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A subpopulation of capsaicin-sensitive porcine dorsal root ganglion neurons is lacking hyperpolarization-activated cyclic nucleotide-gated channels.
Eur J Pain. 2008 Aug; 12(6):775-89.EJ

Abstract

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels contribute to stabilizing resting membrane potential, thus controlling neuron excitability. Subclasses of nociceptive neurons differ in their excitability, therefore, these channels could be a distinguishing marker. We investigated isolated dorsal root ganglion neurons from a non-rodent species, the pig, Sus scrofa domesticus. Single labeling revealed capsaicin-induced cobalt-uptake in 54.3% and transient receptor potential V1 (TRPV1) immunoreactivity in 55.1% of all neurons. Ruthenium red and capsazepine suppressed capsaicin-induced cobalt-uptake. HCN-1 and HCN-2 channel isoform immunoreactivity was detected in 82.6% and 88.3%, respectively, and binding of IB4 in 29.4% of all neurons. Double labeling revealed that out of the capsaicin-positive neurons, 42.3% were IB4-positive, 80.0% immunoreactive for the HCN-1, and 77.3% for the HCN-2 channel isoform, respectively. Neurons lacking HCN-1 or HCN-2 channel isoforms were mostly capsaicin-positive and IB4-negative. The soma size of neurons lacking HCN-1 and/or HCN-2 channels was small to medium. Western blot analysis showed protein products of sizes similar to those of HCN-1 and HCN-2 channel isoforms. Functionally, in patch-clamp experiments, some neurons were unresponsive to membrane hyperpolarization, thus, probably lacking HCN channels. In conclusion, in porcine dorsal root ganglion neurons there is a subset of capsaicin-positive, IB4-negative neurons lacking HCN-1 and/or HCN-2 channel isoforms.

Authors+Show Affiliations

Department of Anesthesiology and Intensive Care Medicine, Faculty of Clinical Medicine, University of Heidelberg, Mannheim, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18218331

Citation

Obreja, Otilia, et al. "A Subpopulation of Capsaicin-sensitive Porcine Dorsal Root Ganglion Neurons Is Lacking Hyperpolarization-activated Cyclic Nucleotide-gated Channels." European Journal of Pain (London, England), vol. 12, no. 6, 2008, pp. 775-89.
Obreja O, Klusch A, Ponelies N, et al. A subpopulation of capsaicin-sensitive porcine dorsal root ganglion neurons is lacking hyperpolarization-activated cyclic nucleotide-gated channels. Eur J Pain. 2008;12(6):775-89.
Obreja, O., Klusch, A., Ponelies, N., Schmelz, M., & Petersen, M. (2008). A subpopulation of capsaicin-sensitive porcine dorsal root ganglion neurons is lacking hyperpolarization-activated cyclic nucleotide-gated channels. European Journal of Pain (London, England), 12(6), 775-89. https://doi.org/10.1016/j.ejpain.2007.11.010
Obreja O, et al. A Subpopulation of Capsaicin-sensitive Porcine Dorsal Root Ganglion Neurons Is Lacking Hyperpolarization-activated Cyclic Nucleotide-gated Channels. Eur J Pain. 2008;12(6):775-89. PubMed PMID: 18218331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A subpopulation of capsaicin-sensitive porcine dorsal root ganglion neurons is lacking hyperpolarization-activated cyclic nucleotide-gated channels. AU - Obreja,Otilia, AU - Klusch,Andreas, AU - Ponelies,Norbert, AU - Schmelz,Martin, AU - Petersen,Marlen, Y1 - 2008/01/22/ PY - 2007/07/20/received PY - 2007/11/09/revised PY - 2007/11/29/accepted PY - 2008/1/26/pubmed PY - 2008/8/21/medline PY - 2008/1/26/entrez SP - 775 EP - 89 JF - European journal of pain (London, England) JO - Eur J Pain VL - 12 IS - 6 N2 - Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels contribute to stabilizing resting membrane potential, thus controlling neuron excitability. Subclasses of nociceptive neurons differ in their excitability, therefore, these channels could be a distinguishing marker. We investigated isolated dorsal root ganglion neurons from a non-rodent species, the pig, Sus scrofa domesticus. Single labeling revealed capsaicin-induced cobalt-uptake in 54.3% and transient receptor potential V1 (TRPV1) immunoreactivity in 55.1% of all neurons. Ruthenium red and capsazepine suppressed capsaicin-induced cobalt-uptake. HCN-1 and HCN-2 channel isoform immunoreactivity was detected in 82.6% and 88.3%, respectively, and binding of IB4 in 29.4% of all neurons. Double labeling revealed that out of the capsaicin-positive neurons, 42.3% were IB4-positive, 80.0% immunoreactive for the HCN-1, and 77.3% for the HCN-2 channel isoform, respectively. Neurons lacking HCN-1 or HCN-2 channel isoforms were mostly capsaicin-positive and IB4-negative. The soma size of neurons lacking HCN-1 and/or HCN-2 channels was small to medium. Western blot analysis showed protein products of sizes similar to those of HCN-1 and HCN-2 channel isoforms. Functionally, in patch-clamp experiments, some neurons were unresponsive to membrane hyperpolarization, thus, probably lacking HCN channels. In conclusion, in porcine dorsal root ganglion neurons there is a subset of capsaicin-positive, IB4-negative neurons lacking HCN-1 and/or HCN-2 channel isoforms. SN - 1532-2149 UR - https://www.unboundmedicine.com/medline/citation/18218331/A_subpopulation_of_capsaicin_sensitive_porcine_dorsal_root_ganglion_neurons_is_lacking_hyperpolarization_activated_cyclic_nucleotide_gated_channels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1090-3801(07)00705-7 DB - PRIME DP - Unbound Medicine ER -