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Time dependent effects of gentamicin on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissues.
Life Sci. 2008 Feb 27; 82(9-10):450-9.LS

Abstract

Gentamicin (GM), an antibiotic against life threatening bacterial infection, induces remarkable toxicity in the kidney. Histological studies have indicated that mitochondria, microsomes, lysosomes and plasma membranes of renal proximal convoluted tubules in particular are major GM targets. Despite numerous investigations, the biochemical/cellular basis of GM nephrotoxicity is not well understood. Recently reactive oxygen species (ROS) are considered to be important mediators of GM-induced nephrotoxicity. We hypothesize that GM causes damage to intracellular organelles and affects their structural integrity and alters metabolic and other functional capabilities. To address above hypothesis a long-term, time-dependent effect of GM has been studied on blood/urine parameters, enzymes of carbohydrate metabolism, brush border membrane (BBM) and basolateral (BLM), lysosomes and oxidative stress in renal tissues. A nephrotoxic dose of GM (80 mg/kg body weight) was administered to rats daily for 15 days. The long-term treatment with GM induced a significant increase in serum creatinine, blood urea nitrogen followed by massive proteinuria, glucosuria, enzymuria along with loss of electrolytes in the urine. The activities of the enzymes of carbohydrate metabolism, plasma membranes, lysosomes significantly declined. The activities of antioxidant enzymes e.g. superoxide dismutase, catalase and glutathione peroxidase were severely depressed and lipid peroxidation was significantly increased in the renal cortex and medulla. We conclude that GM administration induced oxidative damage to renal tissues that resulted in impaired carbohydrate metabolism and decreased activities of BBM, BLM and lysosomes associated with increased lipid peroxides.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18201728

Citation

Banday, Anees A., et al. "Time Dependent Effects of Gentamicin On the Enzymes of Carbohydrate Metabolism, Brush Border Membrane and Oxidative Stress in Rat Kidney Tissues." Life Sciences, vol. 82, no. 9-10, 2008, pp. 450-9.
Banday AA, Farooq N, Priyamvada S, et al. Time dependent effects of gentamicin on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissues. Life Sci. 2008;82(9-10):450-9.
Banday, A. A., Farooq, N., Priyamvada, S., Yusufi, A. N., & Khan, F. (2008). Time dependent effects of gentamicin on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissues. Life Sciences, 82(9-10), 450-9. https://doi.org/10.1016/j.lfs.2007.11.014
Banday AA, et al. Time Dependent Effects of Gentamicin On the Enzymes of Carbohydrate Metabolism, Brush Border Membrane and Oxidative Stress in Rat Kidney Tissues. Life Sci. 2008 Feb 27;82(9-10):450-9. PubMed PMID: 18201728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time dependent effects of gentamicin on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissues. AU - Banday,Anees A, AU - Farooq,Neelam, AU - Priyamvada,Shubha, AU - Yusufi,Ahad N K, AU - Khan,Farah, Y1 - 2007/12/05/ PY - 2007/07/25/received PY - 2007/10/07/revised PY - 2007/11/28/accepted PY - 2008/1/19/pubmed PY - 2008/4/15/medline PY - 2008/1/19/entrez SP - 450 EP - 9 JF - Life sciences JO - Life Sci VL - 82 IS - 9-10 N2 - Gentamicin (GM), an antibiotic against life threatening bacterial infection, induces remarkable toxicity in the kidney. Histological studies have indicated that mitochondria, microsomes, lysosomes and plasma membranes of renal proximal convoluted tubules in particular are major GM targets. Despite numerous investigations, the biochemical/cellular basis of GM nephrotoxicity is not well understood. Recently reactive oxygen species (ROS) are considered to be important mediators of GM-induced nephrotoxicity. We hypothesize that GM causes damage to intracellular organelles and affects their structural integrity and alters metabolic and other functional capabilities. To address above hypothesis a long-term, time-dependent effect of GM has been studied on blood/urine parameters, enzymes of carbohydrate metabolism, brush border membrane (BBM) and basolateral (BLM), lysosomes and oxidative stress in renal tissues. A nephrotoxic dose of GM (80 mg/kg body weight) was administered to rats daily for 15 days. The long-term treatment with GM induced a significant increase in serum creatinine, blood urea nitrogen followed by massive proteinuria, glucosuria, enzymuria along with loss of electrolytes in the urine. The activities of the enzymes of carbohydrate metabolism, plasma membranes, lysosomes significantly declined. The activities of antioxidant enzymes e.g. superoxide dismutase, catalase and glutathione peroxidase were severely depressed and lipid peroxidation was significantly increased in the renal cortex and medulla. We conclude that GM administration induced oxidative damage to renal tissues that resulted in impaired carbohydrate metabolism and decreased activities of BBM, BLM and lysosomes associated with increased lipid peroxides. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/18201728/Time_dependent_effects_of_gentamicin_on_the_enzymes_of_carbohydrate_metabolism_brush_border_membrane_and_oxidative_stress_in_rat_kidney_tissues_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(07)00847-8 DB - PRIME DP - Unbound Medicine ER -