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Polypyrimidine tract binding protein induces human papillomavirus type 16 late gene expression by interfering with splicing inhibitory elements at the major late 5' splice site, SD3632.
J Virol. 2008 Apr; 82(7):3665-78.JV

Abstract

We have initiated a screen for cellular factors that can induce human papillomavirus type 16 (HPV-16) late gene expression in human cancer cells. We report that the overexpression of polypyrimidine tract binding protein (PTB), also known as heterologous nuclear ribonucleoprotein I (hnRNP I), induces HPV-16 late gene expression in cells transfected with subgenomic HPV-16 plasmids or with full-length HPV-16 genomes and in persistently HPV-16-infected cells. In contrast, other hnRNPs such as hnRNP B1/A2, hnRNP F, and hnRNP Q do not induce HPV-16 late gene expression. PTB activates SD3632, the only 5' splice site on the HPV-16 genome that is used exclusively by late mRNAs. PTB interferes with splicing inhibitory sequences located immediately upstream and downstream of SD3632, thereby activating late gene expression. One AU-rich PTB-responsive element was mapped to a 198-nucleotide sequence located downstream of SD3632. The deletion of this element induced HPV-16 late gene expression in the absence of PTB. Our results suggest that the overexpression of PTB interferes with cellular factors that interact with the inhibitory sequences. One may speculate that an increase in PTB levels or a reduction in the concentration of a PTB antagonist is required for the activation of HPV-16 late gene expression during the viral life cycle.

Authors+Show Affiliations

Department of Medical Biochemistry and Microbiology, Uppsala University, Biomedical Centre, Husargatan 3, Box 582, 751 23 Uppsala, Sweden. Stefan.Schwartz@imbim.uu.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18216120

Citation

Somberg, Monika, et al. "Polypyrimidine Tract Binding Protein Induces Human Papillomavirus Type 16 Late Gene Expression By Interfering With Splicing Inhibitory Elements at the Major Late 5' Splice Site, SD3632." Journal of Virology, vol. 82, no. 7, 2008, pp. 3665-78.
Somberg M, Zhao X, Fröhlich M, et al. Polypyrimidine tract binding protein induces human papillomavirus type 16 late gene expression by interfering with splicing inhibitory elements at the major late 5' splice site, SD3632. J Virol. 2008;82(7):3665-78.
Somberg, M., Zhao, X., Fröhlich, M., Evander, M., & Schwartz, S. (2008). Polypyrimidine tract binding protein induces human papillomavirus type 16 late gene expression by interfering with splicing inhibitory elements at the major late 5' splice site, SD3632. Journal of Virology, 82(7), 3665-78. https://doi.org/10.1128/JVI.02140-07
Somberg M, et al. Polypyrimidine Tract Binding Protein Induces Human Papillomavirus Type 16 Late Gene Expression By Interfering With Splicing Inhibitory Elements at the Major Late 5' Splice Site, SD3632. J Virol. 2008;82(7):3665-78. PubMed PMID: 18216120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polypyrimidine tract binding protein induces human papillomavirus type 16 late gene expression by interfering with splicing inhibitory elements at the major late 5' splice site, SD3632. AU - Somberg,Monika, AU - Zhao,Xiaomin, AU - Fröhlich,Monika, AU - Evander,Magnus, AU - Schwartz,Stefan, Y1 - 2008/01/23/ PY - 2008/1/25/pubmed PY - 2008/4/16/medline PY - 2008/1/25/entrez SP - 3665 EP - 78 JF - Journal of virology JO - J Virol VL - 82 IS - 7 N2 - We have initiated a screen for cellular factors that can induce human papillomavirus type 16 (HPV-16) late gene expression in human cancer cells. We report that the overexpression of polypyrimidine tract binding protein (PTB), also known as heterologous nuclear ribonucleoprotein I (hnRNP I), induces HPV-16 late gene expression in cells transfected with subgenomic HPV-16 plasmids or with full-length HPV-16 genomes and in persistently HPV-16-infected cells. In contrast, other hnRNPs such as hnRNP B1/A2, hnRNP F, and hnRNP Q do not induce HPV-16 late gene expression. PTB activates SD3632, the only 5' splice site on the HPV-16 genome that is used exclusively by late mRNAs. PTB interferes with splicing inhibitory sequences located immediately upstream and downstream of SD3632, thereby activating late gene expression. One AU-rich PTB-responsive element was mapped to a 198-nucleotide sequence located downstream of SD3632. The deletion of this element induced HPV-16 late gene expression in the absence of PTB. Our results suggest that the overexpression of PTB interferes with cellular factors that interact with the inhibitory sequences. One may speculate that an increase in PTB levels or a reduction in the concentration of a PTB antagonist is required for the activation of HPV-16 late gene expression during the viral life cycle. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/18216120/Polypyrimidine_tract_binding_protein_induces_human_papillomavirus_type_16_late_gene_expression_by_interfering_with_splicing_inhibitory_elements_at_the_major_late_5'_splice_site_SD3632_ L2 - https://journals.asm.org/doi/10.1128/JVI.02140-07?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -