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Temporal evolution of methane cycling and phylogenetic diversity of archaea in sediments from a deep-sea whale-fall in Monterey Canyon, California.
ISME J. 2008 Feb; 2(2):204-20.IJ

Abstract

Whale-falls represent localized areas of extreme organic enrichment in an otherwise oligotrophic deep-sea environment. Anaerobic remineralization within these habitats is typically portrayed as sulfidogenic; however, we demonstrate that these systems are also favorable for diverse methane-producing archaeal assemblages, representing up to 40% of total cell counts. Chemical analyses revealed elevated methane and depleted sulfate concentrations in sediments under the whale-fall, as compared to surrounding sediments. Carbon was enriched (up to 3.5%) in whale-fall sediments, as well as the surrounding sea floor to at least 10 m, forming a 'bulls eye' of elevated carbon. The diversity of sedimentary archaea associated with the 2893 m whale-fall in Monterey Canyon (California) varied both spatially and temporally. 16S rRNA diversity, determined by both sequencing and terminal restriction fragment length polymorphism analysis, as well as quantitative PCR of the methyl-coenzyme M reductase gene, revealed that methanogens, including members of the Methanomicrobiales and Methanosarcinales, were the dominant archaea (up to 98%) in sediments immediately beneath the whale-fall. Temporal changes in this archaeal community included the early establishment of methylotrophic methanogens followed by development of methanogens thought to be hydrogenotrophic, as well as members related to the newly described methanotrophic lineage, ANME-3. In comparison, archaeal assemblages in 'reference' sediments collected 10 m from the whale-fall primarily consisted of Crenarchaeota affiliated with marine group I and marine benthic group B. Overall, these results indicate that whale-falls can favor the establishment of metabolically and phylogenetically diverse methanogen assemblages, resulting in an active near-seafloor methane cycle in the deep sea.

Authors+Show Affiliations

Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125, USA. goffredi@caltech.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18219285

Citation

Goffredi, Shana K., et al. "Temporal Evolution of Methane Cycling and Phylogenetic Diversity of Archaea in Sediments From a Deep-sea Whale-fall in Monterey Canyon, California." The ISME Journal, vol. 2, no. 2, 2008, pp. 204-20.
Goffredi SK, Wilpiszeski R, Lee R, et al. Temporal evolution of methane cycling and phylogenetic diversity of archaea in sediments from a deep-sea whale-fall in Monterey Canyon, California. ISME J. 2008;2(2):204-20.
Goffredi, S. K., Wilpiszeski, R., Lee, R., & Orphan, V. J. (2008). Temporal evolution of methane cycling and phylogenetic diversity of archaea in sediments from a deep-sea whale-fall in Monterey Canyon, California. The ISME Journal, 2(2), 204-20. https://doi.org/10.1038/ismej.2007.103
Goffredi SK, et al. Temporal Evolution of Methane Cycling and Phylogenetic Diversity of Archaea in Sediments From a Deep-sea Whale-fall in Monterey Canyon, California. ISME J. 2008;2(2):204-20. PubMed PMID: 18219285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal evolution of methane cycling and phylogenetic diversity of archaea in sediments from a deep-sea whale-fall in Monterey Canyon, California. AU - Goffredi,Shana K, AU - Wilpiszeski,Regina, AU - Lee,Ray, AU - Orphan,Victoria J, Y1 - 2008/01/24/ PY - 2008/1/26/pubmed PY - 2008/4/2/medline PY - 2008/1/26/entrez SP - 204 EP - 20 JF - The ISME journal JO - ISME J VL - 2 IS - 2 N2 - Whale-falls represent localized areas of extreme organic enrichment in an otherwise oligotrophic deep-sea environment. Anaerobic remineralization within these habitats is typically portrayed as sulfidogenic; however, we demonstrate that these systems are also favorable for diverse methane-producing archaeal assemblages, representing up to 40% of total cell counts. Chemical analyses revealed elevated methane and depleted sulfate concentrations in sediments under the whale-fall, as compared to surrounding sediments. Carbon was enriched (up to 3.5%) in whale-fall sediments, as well as the surrounding sea floor to at least 10 m, forming a 'bulls eye' of elevated carbon. The diversity of sedimentary archaea associated with the 2893 m whale-fall in Monterey Canyon (California) varied both spatially and temporally. 16S rRNA diversity, determined by both sequencing and terminal restriction fragment length polymorphism analysis, as well as quantitative PCR of the methyl-coenzyme M reductase gene, revealed that methanogens, including members of the Methanomicrobiales and Methanosarcinales, were the dominant archaea (up to 98%) in sediments immediately beneath the whale-fall. Temporal changes in this archaeal community included the early establishment of methylotrophic methanogens followed by development of methanogens thought to be hydrogenotrophic, as well as members related to the newly described methanotrophic lineage, ANME-3. In comparison, archaeal assemblages in 'reference' sediments collected 10 m from the whale-fall primarily consisted of Crenarchaeota affiliated with marine group I and marine benthic group B. Overall, these results indicate that whale-falls can favor the establishment of metabolically and phylogenetically diverse methanogen assemblages, resulting in an active near-seafloor methane cycle in the deep sea. SN - 1751-7362 UR - https://www.unboundmedicine.com/medline/citation/18219285/Temporal_evolution_of_methane_cycling_and_phylogenetic_diversity_of_archaea_in_sediments_from_a_deep_sea_whale_fall_in_Monterey_Canyon_California_ L2 - http://dx.doi.org/10.1038/ismej.2007.103 DB - PRIME DP - Unbound Medicine ER -