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Caffeine activates the PI3K/Akt pathway and prevents apoptotic cell death in a Parkinson's disease model of SH-SY5Y cells.
Neurosci Lett. 2008 Feb 20; 432(2):146-50.NL

Abstract

Parkinson's disease (PD) is one of the most common neurodegenerative diseases. Recent epidemiological studies suggest that caffeine, one of the major components of coffee, has a protective effect against developing PD. However, the detailed mechanisms of how caffeine suppresses neuronal death have not been fully elucidated. We investigated the cytoprotective mechanisms of caffeine using human dopaminergic neuroblastoma SH-SY5Y cells as a PD model. Caffeine prevented the apoptotic cell death induced by serum/retinoic acid (RA) deprivation, MPP+, rotenone, and 6-OHDA in SH-SY5Y cells in a dose dependent manner. Caffeine lowered caspase-3 activity induced by serum/RA deprivation and 6-OHDA administration, and also decreased the number of apoptotic condensed and/or fragmented nuclei. Akt was phosphorylated 60 min after caffeine administration in a dose dependent manner; PI3K inhibitors, wortmannin and LY294002 canceled this cytoprotective effect of caffeine. On the other hand, MAPKs such as Erk1/2, p38, or JNK were not activated by caffeine. These results suggest that caffeine has a cytoprotective effect due to the activation of the PI3K/Akt pathways in SH-SY5Y cells.

Authors+Show Affiliations

Department of Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University, 36-1 Nishicho, Yonago, 683-8504, Japan. kazuhiro@grape.med.tottori-u.ac.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18201823

Citation

Nakaso, Kazuhiro, et al. "Caffeine Activates the PI3K/Akt Pathway and Prevents Apoptotic Cell Death in a Parkinson's Disease Model of SH-SY5Y Cells." Neuroscience Letters, vol. 432, no. 2, 2008, pp. 146-50.
Nakaso K, Ito S, Nakashima K. Caffeine activates the PI3K/Akt pathway and prevents apoptotic cell death in a Parkinson's disease model of SH-SY5Y cells. Neurosci Lett. 2008;432(2):146-50.
Nakaso, K., Ito, S., & Nakashima, K. (2008). Caffeine activates the PI3K/Akt pathway and prevents apoptotic cell death in a Parkinson's disease model of SH-SY5Y cells. Neuroscience Letters, 432(2), 146-50. https://doi.org/10.1016/j.neulet.2007.12.034
Nakaso K, Ito S, Nakashima K. Caffeine Activates the PI3K/Akt Pathway and Prevents Apoptotic Cell Death in a Parkinson's Disease Model of SH-SY5Y Cells. Neurosci Lett. 2008 Feb 20;432(2):146-50. PubMed PMID: 18201823.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caffeine activates the PI3K/Akt pathway and prevents apoptotic cell death in a Parkinson's disease model of SH-SY5Y cells. AU - Nakaso,Kazuhiro, AU - Ito,Satoru, AU - Nakashima,Kenji, Y1 - 2007/12/23/ PY - 2007/09/25/received PY - 2007/11/22/revised PY - 2007/12/10/accepted PY - 2008/1/19/pubmed PY - 2008/6/3/medline PY - 2008/1/19/entrez SP - 146 EP - 50 JF - Neuroscience letters JO - Neurosci Lett VL - 432 IS - 2 N2 - Parkinson's disease (PD) is one of the most common neurodegenerative diseases. Recent epidemiological studies suggest that caffeine, one of the major components of coffee, has a protective effect against developing PD. However, the detailed mechanisms of how caffeine suppresses neuronal death have not been fully elucidated. We investigated the cytoprotective mechanisms of caffeine using human dopaminergic neuroblastoma SH-SY5Y cells as a PD model. Caffeine prevented the apoptotic cell death induced by serum/retinoic acid (RA) deprivation, MPP+, rotenone, and 6-OHDA in SH-SY5Y cells in a dose dependent manner. Caffeine lowered caspase-3 activity induced by serum/RA deprivation and 6-OHDA administration, and also decreased the number of apoptotic condensed and/or fragmented nuclei. Akt was phosphorylated 60 min after caffeine administration in a dose dependent manner; PI3K inhibitors, wortmannin and LY294002 canceled this cytoprotective effect of caffeine. On the other hand, MAPKs such as Erk1/2, p38, or JNK were not activated by caffeine. These results suggest that caffeine has a cytoprotective effect due to the activation of the PI3K/Akt pathways in SH-SY5Y cells. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/18201823/Caffeine_activates_the_PI3K/Akt_pathway_and_prevents_apoptotic_cell_death_in_a_Parkinson's_disease_model_of_SH_SY5Y_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(07)01275-X DB - PRIME DP - Unbound Medicine ER -