmunkong, K., Boonthum, A., Chaisuksunt, V., Lekawanvijit, S., Srisomboon, J., & Thorner, P. S. (2008). HPV genotyping in cervical cancer in Northern Thailand: adapting the linear array HPV assay for use on paraffin-embedded tissue. Gynecologic Oncology, 108(3), 555-60. https://doi.org/10.1016/j.ygyno.2007.11.016
Siriaunkgul S, et al. HPV Genotyping in Cervical Cancer in Northern Thailand: Adapting the Linear Array HPV Assay for Use On Paraffin-embedded Tissue. Gynecol Oncol. 2008;108(3):555-60. PubMed PMID: 18199473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPV genotyping in cervical cancer in Northern Thailand: adapting the linear array HPV assay for use on paraffin-embedded tissue. AU - Siriaunkgul,Sumalee, AU - Suwiwat,Supaporn, AU - Settakorn,Jongkolnee, AU - Khunamornpong,Surapan, AU - Tungsinmunkong,Kobkul, AU - Boonthum,Anusorn, AU - Chaisuksunt,Vipavadee, AU - Lekawanvijit,Suree, AU - Srisomboon,Jatupol, AU - Thorner,Paul S, Y1 - 2008/01/15/ PY - 2007/09/06/received PY - 2007/11/07/revised PY - 2007/11/08/accepted PY - 2008/1/18/pubmed PY - 2008/3/21/medline PY - 2008/1/18/entrez SP - 555 EP - 60 JF - Gynecologic oncology JO - Gynecol Oncol VL - 108 IS - 3 N2 - OBJECTIVES: The aims of this study were to determine the prevalence of HPV infection and distribution of HPV genotypes in Northern Thai women and thereby estimate the benefit of administering the HPV vaccine in the population. METHODS: Formaldehyde-fixed, paraffin-embedded samples of invasive squamous cell carcinoma from 99 patients were tested for HPV genotypes using the Linear Array HPV Genotyping Test. RESULTS: HPV was detected in 96/99 (96.9%) cases. Seventy-five (78.1%) cases were single infections and 21 (21.9%) multiple. HPV16 and HPV18 were the most common subtypes, detected in 62/96 (64.4%) cases. HPV52 and HPV58 infections were found in 17/96 (17.7%) cases. Co-infection always involved HPV16. The most common co-infection was HPV16 and 52 (7 cases) but never HPV16 and 18. CONCLUSIONS: Although the prevalence of HPV infection in cervical cancer of Northern Thai women is comparable to the other regions worldwide, the distribution of HPV subtypes differs with lower frequencies of HPV16 and 18, and higher frequencies of HPV52 and 58. Moreover, multiple infections are common. The vaccine against HPV16 and HPV18 can be estimated to prevent approximately two thirds of the cervical cancer cases in Northern Thailand. Although designed for use on unfixed tissue, this study shows that the Linear Array HPV Genotyping Test can be successfully used for HPV genotyping on paraffin-embedded archival tissue. This methodology also provides a means for retrospective studies on serial samples for a greater understanding of HPV genotypes, co-infections, and relationship to cervical cancer. SN - 1095-6859 UR - https://www.unboundmedicine.com/medline/citation/18199473/HPV_genotyping_in_cervical_cancer_in_Northern_Thailand:_adapting_the_linear_array_HPV_assay_for_use_on_paraffin_embedded_tissue_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0090-8258(07)00909-2 DB - PRIME DP - Unbound Medicine ER -