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Response of antioxidant enzymes, ascorbate and glutathione metabolism towards cadmium in hyperaccumulator and nonhyperaccumulator ecotypes of Sedum alfredii H.
Environ Toxicol. 2008 Aug; 23(4):517-29.ET

Abstract

Hydroponics studies were conducted to investigate the antioxidant adaptations, ascorbate and glutathione metabolism in hyperaccumulating ecotype of Sedum alfredii (HE) exposed to high Cd environment, when compared with its nonhyperaccumulating ecotype (NHE). Exposure to Cd induced a burst of oxidative stress in both ecotypes which was evident by the sharp increase in hydrogen peroxide (H(2)O(2)) contents and lipid peroxidation. Buthionine sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, did not affect H(2)O(2) concentrations as well as growth of both ecotypes in the absence of Cd. However, compared with Cd application alone, BSO combined with Cd treatment caused a substantial augmentation of H(2)O(2) accumulation accompanied by a reduction in Cd concentrations in roots and leaves of HE at the end of treatment, which may rule out the possibility that GSH biosynthesis may play an important role as a signal of the stress regulation. No efficient and superior enzymatic antioxidant defense mechanisms against Cd-imposed oxidative stress existed in both NHE and HE, but the essential nonenzymatic components like ascorbic acid (AsA) and GSH played a prominent role in tolerance against Cd. Cadmium stimulated a notable rise in AsA concentration in both ecotypes soon after the application of treatment. A preferential Cd-stress response in HE was suggested to changes in the GSH pool, where acclimation was marked by increased GSH concentrations.

Authors+Show Affiliations

Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Natural Resources Science, Zhejiang University, Hangzhou 310029, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18214940

Citation

Jin, Xiaofen, et al. "Response of Antioxidant Enzymes, Ascorbate and Glutathione Metabolism Towards Cadmium in Hyperaccumulator and Nonhyperaccumulator Ecotypes of Sedum Alfredii H." Environmental Toxicology, vol. 23, no. 4, 2008, pp. 517-29.
Jin X, Yang X, Mahmood Q, et al. Response of antioxidant enzymes, ascorbate and glutathione metabolism towards cadmium in hyperaccumulator and nonhyperaccumulator ecotypes of Sedum alfredii H. Environ Toxicol. 2008;23(4):517-29.
Jin, X., Yang, X., Mahmood, Q., Islam, E., Liu, D., & Li, H. (2008). Response of antioxidant enzymes, ascorbate and glutathione metabolism towards cadmium in hyperaccumulator and nonhyperaccumulator ecotypes of Sedum alfredii H. Environmental Toxicology, 23(4), 517-29. https://doi.org/10.1002/tox.20362
Jin X, et al. Response of Antioxidant Enzymes, Ascorbate and Glutathione Metabolism Towards Cadmium in Hyperaccumulator and Nonhyperaccumulator Ecotypes of Sedum Alfredii H. Environ Toxicol. 2008;23(4):517-29. PubMed PMID: 18214940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Response of antioxidant enzymes, ascorbate and glutathione metabolism towards cadmium in hyperaccumulator and nonhyperaccumulator ecotypes of Sedum alfredii H. AU - Jin,Xiaofen, AU - Yang,Xiaoe, AU - Mahmood,Qaisar, AU - Islam,Ejazul, AU - Liu,Dan, AU - Li,Hong, PY - 2008/1/25/pubmed PY - 2008/10/28/medline PY - 2008/1/25/entrez SP - 517 EP - 29 JF - Environmental toxicology JO - Environ Toxicol VL - 23 IS - 4 N2 - Hydroponics studies were conducted to investigate the antioxidant adaptations, ascorbate and glutathione metabolism in hyperaccumulating ecotype of Sedum alfredii (HE) exposed to high Cd environment, when compared with its nonhyperaccumulating ecotype (NHE). Exposure to Cd induced a burst of oxidative stress in both ecotypes which was evident by the sharp increase in hydrogen peroxide (H(2)O(2)) contents and lipid peroxidation. Buthionine sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, did not affect H(2)O(2) concentrations as well as growth of both ecotypes in the absence of Cd. However, compared with Cd application alone, BSO combined with Cd treatment caused a substantial augmentation of H(2)O(2) accumulation accompanied by a reduction in Cd concentrations in roots and leaves of HE at the end of treatment, which may rule out the possibility that GSH biosynthesis may play an important role as a signal of the stress regulation. No efficient and superior enzymatic antioxidant defense mechanisms against Cd-imposed oxidative stress existed in both NHE and HE, but the essential nonenzymatic components like ascorbic acid (AsA) and GSH played a prominent role in tolerance against Cd. Cadmium stimulated a notable rise in AsA concentration in both ecotypes soon after the application of treatment. A preferential Cd-stress response in HE was suggested to changes in the GSH pool, where acclimation was marked by increased GSH concentrations. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/18214940/Response_of_antioxidant_enzymes_ascorbate_and_glutathione_metabolism_towards_cadmium_in_hyperaccumulator_and_nonhyperaccumulator_ecotypes_of_Sedum_alfredii_H_ L2 - https://doi.org/10.1002/tox.20362 DB - PRIME DP - Unbound Medicine ER -