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Tanshinone IIA protects neonatal rat cardiomyocytes from adriamycin-induced apoptosis.
Transl Res. 2008 Feb; 151(2):79-87.TR

Abstract

Tanshinone IIA (TSN) is a monomer extracted from the Chinese herb Danshen. In this study, we examined the effect of Tanshinone IIA on adriamycin (ADR)-induced apoptosis in neonatal rat cardiomyocytes and underlying molecular mechanisms. Primary cultured cardiomyocytes were treated with 1 micromol/L of adriamycin for 24 h with or without pretreatment with Tanshinone IIA (0.5-2 micromol/L) for 2 h. 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst staining, and flow cytometry measurement were used to assess cell viability and apoptosis. Fluorescent probes 2',7'-dichlorofluorescein diacetate and dihydroethidium were used to detect the production of reactive oxygen species. Western blotting was used to evaluate the expression of Bcl-2 and Bax proteins. Adriamycin significantly induced apoptosis in cardiomyocytes. Tanshinone IIA (0.5-2 micromol/L) ameliorated apoptosis induced by adriamycin in a dose-dependent manner. Tanshinone IIA (2 micromol/L) markedly attenuated adriamycin-induced reactive oxygen species production. Western blotting revealed that Tanshinone IIA prevented the adriamycin-mediated reduction of the ratio of Bcl-2/Bax. In conclusion, Tanshinone IIA significantly inhibits adriamycin-induced cardiomyocyte apoptosis in a dose-dependent manner, and this effect is at least partly caused by its antioxidant properties.

Authors+Show Affiliations

Laboratory of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18201675

Citation

Gao, Jie, et al. "Tanshinone IIA Protects Neonatal Rat Cardiomyocytes From Adriamycin-induced Apoptosis." Translational Research : the Journal of Laboratory and Clinical Medicine, vol. 151, no. 2, 2008, pp. 79-87.
Gao J, Yang G, Pi R, et al. Tanshinone IIA protects neonatal rat cardiomyocytes from adriamycin-induced apoptosis. Transl Res. 2008;151(2):79-87.
Gao, J., Yang, G., Pi, R., Li, R., Wang, P., Zhang, H., Le, K., Chen, S., & Liu, P. (2008). Tanshinone IIA protects neonatal rat cardiomyocytes from adriamycin-induced apoptosis. Translational Research : the Journal of Laboratory and Clinical Medicine, 151(2), 79-87. https://doi.org/10.1016/j.trsl.2007.11.005
Gao J, et al. Tanshinone IIA Protects Neonatal Rat Cardiomyocytes From Adriamycin-induced Apoptosis. Transl Res. 2008;151(2):79-87. PubMed PMID: 18201675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tanshinone IIA protects neonatal rat cardiomyocytes from adriamycin-induced apoptosis. AU - Gao,Jie, AU - Yang,Guoqing, AU - Pi,Rongbiao, AU - Li,Ruifang, AU - Wang,Ping, AU - Zhang,Huijie, AU - Le,Kang, AU - Chen,Shaorui, AU - Liu,Peiqing, Y1 - 2007/12/28/ PY - 2007/07/20/received PY - 2007/11/19/revised PY - 2007/11/26/accepted PY - 2008/1/19/pubmed PY - 2008/3/5/medline PY - 2008/1/19/entrez SP - 79 EP - 87 JF - Translational research : the journal of laboratory and clinical medicine JO - Transl Res VL - 151 IS - 2 N2 - Tanshinone IIA (TSN) is a monomer extracted from the Chinese herb Danshen. In this study, we examined the effect of Tanshinone IIA on adriamycin (ADR)-induced apoptosis in neonatal rat cardiomyocytes and underlying molecular mechanisms. Primary cultured cardiomyocytes were treated with 1 micromol/L of adriamycin for 24 h with or without pretreatment with Tanshinone IIA (0.5-2 micromol/L) for 2 h. 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Hoechst staining, and flow cytometry measurement were used to assess cell viability and apoptosis. Fluorescent probes 2',7'-dichlorofluorescein diacetate and dihydroethidium were used to detect the production of reactive oxygen species. Western blotting was used to evaluate the expression of Bcl-2 and Bax proteins. Adriamycin significantly induced apoptosis in cardiomyocytes. Tanshinone IIA (0.5-2 micromol/L) ameliorated apoptosis induced by adriamycin in a dose-dependent manner. Tanshinone IIA (2 micromol/L) markedly attenuated adriamycin-induced reactive oxygen species production. Western blotting revealed that Tanshinone IIA prevented the adriamycin-mediated reduction of the ratio of Bcl-2/Bax. In conclusion, Tanshinone IIA significantly inhibits adriamycin-induced cardiomyocyte apoptosis in a dose-dependent manner, and this effect is at least partly caused by its antioxidant properties. SN - 1931-5244 UR - https://www.unboundmedicine.com/medline/citation/18201675/Tanshinone_IIA_protects_neonatal_rat_cardiomyocytes_from_adriamycin_induced_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1931-5244(07)00332-5 DB - PRIME DP - Unbound Medicine ER -