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[Expression of nuclear factor-kappa B and effect of pyrrolidine dithiocarbamate in Pseudomonas aeruginosa pneumonia rat model].
Zhonghua Nei Ke Za Zhi. 2007 Oct; 46(10):815-9.ZN

Abstract

OBJECTIVE

To investigate the expression of nuclear factor-kappa B (NF-kappaB) and cytokines in Pseudomonas aeruginosa (PA)-induced pneumonia of rats, and the effect of pyrrolidine dithiocarbamate (PDTC).

METHODS

Seventy-two male SD rats were divided into three groups at random:a control group, a PA group and a PDTC group (n = 24 each). The PA induced pneumonia model was established in SD rats. The rats of the control group and the PA group were intraperitoneally given saline (1 ml) at 60 min before PA exposure, while the rats of the PDTC group received the same volume of PDTC (200 mg/kg). After 60 min, the rats of the PA group and the PDTC group were intratracheally instilled with PA 0.2 ml (6 x 10(8) CFU/ml), while the rats of the control group received the same volume of saline. At 3 h, 6 h, 16 h, 24 h after PA exposure, the rats were examined. Then they were sacrificed and the lung were excised for routine histological analysis. Immunohistochemical staining with an antibody against activated NF-kappaB and Western blot were performed to detect the expression of NF-kappaB. The change of TNFalpha mRNA was identified by reverse transcriptase polymerase chain reaction (RT-PCR).

RESULTS

Histological findings demonstrated that the lung exposed to PA showed significant changes in the lung structure, edema and pronounced inflammatory cell infiltration. Both symptoms and damages of the lung were less severe in the rats of PDTC group than those of the PA group. Compared with the PDTC group, the activation of NF-kappaB and the expression of TNFalpha in the PA group were significantly upregulated after PA challenge 3 - 24 h (P < 0.01), respectively; peak expression of NF-kappaB and TNFalpha were observed at 3 - 6 h after PA exposure.

CONCLUSIONS

The expression of NF-kappaB and TNFalpha induced by NF-kappaB play an important role in the pathogenesis of pneumonia. The inhibitor of NF-kappaB, PDTC, can relieve the lung damages produced by pneumonia.

Authors+Show Affiliations

Department of Infectious Disease, the First Affiliated Hospital of China Medical University, Shenyang 110001, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

18218230

Citation

Chai, Wen-shu, et al. "[Expression of Nuclear Factor-kappa B and Effect of Pyrrolidine Dithiocarbamate in Pseudomonas Aeruginosa Pneumonia Rat Model]." Zhonghua Nei Ke Za Zhi, vol. 46, no. 10, 2007, pp. 815-9.
Chai WS, Fan JX, Zhu XM, et al. [Expression of nuclear factor-kappa B and effect of pyrrolidine dithiocarbamate in Pseudomonas aeruginosa pneumonia rat model]. Zhonghua Nei Ke Za Zhi. 2007;46(10):815-9.
Chai, W. S., Fan, J. X., Zhu, X. M., & Chen, B. Y. (2007). [Expression of nuclear factor-kappa B and effect of pyrrolidine dithiocarbamate in Pseudomonas aeruginosa pneumonia rat model]. Zhonghua Nei Ke Za Zhi, 46(10), 815-9.
Chai WS, et al. [Expression of Nuclear Factor-kappa B and Effect of Pyrrolidine Dithiocarbamate in Pseudomonas Aeruginosa Pneumonia Rat Model]. Zhonghua Nei Ke Za Zhi. 2007;46(10):815-9. PubMed PMID: 18218230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of nuclear factor-kappa B and effect of pyrrolidine dithiocarbamate in Pseudomonas aeruginosa pneumonia rat model]. AU - Chai,Wen-shu, AU - Fan,Jin-xiu, AU - Zhu,Xiao-min, AU - Chen,Bai-yi, PY - 2008/1/26/pubmed PY - 2008/8/19/medline PY - 2008/1/26/entrez SP - 815 EP - 9 JF - Zhonghua nei ke za zhi JO - Zhonghua Nei Ke Za Zhi VL - 46 IS - 10 N2 - OBJECTIVE: To investigate the expression of nuclear factor-kappa B (NF-kappaB) and cytokines in Pseudomonas aeruginosa (PA)-induced pneumonia of rats, and the effect of pyrrolidine dithiocarbamate (PDTC). METHODS: Seventy-two male SD rats were divided into three groups at random:a control group, a PA group and a PDTC group (n = 24 each). The PA induced pneumonia model was established in SD rats. The rats of the control group and the PA group were intraperitoneally given saline (1 ml) at 60 min before PA exposure, while the rats of the PDTC group received the same volume of PDTC (200 mg/kg). After 60 min, the rats of the PA group and the PDTC group were intratracheally instilled with PA 0.2 ml (6 x 10(8) CFU/ml), while the rats of the control group received the same volume of saline. At 3 h, 6 h, 16 h, 24 h after PA exposure, the rats were examined. Then they were sacrificed and the lung were excised for routine histological analysis. Immunohistochemical staining with an antibody against activated NF-kappaB and Western blot were performed to detect the expression of NF-kappaB. The change of TNFalpha mRNA was identified by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: Histological findings demonstrated that the lung exposed to PA showed significant changes in the lung structure, edema and pronounced inflammatory cell infiltration. Both symptoms and damages of the lung were less severe in the rats of PDTC group than those of the PA group. Compared with the PDTC group, the activation of NF-kappaB and the expression of TNFalpha in the PA group were significantly upregulated after PA challenge 3 - 24 h (P < 0.01), respectively; peak expression of NF-kappaB and TNFalpha were observed at 3 - 6 h after PA exposure. CONCLUSIONS: The expression of NF-kappaB and TNFalpha induced by NF-kappaB play an important role in the pathogenesis of pneumonia. The inhibitor of NF-kappaB, PDTC, can relieve the lung damages produced by pneumonia. SN - 0578-1426 UR - https://www.unboundmedicine.com/medline/citation/18218230/[Expression_of_nuclear_factor_kappa_B_and_effect_of_pyrrolidine_dithiocarbamate_in_Pseudomonas_aeruginosa_pneumonia_rat_model]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0578-1426&amp;year=2007&amp;vol=46&amp;issue=10&amp;fpage=815 DB - PRIME DP - Unbound Medicine ER -