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Diversity and expression of cyanobacterial hupS genes in pure cultures and in a nitrogen-limited phototrophic biofilm.
FEMS Microbiol Ecol. 2008 Mar; 63(3):292-300.FM

Abstract

A PCR was developed for conserved regions within the cyanobacterial small subunit uptake hydrogenase (hupS) gene family. These primers were used to PCR amplify partial hupS sequences from 15 cyanobacterial strains. HupS clone libraries were constructed from PCR-amplified genomic DNA and reverse-transcribed mRNA extracted from phototrophic biofilms cultivated under nitrate-limiting conditions. Partial hupS gene sequences derived from cyanobacteria, some of which were not previously known to contain hup genes were used for phylogenetic analysis. Phylogenetic trees constructed with partial hupS genes were congruent with those based on 16S rRNA genes, indicating that hupS sequences can be used to identify cyanobacteria expressing hup. Sequences from heterocystous and nonheterocystous cyanobacteria formed two separate clusters. Analysis of clone library data showed a discrepancy between the presence and the activity of cyanobacterial hupS genes in phototrophic biofilms. The results showed that the hupS gene can be used to characterize the diversity of natural populations of diazotrophic cyanobacteria, and to characterize gene expression patterns of individual species and strains.

Authors+Show Affiliations

Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18205813

Citation

Roeselers, Guus, et al. "Diversity and Expression of Cyanobacterial hupS Genes in Pure Cultures and in a Nitrogen-limited Phototrophic Biofilm." FEMS Microbiology Ecology, vol. 63, no. 3, 2008, pp. 292-300.
Roeselers G, Huisjes EH, van Loosdrecht MC, et al. Diversity and expression of cyanobacterial hupS genes in pure cultures and in a nitrogen-limited phototrophic biofilm. FEMS Microbiol Ecol. 2008;63(3):292-300.
Roeselers, G., Huisjes, E. H., van Loosdrecht, M. C., & Muyzer, G. (2008). Diversity and expression of cyanobacterial hupS genes in pure cultures and in a nitrogen-limited phototrophic biofilm. FEMS Microbiology Ecology, 63(3), 292-300. https://doi.org/10.1111/j.1574-6941.2008.00435.x
Roeselers G, et al. Diversity and Expression of Cyanobacterial hupS Genes in Pure Cultures and in a Nitrogen-limited Phototrophic Biofilm. FEMS Microbiol Ecol. 2008;63(3):292-300. PubMed PMID: 18205813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity and expression of cyanobacterial hupS genes in pure cultures and in a nitrogen-limited phototrophic biofilm. AU - Roeselers,Guus, AU - Huisjes,Eline H, AU - van Loosdrecht,Mark C M, AU - Muyzer,Gerard, Y1 - 2008/01/17/ PY - 2008/1/22/pubmed PY - 2008/4/23/medline PY - 2008/1/22/entrez SP - 292 EP - 300 JF - FEMS microbiology ecology JO - FEMS Microbiol Ecol VL - 63 IS - 3 N2 - A PCR was developed for conserved regions within the cyanobacterial small subunit uptake hydrogenase (hupS) gene family. These primers were used to PCR amplify partial hupS sequences from 15 cyanobacterial strains. HupS clone libraries were constructed from PCR-amplified genomic DNA and reverse-transcribed mRNA extracted from phototrophic biofilms cultivated under nitrate-limiting conditions. Partial hupS gene sequences derived from cyanobacteria, some of which were not previously known to contain hup genes were used for phylogenetic analysis. Phylogenetic trees constructed with partial hupS genes were congruent with those based on 16S rRNA genes, indicating that hupS sequences can be used to identify cyanobacteria expressing hup. Sequences from heterocystous and nonheterocystous cyanobacteria formed two separate clusters. Analysis of clone library data showed a discrepancy between the presence and the activity of cyanobacterial hupS genes in phototrophic biofilms. The results showed that the hupS gene can be used to characterize the diversity of natural populations of diazotrophic cyanobacteria, and to characterize gene expression patterns of individual species and strains. SN - 0168-6496 UR - https://www.unboundmedicine.com/medline/citation/18205813/Diversity_and_expression_of_cyanobacterial_hupS_genes_in_pure_cultures_and_in_a_nitrogen_limited_phototrophic_biofilm_ L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1111/j.1574-6941.2008.00435.x DB - PRIME DP - Unbound Medicine ER -