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Oxidation of guanine in G, GG, and GGG sequence contexts by aromatic pyrenyl radical cations and carbonate radical anions: relationship between kinetics and distribution of alkali-labile lesions.
J Phys Chem B 2008; 112(6):1834-44JP

Abstract

Oxidatively generated DNA damage induced by the aromatic radical cation of the pyrene derivative 7,8,9,10-tetrahydroxytetrahydrobenzo[a]pyrene (BPT), and by carbonate radicals anions, was monitored from the initial one-electron transfer, or hole injection step, to the formation of hot alkali-labile chemical end-products monitored by gel electrophoresis. The fractions of BPT molecules bound to double-stranded 20-35-mer oligonucleotides with noncontiguous guanines G and grouped as contiguous GG and GGG sequences were determined by a fluorescence quenching method. Utilizing intense nanosecond 355 nm Nd:YAG laser pulses, the DNA-bound BPT molecules were photoionized to BPT*+ radicals by a consecutive two-photon ionization mechanism. The BPT*+ radicals thus generated within the duplexes selectively oxidize guanine by intraduplex electron-transfer reactions, and the rate constants of these reactions follow the trend 5'-..GGG.. > 5'-..GG.. > 5'-..G... In the case of CO3*- radicals, the oxidation of guanine occurs by intermolecular collision pathways, and the bimolecular rate constants are independent of base sequence context. However, the distributions of the end-products generated by CO3*- radicals, as well as by BPT*+, are base sequence context-dependent and are greater than those in isolated guanines at the 5'-G in 5'-...GG... sequences, and the first two 5'- guanines in the 5'-..GGG sequences. These results help to clarify the conditions that lead to a similar or different base sequence dependence of the initial hole injection step and the final distribution of oxidized, alkali-labile guanine products. In the case of the intermolecular one-electron oxidant CO3*-, the rate constant of hole injection is similar for contiguous and isolated guanines, but the subsequent equilibration of holes by hopping favors trapping and product formation at contiguous guanines, and the sequence dependence of these two phenomena are not correlated. In contrast, in the case of the DNA-bound oxidant BPT*+, the hole injection rate constants, as well as hole equilibration, exhibit a similar dependence on base sequence context, and are thus correlated to one another.

Authors+Show Affiliations

Chemistry Department, New York University, New York, NY 10003-5180, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18211057

Citation

Lee, Young Ae, et al. "Oxidation of Guanine in G, GG, and GGG Sequence Contexts By Aromatic Pyrenyl Radical Cations and Carbonate Radical Anions: Relationship Between Kinetics and Distribution of Alkali-labile Lesions." The Journal of Physical Chemistry. B, vol. 112, no. 6, 2008, pp. 1834-44.
Lee YA, Durandin A, Dedon PC, et al. Oxidation of guanine in G, GG, and GGG sequence contexts by aromatic pyrenyl radical cations and carbonate radical anions: relationship between kinetics and distribution of alkali-labile lesions. J Phys Chem B. 2008;112(6):1834-44.
Lee, Y. A., Durandin, A., Dedon, P. C., Geacintov, N. E., & Shafirovich, V. (2008). Oxidation of guanine in G, GG, and GGG sequence contexts by aromatic pyrenyl radical cations and carbonate radical anions: relationship between kinetics and distribution of alkali-labile lesions. The Journal of Physical Chemistry. B, 112(6), pp. 1834-44. doi:10.1021/jp076777x.
Lee YA, et al. Oxidation of Guanine in G, GG, and GGG Sequence Contexts By Aromatic Pyrenyl Radical Cations and Carbonate Radical Anions: Relationship Between Kinetics and Distribution of Alkali-labile Lesions. J Phys Chem B. 2008 Feb 14;112(6):1834-44. PubMed PMID: 18211057.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidation of guanine in G, GG, and GGG sequence contexts by aromatic pyrenyl radical cations and carbonate radical anions: relationship between kinetics and distribution of alkali-labile lesions. AU - Lee,Young Ae, AU - Durandin,Alexander, AU - Dedon,Peter C, AU - Geacintov,Nicholas E, AU - Shafirovich,Vladimir, Y1 - 2008/01/23/ PY - 2008/1/24/pubmed PY - 2008/4/11/medline PY - 2008/1/24/entrez SP - 1834 EP - 44 JF - The journal of physical chemistry. B JO - J Phys Chem B VL - 112 IS - 6 N2 - Oxidatively generated DNA damage induced by the aromatic radical cation of the pyrene derivative 7,8,9,10-tetrahydroxytetrahydrobenzo[a]pyrene (BPT), and by carbonate radicals anions, was monitored from the initial one-electron transfer, or hole injection step, to the formation of hot alkali-labile chemical end-products monitored by gel electrophoresis. The fractions of BPT molecules bound to double-stranded 20-35-mer oligonucleotides with noncontiguous guanines G and grouped as contiguous GG and GGG sequences were determined by a fluorescence quenching method. Utilizing intense nanosecond 355 nm Nd:YAG laser pulses, the DNA-bound BPT molecules were photoionized to BPT*+ radicals by a consecutive two-photon ionization mechanism. The BPT*+ radicals thus generated within the duplexes selectively oxidize guanine by intraduplex electron-transfer reactions, and the rate constants of these reactions follow the trend 5'-..GGG.. > 5'-..GG.. > 5'-..G... In the case of CO3*- radicals, the oxidation of guanine occurs by intermolecular collision pathways, and the bimolecular rate constants are independent of base sequence context. However, the distributions of the end-products generated by CO3*- radicals, as well as by BPT*+, are base sequence context-dependent and are greater than those in isolated guanines at the 5'-G in 5'-...GG... sequences, and the first two 5'- guanines in the 5'-..GGG sequences. These results help to clarify the conditions that lead to a similar or different base sequence dependence of the initial hole injection step and the final distribution of oxidized, alkali-labile guanine products. In the case of the intermolecular one-electron oxidant CO3*-, the rate constant of hole injection is similar for contiguous and isolated guanines, but the subsequent equilibration of holes by hopping favors trapping and product formation at contiguous guanines, and the sequence dependence of these two phenomena are not correlated. In contrast, in the case of the DNA-bound oxidant BPT*+, the hole injection rate constants, as well as hole equilibration, exhibit a similar dependence on base sequence context, and are thus correlated to one another. SN - 1520-6106 UR - https://www.unboundmedicine.com/medline/citation/18211057/Oxidation_of_guanine_in_G_GG_and_GGG_sequence_contexts_by_aromatic_pyrenyl_radical_cations_and_carbonate_radical_anions:_relationship_between_kinetics_and_distribution_of_alkali_labile_lesions_ L2 - https://dx.doi.org/10.1021/jp076777x DB - PRIME DP - Unbound Medicine ER -