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Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms.
Clin Exp Allergy 2008; 38(7):1220-8CE

Abstract

BACKGROUND

Green kiwifruit allergy is on the rise. However, no surveys testing purified major kiwi allergens have been carried out in a large population, including both kiwi-sensitized [skin prick test (SPT)-positive] and truly kiwi-allergic patients.

OBJECTIVE

To isolate major kiwifruit allergens, and to explore their relevance by in vitro and in vivo methods in a large kiwi-sensitized and -allergic population.

METHODS

A large group (n=92) of kiwi-sensitized patients with different clinical symptoms were selected, and double-blind, placebo-controlled, food challenges to kiwi were performed in 52 of them. The three major IgE-binding proteins from kiwifruit extracts were isolated and characterized by N-terminal amino acid sequencing and molecular size and glycosylation analysis. The allergenic potency of the three kiwi allergens, and of avocado Pers a 1 as a model allergen associated with the latex-fruit syndrome, was tested by specific IgE quantitation, immunodetection assays and SPTs.

RESULTS

The isolated kiwifruit allergens were identified as actinidin Act d 1, glycosylated thaumatin-like Act d 2 and a novel 40 kDa glycoprotein designated as Act d 3.02. Specific IgE to each of the three allergens was found in over 60% of sera from kiwi-sensitized patients, and Act d 1 and Act d 2 induced positive SPT responses in over 50% of the tested patients. A significant link between IgE levels to Act d 1 and Act d 3 and anaphylaxis was uncovered. Avocado Pers a 1 showed an in vitro sensitization prevalence of around 45%, but a low in vivo reactivity.

CONCLUSION

Act d 1, Act d 2 and Act d 3 are major allergens in the population studied. Severe symptoms after kiwi ingestion are associated with high IgE levels to Act d 1 and Act d 3.

Authors+Show Affiliations

Departamento de Biotecnología, Unidad de Bioquimica, ETS Ingenieros Agronomos, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18205857

Citation

Palacin, A, et al. "Immunoglobulin E Recognition Patterns to Purified Kiwifruit (Actinidinia Deliciosa) Allergens in Patients Sensitized to Kiwi With Different Clinical Symptoms." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 38, no. 7, 2008, pp. 1220-8.
Palacin A, Rodriguez J, Blanco C, et al. Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms. Clin Exp Allergy. 2008;38(7):1220-8.
Palacin, A., Rodriguez, J., Blanco, C., Lopez-Torrejon, G., Sánchez-Monge, R., Varela, J., ... Salcedo, G. (2008). Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 38(7), pp. 1220-8. doi:10.1111/j.1365-2222.2007.02927.x.
Palacin A, et al. Immunoglobulin E Recognition Patterns to Purified Kiwifruit (Actinidinia Deliciosa) Allergens in Patients Sensitized to Kiwi With Different Clinical Symptoms. Clin Exp Allergy. 2008;38(7):1220-8. PubMed PMID: 18205857.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoglobulin E recognition patterns to purified Kiwifruit (Actinidinia deliciosa) allergens in patients sensitized to Kiwi with different clinical symptoms. AU - Palacin,A, AU - Rodriguez,J, AU - Blanco,C, AU - Lopez-Torrejon,G, AU - Sánchez-Monge,R, AU - Varela,J, AU - Jiménez,M A, AU - Cumplido,J, AU - Carrillo,T, AU - Crespo,J F, AU - Salcedo,G, Y1 - 2008/01/19/ PY - 2008/1/22/pubmed PY - 2008/10/28/medline PY - 2008/1/22/entrez SP - 1220 EP - 8 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin. Exp. Allergy VL - 38 IS - 7 N2 - BACKGROUND: Green kiwifruit allergy is on the rise. However, no surveys testing purified major kiwi allergens have been carried out in a large population, including both kiwi-sensitized [skin prick test (SPT)-positive] and truly kiwi-allergic patients. OBJECTIVE: To isolate major kiwifruit allergens, and to explore their relevance by in vitro and in vivo methods in a large kiwi-sensitized and -allergic population. METHODS: A large group (n=92) of kiwi-sensitized patients with different clinical symptoms were selected, and double-blind, placebo-controlled, food challenges to kiwi were performed in 52 of them. The three major IgE-binding proteins from kiwifruit extracts were isolated and characterized by N-terminal amino acid sequencing and molecular size and glycosylation analysis. The allergenic potency of the three kiwi allergens, and of avocado Pers a 1 as a model allergen associated with the latex-fruit syndrome, was tested by specific IgE quantitation, immunodetection assays and SPTs. RESULTS: The isolated kiwifruit allergens were identified as actinidin Act d 1, glycosylated thaumatin-like Act d 2 and a novel 40 kDa glycoprotein designated as Act d 3.02. Specific IgE to each of the three allergens was found in over 60% of sera from kiwi-sensitized patients, and Act d 1 and Act d 2 induced positive SPT responses in over 50% of the tested patients. A significant link between IgE levels to Act d 1 and Act d 3 and anaphylaxis was uncovered. Avocado Pers a 1 showed an in vitro sensitization prevalence of around 45%, but a low in vivo reactivity. CONCLUSION: Act d 1, Act d 2 and Act d 3 are major allergens in the population studied. Severe symptoms after kiwi ingestion are associated with high IgE levels to Act d 1 and Act d 3. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/18205857/Immunoglobulin_E_recognition_patterns_to_purified_Kiwifruit__Actinidinia_deliciosa__allergens_in_patients_sensitized_to_Kiwi_with_different_clinical_symptoms_ L2 - https://doi.org/10.1111/j.1365-2222.2007.02927.x DB - PRIME DP - Unbound Medicine ER -