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Molecular characterization of hepatitis E virus detected in swine farms in the province of Quebec.
Can J Vet Res. 2008 Jan; 72(1):27-31.CJ

Abstract

Swine Hepatitis E virus (HEV) could be a zoonotic agent for HEV infection in humans. In Canada, approximately 60% of 6-mo-old commercial pigs are seropositive for HEV; the prevalence is higher in the provinces of Quebec and Ontario. A study was set up to evaluate the presence of swine HEV in Quebec farms and to compare the strains detected in fecal samples with human and swine HEV strains reported worldwide. Fecal samples were collected randomly from May 2003 to January 2004 from 70 swine farms in Quebec. In 24 specimens, representing 34% of the visited farms, HEV RNA was extracted and detected by nested reverse-transcription polymerase chain reaction (RT-PCR). All amplified nested RT-PCR products were purified, cloned, and sequenced. The nucleotide sequences of a 304 base pair fragment at the 5' end of the open reading frame 2 gene were determined. Phylogenetic analysis revealed that the 24 amplified fragments clustered in genotype 3 and had 85% to 99% nucleotide-sequence similarity with HEV strains identified in Japan, the United States, and Canada. Three strains identified in the study (swSTHY1, swSTHY31, and swSTHY47) showed 95% homology with 2 Japanese (swJ1-1 and HE-JA10) and 1 American (US1) HEV human strains.

Authors+Show Affiliations

Agriculture and Agri-Food Canada, Food Research and Development Centre, 3600 Casavant Blvd. West, St-Hyacinthe, Quebec J2S 8E3.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18214158

Citation

Ward, Pierre, et al. "Molecular Characterization of Hepatitis E Virus Detected in Swine Farms in the Province of Quebec." Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Veterinaire, vol. 72, no. 1, 2008, pp. 27-31.
Ward P, Müller P, Letellier A, et al. Molecular characterization of hepatitis E virus detected in swine farms in the province of Quebec. Can J Vet Res. 2008;72(1):27-31.
Ward, P., Müller, P., Letellier, A., Quessy, S., Simard, C., Trottier, Y. L., Houde, A., & Brassard, J. (2008). Molecular characterization of hepatitis E virus detected in swine farms in the province of Quebec. Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Veterinaire, 72(1), 27-31.
Ward P, et al. Molecular Characterization of Hepatitis E Virus Detected in Swine Farms in the Province of Quebec. Can J Vet Res. 2008;72(1):27-31. PubMed PMID: 18214158.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of hepatitis E virus detected in swine farms in the province of Quebec. AU - Ward,Pierre, AU - Müller,Peter, AU - Letellier,Ann, AU - Quessy,Sylvain, AU - Simard,Carole, AU - Trottier,Yvon-Louis, AU - Houde,Alain, AU - Brassard,Julie, PY - 2008/1/25/pubmed PY - 2008/2/20/medline PY - 2008/1/25/entrez SP - 27 EP - 31 JF - Canadian journal of veterinary research = Revue canadienne de recherche veterinaire JO - Can J Vet Res VL - 72 IS - 1 N2 - Swine Hepatitis E virus (HEV) could be a zoonotic agent for HEV infection in humans. In Canada, approximately 60% of 6-mo-old commercial pigs are seropositive for HEV; the prevalence is higher in the provinces of Quebec and Ontario. A study was set up to evaluate the presence of swine HEV in Quebec farms and to compare the strains detected in fecal samples with human and swine HEV strains reported worldwide. Fecal samples were collected randomly from May 2003 to January 2004 from 70 swine farms in Quebec. In 24 specimens, representing 34% of the visited farms, HEV RNA was extracted and detected by nested reverse-transcription polymerase chain reaction (RT-PCR). All amplified nested RT-PCR products were purified, cloned, and sequenced. The nucleotide sequences of a 304 base pair fragment at the 5' end of the open reading frame 2 gene were determined. Phylogenetic analysis revealed that the 24 amplified fragments clustered in genotype 3 and had 85% to 99% nucleotide-sequence similarity with HEV strains identified in Japan, the United States, and Canada. Three strains identified in the study (swSTHY1, swSTHY31, and swSTHY47) showed 95% homology with 2 Japanese (swJ1-1 and HE-JA10) and 1 American (US1) HEV human strains. SN - 0830-9000 UR - https://www.unboundmedicine.com/medline/citation/18214158/Molecular_characterization_of_hepatitis_E_virus_detected_in_swine_farms_in_the_province_of_Quebec_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18214158/ DB - PRIME DP - Unbound Medicine ER -