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Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes.
Vet Microbiol. 2008 Jun 22; 129(3-4):342-9.VM

Abstract

There are several methods for cultivation of Paenibacillus larvae, the causative agent of American foulbrood (AFB) in honey bees. Protocols for detection of sub-clinical levels of the bacterium from honey and bee samples include heat treatment of samples. The main objective of this study was to investigate if there is variability in temperature resistance among P. larvae genotypes, potentially leading to biased diagnose and disease monitoring. The variation in germination and proliferation ability among type collection (N=4) and field isolates (N=4) of P. larvae representing four different genotypes was investigated. Results demonstrate a significant variability between P. larvae genotypes in germination rate on solid media as well as in endospore resistance to heat treatment and storage. It is concluded that strains of different genotypes should be included in evaluation of standard laboratory protocols for cultivation of P. larvae to avoid bias in disease monitoring and quantification of the pathogen.

Authors+Show Affiliations

Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, Sweden. eva.forsgren@ekol.slu.seNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18207338

Citation

Forsgren, Eva, et al. "Variability in Germination and in Temperature and Storage Resistance Among Paenibacillus Larvae Genotypes." Veterinary Microbiology, vol. 129, no. 3-4, 2008, pp. 342-9.
Forsgren E, Stevanovic J, Fries I. Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes. Vet Microbiol. 2008;129(3-4):342-9.
Forsgren, E., Stevanovic, J., & Fries, I. (2008). Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes. Veterinary Microbiology, 129(3-4), 342-9. https://doi.org/10.1016/j.vetmic.2007.12.001
Forsgren E, Stevanovic J, Fries I. Variability in Germination and in Temperature and Storage Resistance Among Paenibacillus Larvae Genotypes. Vet Microbiol. 2008 Jun 22;129(3-4):342-9. PubMed PMID: 18207338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variability in germination and in temperature and storage resistance among Paenibacillus larvae genotypes. AU - Forsgren,Eva, AU - Stevanovic,Jevrosima, AU - Fries,Ingemar, Y1 - 2007/12/14/ PY - 2007/10/30/received PY - 2007/12/04/revised PY - 2007/12/05/accepted PY - 2008/1/22/pubmed PY - 2008/11/5/medline PY - 2008/1/22/entrez SP - 342 EP - 9 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 129 IS - 3-4 N2 - There are several methods for cultivation of Paenibacillus larvae, the causative agent of American foulbrood (AFB) in honey bees. Protocols for detection of sub-clinical levels of the bacterium from honey and bee samples include heat treatment of samples. The main objective of this study was to investigate if there is variability in temperature resistance among P. larvae genotypes, potentially leading to biased diagnose and disease monitoring. The variation in germination and proliferation ability among type collection (N=4) and field isolates (N=4) of P. larvae representing four different genotypes was investigated. Results demonstrate a significant variability between P. larvae genotypes in germination rate on solid media as well as in endospore resistance to heat treatment and storage. It is concluded that strains of different genotypes should be included in evaluation of standard laboratory protocols for cultivation of P. larvae to avoid bias in disease monitoring and quantification of the pathogen. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/18207338/Variability_in_germination_and_in_temperature_and_storage_resistance_among_Paenibacillus_larvae_genotypes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(07)00615-3 DB - PRIME DP - Unbound Medicine ER -